##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547634_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1511693
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.182112373345646	31.0	31.0	33.0	30.0	34.0
2	31.551496897848967	31.0	31.0	34.0	30.0	34.0
3	31.594671007936135	31.0	31.0	34.0	30.0	34.0
4	35.41544414110537	37.0	35.0	37.0	33.0	37.0
5	35.31887162274351	37.0	35.0	37.0	33.0	37.0
6	35.37708185458291	37.0	35.0	37.0	33.0	37.0
7	35.82706938511986	37.0	35.0	37.0	35.0	37.0
8	35.87607669017453	37.0	35.0	37.0	35.0	37.0
9	37.72211553536333	39.0	37.0	39.0	35.0	39.0
10	37.01345511290983	39.0	37.0	39.0	33.0	39.0
11	36.61931159302848	39.0	35.0	39.0	32.0	39.0
12	35.53307847558995	37.0	35.0	39.0	31.0	39.0
13	35.080028153864575	37.0	35.0	39.0	30.0	39.0
14	35.936811905591945	38.0	35.0	40.0	30.0	41.0
15	36.33870964541081	38.0	35.0	40.0	31.0	41.0
16	36.56723818923551	38.0	35.0	40.0	32.0	41.0
17	36.49020138348197	37.0	35.0	40.0	32.0	41.0
18	36.4030057690285	37.0	35.0	40.0	31.0	41.0
19	36.296363745813466	37.0	35.0	40.0	31.0	41.0
20	36.05599285040018	36.0	35.0	40.0	31.0	41.0
21	35.82126595810128	36.0	34.0	40.0	30.0	41.0
22	35.7203651799671	35.0	34.0	40.0	30.0	41.0
23	35.72288950203514	35.0	34.0	40.0	30.0	41.0
24	35.5929206525399	35.0	34.0	40.0	30.0	41.0
25	35.50661741504393	35.0	34.0	40.0	30.0	41.0
26	35.34562970126871	35.0	34.0	40.0	30.0	41.0
27	35.30913882646807	35.0	34.0	40.0	30.0	41.0
28	35.33316486879281	36.0	34.0	40.0	30.0	41.0
29	35.393880238910945	36.0	34.0	40.0	30.0	41.0
30	35.387063378609284	36.0	34.0	40.0	30.0	41.0
31	35.18499457231065	35.0	34.0	40.0	29.0	41.0
32	34.93899555002239	35.0	34.0	40.0	29.0	41.0
33	34.730123113621616	35.0	34.0	40.0	27.0	41.0
34	34.58742019709028	35.0	34.0	40.0	26.0	41.0
35	34.39964794439083	35.0	34.0	40.0	25.0	41.0
36	34.20371464311867	35.0	33.0	40.0	24.0	41.0
37	34.14301911829981	35.0	33.0	40.0	23.0	41.0
38	34.10199756167423	35.0	33.0	40.0	23.0	41.0
39	34.10454172904154	35.0	33.0	40.0	23.0	41.0
40	33.98493146425895	35.0	33.0	40.0	23.0	41.0
41	33.94633169565514	35.0	33.0	40.0	23.0	41.0
42	33.90263433117703	35.0	33.0	40.0	23.0	41.0
43	33.79354405954119	35.0	33.0	40.0	23.0	41.0
44	33.68386901308665	35.0	33.0	40.0	23.0	41.0
45	33.57089038581246	35.0	33.0	40.0	23.0	41.0
46	33.51306052220921	35.0	33.0	39.0	23.0	41.0
47	33.47229298541437	35.0	33.0	39.0	23.0	41.0
48	33.38906180024648	35.0	33.0	39.0	23.0	41.0
49	33.388457841638484	35.0	33.0	39.0	22.0	41.0
50	33.26552348922698	35.0	33.0	39.0	22.0	41.0
51	32.83344303373767	35.0	32.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	1.0
12	1.0
13	3.0
14	8.0
15	23.0
16	125.0
17	296.0
18	821.0
19	1691.0
20	2919.0
21	4530.0
22	7187.0
23	10921.0
24	16009.0
25	24047.0
26	30987.0
27	34616.0
28	35423.0
29	39318.0
30	45326.0
31	54184.0
32	67717.0
33	87532.0
34	156600.0
35	269084.0
36	96855.0
37	107831.0
38	152479.0
39	265052.0
40	106.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.015159824117728	24.629339422753166	29.637896054291446	14.717604698837661
