##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547635_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1517480
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.266354087039037	31.0	31.0	33.0	30.0	34.0
2	31.664789651263938	31.0	31.0	34.0	30.0	34.0
3	31.809039987347443	31.0	31.0	34.0	30.0	34.0
4	35.58374937396209	37.0	35.0	37.0	33.0	37.0
5	35.47623823707726	37.0	35.0	37.0	33.0	37.0
6	35.528557872261906	37.0	35.0	37.0	33.0	37.0
7	35.83041885230777	37.0	35.0	37.0	35.0	37.0
8	35.833567493476025	37.0	35.0	37.0	35.0	37.0
9	37.66361072304083	39.0	37.0	39.0	35.0	39.0
10	37.07371431583942	39.0	37.0	39.0	33.0	39.0
11	36.78505153280439	39.0	35.0	39.0	32.0	39.0
12	36.24687837730975	38.0	35.0	39.0	32.0	39.0
13	36.055703534807705	38.0	35.0	39.0	32.0	39.0
14	37.06231581305849	39.0	35.0	41.0	31.0	41.0
15	37.26214249940691	39.0	35.0	41.0	32.0	41.0
16	37.36431056751984	39.0	35.0	41.0	32.0	41.0
17	37.24847773941008	39.0	35.0	41.0	32.0	41.0
18	37.16400216147824	39.0	35.0	41.0	32.0	41.0
19	37.120068139283546	39.0	35.0	41.0	32.0	41.0
20	36.95479281440283	39.0	35.0	41.0	31.0	41.0
21	36.82668634841975	39.0	35.0	41.0	31.0	41.0
22	36.7889863457838	38.0	35.0	41.0	31.0	41.0
23	36.7275852070538	38.0	35.0	41.0	31.0	41.0
24	36.629174684345095	38.0	35.0	40.0	31.0	41.0
25	36.56995545246066	38.0	35.0	40.0	31.0	41.0
26	36.433810659778054	38.0	35.0	40.0	31.0	41.0
27	36.344022985475924	38.0	35.0	40.0	30.0	41.0
28	36.368948519914596	38.0	35.0	40.0	30.0	41.0
29	36.37256108811978	38.0	35.0	40.0	30.0	41.0
30	36.41634881514089	38.0	35.0	40.0	30.0	41.0
31	36.35201584205393	38.0	35.0	40.0	30.0	41.0
32	36.22082861059124	38.0	35.0	41.0	30.0	41.0
33	36.10262079236629	38.0	35.0	41.0	30.0	41.0
34	35.99769749848433	38.0	35.0	41.0	30.0	41.0
35	35.834605398423705	38.0	35.0	41.0	29.0	41.0
36	35.754228721301104	38.0	35.0	40.0	29.0	41.0
37	35.71301104462662	38.0	35.0	40.0	29.0	41.0
38	35.632485436381366	38.0	35.0	40.0	28.0	41.0
39	35.57073964731001	38.0	35.0	40.0	28.0	41.0
40	35.48759192872394	38.0	34.0	40.0	27.0	41.0
41	35.403285051532805	38.0	34.0	40.0	27.0	41.0
42	35.37206948361758	38.0	34.0	40.0	27.0	41.0
43	35.32874172970978	38.0	34.0	40.0	27.0	41.0
44	35.23667527743364	38.0	34.0	40.0	27.0	41.0
45	35.146455966470725	38.0	34.0	40.0	26.0	41.0
46	35.12971109998155	38.0	34.0	40.0	26.0	41.0
47	35.05740438094736	37.0	34.0	40.0	26.0	41.0
48	34.95116640746501	37.0	34.0	40.0	26.0	41.0
49	34.886428157208	37.0	34.0	40.0	26.0	41.0
50	34.79336861111843	37.0	34.0	40.0	26.0	41.0
51	34.36881738144819	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	0.0
8	0.0
9	0.0
10	1.0
11	1.0
12	6.0
13	2.0
14	20.0
15	25.0
16	109.0
17	248.0
18	592.0
19	1176.0
20	2170.0
21	3533.0
22	5525.0
23	8069.0
24	11321.0
25	15726.0
26	20566.0
27	24362.0
28	27052.0
29	31284.0
30	37252.0
31	45965.0
32	57261.0
33	73193.0
34	118563.0
35	168616.0
36	111028.0
37	141671.0
38	218285.0
39	393732.0
40	125.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.99551888657511	23.76123573292564	28.095856288056513	13.147389092442735
