FastQCFastQC Report
Thu 26 May 2016
SRR1547634_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547634_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1511693
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121240.8020146947826048No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC45270.2994655660904694No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT16350.10815688105984482No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCATC502.1827873E-1145.0000049
TCGTTAG551.8189894E-1245.0000041
CAACGAC253.891391E-545.0000041
TCCGATC253.891391E-545.00000423
CAATTCG253.891391E-545.00000418
CGCTAGG551.8189894E-1245.0000042
GTCCGAT253.891391E-545.00000422
TCCAACG253.891391E-545.0000041
TACTAAG253.891391E-545.0000041
TAGTGCG750.045.0000041
TGTCGCG253.891391E-545.0000041
ATCGACC253.891391E-545.00000421
TACGTAG1000.045.0000041
ACGCGAG453.8562575E-1045.01
CAACGCC207.033994E-445.017
GCGCGTC406.8175723E-945.038
TGGACGC207.033994E-445.027
CATTGCG207.033994E-445.01
TAACGCG406.8175723E-945.01
CTGCGCG207.033994E-445.01