2	30.68414023217677	25.87628572732691	30.51710896326172	12.922465077234596
3	27.32075891070475	24.99283915451087	34.34652406275613	13.33987787202825
4	24.263259802089447	27.633322374318066	33.38290248086086	14.720515342731627
5	21.22858278764273	31.831066228394256	33.36762160041754	13.572729383545468
6	19.555028699610304	40.32783111385711	29.609451125327695	10.507689061204887
7	86.16405579704345	3.429664621057318	8.80946065107135	1.5968189308278864
8	86.83297468467474	2.583725663874874	8.042373683016327	2.5409259684340673
9	83.51239305864353	4.267467005536177	9.938128971953962	2.2820109638663406
10	52.49240421170172	22.19590882540304	15.241255995761044	10.0704309671342
11	45.935451179571515	19.338516484497845	22.771422504437076	11.954609831493563
12	43.551501528418804	20.61304775506667	23.22627676386674	12.609173952647792
13	19.720670797575963	44.96415608195579	23.84928685917048	11.465886261297765
14	13.040610758930551	45.79507876268528	26.516627383999264	14.647683094384906
15	10.493532747720602	26.09683315329237	50.14020703939226	13.26942705959477
16	11.473692079013398	20.334353602219498	50.48214154593559	17.70981277283152
17	12.630871479857353	22.46124047673701	29.141895874360735	35.76599216904491
18	19.091442508498748	25.6628164581036	35.961534517921294	19.284206515476356
19	26.257646228433945	30.334730662905763	24.840294954068053	18.567328154592236
20	29.37818723775264	24.328352383718123	27.6243919896434	18.66906838888584
21	17.98890383166423	32.01681822962731	30.07211120247299	19.922166736235468
22	19.827636960679186	25.943825895866425	27.088899664151384	27.13963747930301
23	15.625527140762047	33.00002050680925	25.15418143763317	26.220270914795528
24	17.135688264746875	24.644884907186842	41.90076953455497	16.318657293511315
25	13.320363327739166	28.625918093157804	36.34620256890784	21.707516010195192
26	12.72421053745701	39.400658731634	28.478533670527018	19.39659706038197
27	13.859758562088995	39.18011130566855	29.78461896694633	17.17551116529613
28	11.210940316585443	31.266202859972232	41.358397505313576	16.164459318128745
29	10.852137305656639	25.540767867549825	41.69093857019911	21.916156256594427
30	14.648939963339117	35.46904034086286	33.144626587541254	16.737393108256768
31	27.160607345539074	31.378394951885074	25.093322519850258	16.367675182725595
32	26.29832909195187	30.57373421719886	25.804445743944044	17.323490946905228
33	24.255520135371402	33.716700414700604	23.10654345822862	18.921235991699373
34	14.802807183733735	31.611841822380605	27.319502041750543	26.265848952135123
35	15.398166162044808	30.335789078867204	30.566126852476	23.699917906611994
36	26.508556962293273	28.079378551068242	27.348277725702243	18.06378676093625
37	15.004435424388419	34.816460749636335	32.003257275121335	18.17584655085391
38	14.57240325912735	35.84801940605665	26.661762672711987	22.91781466210401
39	15.567578866873102	35.14503275466645	28.877358034997847	20.410030343462594