2	30.418391016685558	26.942035479874527	29.239792287213014	13.399781216226902
3	28.028969080317367	26.9871761077576	31.00798692569259	13.975867886232438
4	25.42900071170625	27.88208081819859	31.802066584073597	14.886851886021562
5	23.9428526240873	32.35008039644674	29.31340116508949	14.393665814376465
6	21.653728549964416	41.73676094577853	26.616759364209088	9.992751140047975
7	87.42316208450853	3.62779081108153	7.385204417850646	1.5638426865592956
8	87.75799351556527	3.89138571842792	6.26163112528666	2.088989640720141
9	82.66132008329599	5.5690355062340196	7.961422885309856	3.808221525160134
10	41.32192846034214	33.20650025041516	13.981205683106202	11.49036560613649
11	34.04894957429423	24.050728839918815	26.48575269525793	15.414568890529035
12	33.537641352769064	22.29782270606532	27.437000817144213	16.727535124021404
13	23.874252049450405	30.876914358014602	28.36861111843319	16.8802224741018
14	17.568995966997917	33.75088963281229	29.48869177847484	19.19142262171495
15	16.40634472941983	28.243403537443655	38.50792102696576	16.842330706170756
16	19.546946252998392	25.45173577246488	37.15844689880591	17.84287107573082
17	20.14464770540633	25.694243087223555	29.388855207317395	24.77225400005272
18	22.37360624192741	26.369375543664496	32.01215172522866	19.24486648917943
19	25.229063974484013	28.381000079078472	27.053404328228382	19.336531618209136
20	26.984078867596278	27.166025252392124	28.405514405461684	17.44438147454991
21	23.254540422279042	28.388446635211007	30.167448664891793	18.189564277618157
22	22.406753301526212	24.368756095632232	30.506036323378233	22.71845427946332
23	20.027941060178716	28.773229301209902	29.78991485884493	21.408914779766455
24	20.669201571025646	26.42703692964652	34.64737591269736	18.256385586630465
25	19.2826264596568	29.88645649366054	30.64580752299866	20.185109523684
26	18.446239818646703	33.00504784247568	28.20676384532251	20.341948493555105
27	18.272267179798085	33.07615256873237	29.979505495953816	18.672074755515723
28	16.712510214302657	30.48633260405409	34.16967604185887	18.631481139784377
29	18.090320795002242	27.27166091151119	33.70897804254422	20.92904025094235
30	19.209347075414502	30.582149352874506	31.362983367161345	18.845520204549647
31	24.339694757097295	28.391807470279673	27.869296465192296	19.39920130743074
32	24.9791101035928	28.95840472362074	27.638321427630018	18.424163745156445
33	23.842884255476186	28.577312386324692	27.786923056646547	19.79288030155257
34	19.468394970609168	28.747199304109444	29.82543427260985	21.958971452671534
35	19.78826739067401	28.57935524685663	30.645543928091307	20.986833434378045
36	24.399728497245434	27.986332604054088	28.67134986952052	18.942589029179956
37	19.664048290587026	31.217215383398795	29.908137174789783	19.210599151224397
38	20.155784590241716	30.431966154413896	28.75484355642249	20.657405698921895
39	20.153544033529272	30.56231383609669	28.643211113161293	20.640931017212747
40	22.406160212984684	27.93756754619501	29.14226217149485	20.51401006932546