40	22.064599095186654	29.92413142086389	25.572784950383443	22.438484533566008
41	13.672948144894498	25.08849349702618	30.65000631742027	30.58855204065905
42	19.894317166250026	24.951759385007406	25.392060424967237	29.76186302377533
43	20.427295753833615	27.750211187059808	25.490360807386153	26.332132251720424
44	15.97116610317042	34.30061527042859	27.40271999671891	22.325498629682087
45	13.980219528700601	42.098759470342195	21.69600573661451	22.225015264342694
46	20.68468928545677	37.925491485374344	22.952808539829185	18.4370106893397
47	17.47656435532876	30.76107384237408	27.295687682618098	24.46667411967906
48	20.07947380850477	26.127130310188644	32.928246674423974	20.865149206882613
49	18.252317104068087	25.9700878419097	31.99161469954548	23.785980354476735
50	16.046512089425566	34.2637030137733	29.565460711930264	20.124324184870872
51	14.738309961083369	36.38543011047879	24.30983010439289	24.56642982404496
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1089.0
1	1233.5
2	1378.0
3	8757.5
4	16137.0
5	11728.0
6	7319.0
7	7945.0
8	8571.0
9	10048.0
10	11525.0
11	12260.0
12	12995.0
13	12754.5
14	12514.0
15	12009.0
16	11504.0
17	10645.5
18	9787.0
19	9297.5
20	8808.0
21	8768.5
22	8729.0
23	8322.0
24	7915.0
25	8440.0
26	10849.5
27	12734.0
28	14674.0
29	16614.0
30	21362.5
31	26111.0
32	30511.5
33	34912.0
34	39751.0
35	44590.0
36	48266.0
37	51942.0
38	61622.0
39	71302.0
40	93867.5
41	116433.0
42	151854.5
43	187276.0
44	189509.5
45	191743.0
46	182834.5
47	173926.0
48	164341.0
49	154756.0
50	135557.0
51	116358.0
52	93510.0
53	70662.0
54	57788.5
55	44915.0
56	36053.5
57	27192.0
58	22464.0
59	17736.0
60	14044.5
61	10353.0
62	8739.5
63	7126.0
64	5317.5
65	3509.0
66	2696.0
67	1883.0
68	1357.5
69	832.0
70	696.5
71	561.0
72	425.5
73	290.0
74	270.0
75	197.5
76	145.0
77	157.5
78	170.0
79	128.0
80	86.0
81	57.5
82	29.0
83	20.5
84	12.0
85	9.0
86	6.0
87	4.5
88	3.0
89	1.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1511693.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.734878519067212
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.90451625743381	20.827677782291897
2	9.535940954101857	5.0988444594582205
3	3.6335275629542765	2.914257539737948
4	1.8703168523568248	2.0001077535969554
5	1.1622893974390365	1.5536832922266237
6	0.8040193152603906	1.2897215232282089
7	0.5877789245147247	1.09999387000784
8	0.47126820035265926	1.0079438469062219
9	0.35036606625301797	0.84302947966301
>10	2.2495759650328724	12.136812691803915
>50	0.5862613332352624	11.45862489272661
>100	0.8358608374431271	37.09427885579555
>500	0.006271464865324529	1.0481190209156328
>1k	0.0017560101622908682	0.8137878295233097
>5k	0.0	0.0
>10k+	2.5085859461298116E-4	0.8131171621180657
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12124	0.8020146947826048	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	4527	0.2994655660904694	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1635	0.10815688105984482	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.615099759011916E-5	0.0	0.0	0.08659165584546598	0.0
2	6.615099759011916E-5	0.0	0.0	0.3168632784566708	0.0
3	6.615099759011916E-5	0.0	0.0	0.5010276557475625	0.0
4	6.615099759011916E-5	0.0	0.0	0.9368304278712675	0.0
5	6.615099759011916E-5	0.0	0.0	1.6272483897193413	0.0
6	6.615099759011916E-5	0.0	0.0	2.4806624096294683	0.0
7	6.615099759011916E-5	0.0	0.0	2.898405959411071	0.0
8	6.615099759011916E-5	0.0	0.0	4.0273388842840445	0.0
9	6.615099759011916E-5	0.0	0.0	4.365899689950274	0.0
10	6.615099759011916E-5	0.0	0.0	5.040309110381539	0.0
11	6.615099759011916E-5	0.0	0.0	5.89021712741939	0.0
12	6.615099759011916E-5	0.0	0.0	6.525200553286944	0.0
13	6.615099759011916E-5	0.0	0.0	6.8102452019027675	0.0
14	6.615099759011916E-5	0.0	0.0	6.907487168360243	0.0
15	1.3230199518023833E-4	0.0	0.0	7.059436009824746	0.0
16	1.3230199518023833E-4	0.0	0.0	7.464809323056996	0.0
17	1.3230199518023833E-4	0.0	0.0	7.939310428770921	0.0
18	1.3230199518023833E-4	0.0	0.0	8.513038030870025	0.0
19	1.3230199518023833E-4	0.0	0.0	8.793253656661769	0.0
20	1.3230199518023833E-4	0.0	0.0	9.0929838267426	0.0
21	1.3230199518023833E-4	0.0	0.0	9.52693437093378	0.0
22	1.3230199518023833E-4	0.0	0.0	9.967896920869515	0.0
23	1.3230199518023833E-4	0.0	0.0	10.530378853378298	0.0
24	1.3230199518023833E-4	0.0	0.0	10.89176175321312	0.0
25	1.3230199518023833E-4	0.0	0.0	11.206309746754135	0.0
26	1.3230199518023833E-4	0.0	0.0	11.490031375418157	0.0
27	1.3230199518023833E-4	0.0	0.0	11.75860442563404	0.0
28	1.3230199518023833E-4	0.0	0.0	12.075533855088302	0.0
29	1.3230199518023833E-4	0.0	0.0	12.41343315077863	0.0
30	1.3230199518023833E-4	0.0	0.0	12.789567723076049	0.0
31	1.3230199518023833E-4	0.0	0.0	13.160608668559027	0.0
32	1.3230199518023833E-4	0.0	0.0	13.494671206389128	0.0
33	1.3230199518023833E-4	0.0	0.0	13.816429658667468	0.0
34	1.3230199518023833E-4	0.0	0.0	14.141627962820493	0.0
35	1.3230199518023833E-4	0.0	0.0	14.582855116746588	0.0
36	1.3230199518023833E-4	0.0	0.0	14.924988076282684	0.0
37	1.3230199518023833E-4	0.0	0.0	15.270031679712746	0.0
38	1.3230199518023833E-4	0.0	0.0	15.598868288733229	0.0
39	1.3230199518023833E-4	0.0	0.0	15.920560590013977	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCCATC	50	2.1827873E-11	45.000004	9
TCGTTAG	55	1.8189894E-12	45.000004	1
CAACGAC	25	3.891391E-5	45.000004	1
TCCGATC	25	3.891391E-5	45.000004	23
CAATTCG	25	3.891391E-5	45.000004	18
CGCTAGG	55	1.8189894E-12	45.000004	2
GTCCGAT	25	3.891391E-5	45.000004	22
TCCAACG	25	3.891391E-5	45.000004	1
TACTAAG	25	3.891391E-5	45.000004	1
TAGTGCG	75	0.0	45.000004	1
TGTCGCG	25	3.891391E-5	45.000004	1
ATCGACC	25	3.891391E-5	45.000004	21
TACGTAG	100	0.0	45.000004	1
ACGCGAG	45	3.8562575E-10	45.0	1
CAACGCC	20	7.033994E-4	45.0	17
GCGCGTC	40	6.8175723E-9	45.0	38
TGGACGC	20	7.033994E-4	45.0	27
CATTGCG	20	7.033994E-4	45.0	1
TAACGCG	40	6.8175723E-9	45.0	1
CTGCGCG	20	7.033994E-4	45.0	1
>>END_MODULE