41	18.823707725966734	26.972348894219362	29.97561747107046	24.228325908743443
42	20.830192160687456	27.083520046392707	28.252036270659254	23.83425152226059
43	20.89325724227008	28.082281150328175	28.547328465614047	22.4771331417877
44	19.973376914358017	30.15183066663152	28.507393837151067	21.367398581859398
45	18.3882489390305	32.92807812953053	27.031196457284445	21.65247647415452
46	20.929369744576533	31.562920104383586	27.237591269736665	20.270118881303215
47	18.914318475367057	29.32091361994886	30.177333473917283	21.5874344307668
48	20.909995518886575	27.389026544007173	31.010161583678205	20.69081635342805
49	20.102538418957746	27.57301578933495	30.97965047315286	21.344795318554446
50	18.863049265888183	29.776076126209244	30.517370904394127	20.843503703508446
51	18.39497060916783	31.437251232306192	27.73255660700635	22.435221551519625
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	757.0
1	1397.5
2	2038.0
3	7560.0
4	13082.0
5	9443.5
6	5805.0
7	5907.5
8	6010.0
9	6363.5
10	6717.0
11	6691.5
12	6666.0
13	6656.0
14	6646.0
15	6685.0
16	6724.0
17	6498.5
18	6273.0
19	6212.0
20	6151.0
21	6663.5
22	7176.0
23	8463.5
24	9751.0
25	11404.5
26	15676.0
27	18294.0
28	22826.5
29	27359.0
30	31693.5
31	36028.0
32	41385.5
33	46743.0
34	53417.5
35	60092.0
36	63440.0
37	66788.0
38	73966.0
39	81144.0
40	93428.5
41	105713.0
42	115755.0
43	125797.0
44	128646.0
45	131495.0
46	140648.0
47	149801.0
48	137230.5
49	124660.0
50	117443.5
51	110227.0
52	96444.5
53	82662.0
54	73051.5
55	63441.0
56	58289.5
57	53138.0
58	47010.5
59	40883.0
60	36482.5
61	32082.0
62	26982.0
63	21882.0
64	18764.0
65	15646.0
66	12934.5
67	10223.0
68	8450.5
69	6678.0
70	5572.5
71	4467.0
72	3416.0
73	2365.0
74	1892.5
75	1086.5
76	753.0
77	612.5
78	472.0
79	367.0
80	262.0
81	157.5
82	53.0
83	42.5
84	32.0
85	18.0
86	4.0
87	5.5
88	7.0
89	8.5
90	10.0
91	6.0
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	1.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1517480.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.755009450612803
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.26929901620788	16.03725031323621
2	7.301221462396115	3.0307384090609677
3	2.554715450046146	1.590694299280029
4	1.3964764909442196	1.1593553106842351
5	0.9413383724471415	0.9768743408182448
6	0.6373586695124946	0.7937031125497096
7	0.536862141076194	0.7799805168198842
8	0.43532336652407677	0.7228112469023834
9	0.3722954083842788	0.6954295247491913
>10	6.044635425453656	31.40790831460636
>50	2.132584690424873	31.30833759685713
>100	0.3727437771315978	9.628924151915427
>500	0.003216080907069524	0.46931777081936865
>1k	0.001608040453534762	0.8893741968990567
>5k	3.2160809070695245E-4	0.5093008948018465
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7630	0.5028072857632391	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCG	3828	0.2522603263304953	No Hit
GAATCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCGTCTTC	3424	0.2256372406885099	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGC	2803	0.18471413132298284	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2019	0.1330495294830904	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.589872683659751E-5	0.0	0.0	0.035124021403906476	0.0
2	6.589872683659751E-5	0.0	0.0	0.14728365447979544	0.0
3	6.589872683659751E-5	0.0	0.0	0.2255054432348367	0.0
4	6.589872683659751E-5	0.0	0.0	0.4558873922555816	0.0
5	6.589872683659751E-5	0.0	0.0	0.9474259957297625	0.0
6	6.589872683659751E-5	0.0	0.0	1.3231805361520415	0.0
7	6.589872683659751E-5	0.0	0.0	1.537746790732003	0.0
8	6.589872683659751E-5	0.0	0.0	2.0094498774283682	0.0
9	6.589872683659751E-5	0.0	0.0	2.161280544059889	0.0
10	6.589872683659751E-5	0.0	0.0	2.686361599493898	0.0
11	6.589872683659751E-5	0.0	0.0	3.0341750797374596	0.0
12	6.589872683659751E-5	0.0	0.0	3.5017924453699556	0.0
13	6.589872683659751E-5	0.0	0.0	3.6524369349184176	0.0
14	6.589872683659751E-5	0.0	0.0	3.7243324458971454	0.0
15	6.589872683659751E-5	0.0	0.0	3.8498695205208637	0.0
16	6.589872683659751E-5	0.0	0.0	4.036824208556291	0.0
17	6.589872683659751E-5	0.0	0.0	4.227205630387221	0.0
18	6.589872683659751E-5	0.0	0.0	4.452776972348894	0.0
19	6.589872683659751E-5	0.0	0.0	4.611988296386114	0.0
20	6.589872683659751E-5	0.0	0.0	4.758547064870707	0.0
21	6.589872683659751E-5	0.0	0.0	4.9456335503598075	0.0
22	6.589872683659751E-5	0.0	0.0	5.157036666051612	0.0
23	6.589872683659751E-5	0.0	0.0	5.37535914806126	0.0
24	6.589872683659751E-5	0.0	0.0	5.539315180430714	0.0
25	6.589872683659751E-5	0.0	0.0	5.6871260247252025	0.0
26	6.589872683659751E-5	0.0	0.0	5.824524870179508	0.0
27	6.589872683659751E-5	0.0	0.0	5.98525186493397	0.0
28	6.589872683659751E-5	0.0	0.0	6.137148430292327	0.0
29	6.589872683659751E-5	0.0	0.0	6.314086511848591	0.0
30	6.589872683659751E-5	0.0	0.0	6.546643118854944	0.0
31	6.589872683659751E-5	0.0	0.0	6.748952210243298	0.0
32	6.589872683659751E-5	0.0	0.0	6.932677860663732	0.0
33	6.589872683659751E-5	0.0	0.0	7.126683712470675	0.0
34	1.3179745367319502E-4	0.0	0.0	7.330772069483618	0.0
35	1.3179745367319502E-4	0.0	0.0	7.574267865144845	0.0
36	1.3179745367319502E-4	0.0	0.0	7.787779740095421	0.0
37	1.3179745367319502E-4	0.0	0.0	8.00241189340222	0.0
38	1.3179745367319502E-4	0.0	0.0	8.23424361441337	0.0
39	1.3179745367319502E-4	0.0	0.0	8.59853177636608	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTAGT	20	7.0340006E-4	45.000004	39
GCGATCG	20	7.0340006E-4	45.000004	9
GCGTATT	20	7.0340006E-4	45.000004	16
CCGCAAT	20	7.0340006E-4	45.000004	33
CGCATCG	25	3.8913982E-5	45.0	21
TCCGTAA	25	3.8913982E-5	45.0	28
TTACGCG	25	3.8913982E-5	45.0	1
TAAGTCG	30	2.1659635E-6	44.999996	1
TATAGCG	60	0.0	44.999996	1
TACCGAT	30	2.1659635E-6	44.999996	12
TTAATCG	170	0.0	42.35294	20
GTTAGCG	75	0.0	42.0	1
CGTTTTT	4710	0.0	41.990448	1
GGGCGAT	2045	0.0	41.259167	7
ACCCGCT	105	0.0	40.714287	34
CGGTCTA	190	0.0	40.263157	31
CGCACTC	45	1.9290383E-8	40.0	32
TAATCGT	180	0.0	40.0	21
TACGGGA	620	0.0	39.55645	4
AATGCGG	200	0.0	39.375	2
>>END_MODULE