##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547633_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1340538
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.424950281155773	31.0	31.0	33.0	30.0	34.0
2	31.832348654047852	31.0	31.0	34.0	30.0	34.0
3	31.962405392461832	33.0	31.0	34.0	30.0	34.0
4	35.7044738754142	37.0	35.0	37.0	33.0	37.0
5	35.625359370640744	37.0	35.0	37.0	33.0	37.0
6	35.67884759700956	37.0	35.0	37.0	33.0	37.0
7	35.993862911756324	37.0	35.0	37.0	35.0	37.0
8	36.034779319944676	37.0	35.0	37.0	35.0	37.0
9	37.892214916697625	39.0	38.0	39.0	35.0	39.0
10	37.32728874526496	39.0	37.0	39.0	34.0	39.0
11	36.93818451994647	39.0	37.0	39.0	33.0	39.0
12	36.02290796680139	37.0	35.0	39.0	32.0	39.0
13	35.68490412058442	37.0	35.0	39.0	31.0	39.0
14	36.62935328950019	38.0	35.0	41.0	31.0	41.0
15	36.97210149954719	38.0	35.0	41.0	32.0	41.0
16	37.130786296248225	38.0	35.0	41.0	33.0	41.0
17	37.03986608361718	38.0	35.0	41.0	32.0	41.0
18	37.00343220408523	38.0	35.0	41.0	32.0	41.0
19	36.92452433276789	38.0	35.0	41.0	32.0	41.0
20	36.74209608381113	38.0	35.0	40.0	32.0	41.0
21	36.56599514523273	38.0	35.0	40.0	32.0	41.0
22	36.48518803644507	38.0	35.0	40.0	31.0	41.0
23	36.47239690333284	38.0	35.0	40.0	31.0	41.0
24	36.41568758214985	37.0	35.0	40.0	31.0	41.0
25	36.30008772597271	37.0	35.0	40.0	31.0	41.0
26	36.19873737260712	37.0	35.0	40.0	31.0	41.0
27	36.15493779363211	37.0	35.0	40.0	31.0	41.0
28	36.20576216414604	37.0	35.0	40.0	31.0	41.0
29	36.28480356394224	37.0	35.0	40.0	31.0	41.0
30	36.31850645039529	37.0	35.0	40.0	31.0	41.0
31	36.182544620145045	37.0	35.0	40.0	31.0	41.0
32	35.997745681211576	37.0	35.0	40.0	30.0	41.0
33	35.892995200434456	37.0	35.0	40.0	30.0	41.0
34	35.822418312647606	37.0	35.0	40.0	30.0	41.0
35	35.68535393998529	37.0	35.0	40.0	30.0	41.0
36	35.526438638815165	37.0	35.0	40.0	29.0	41.0
37	35.47944258200812	37.0	34.0	40.0	29.0	41.0
38	35.439178896830974	37.0	34.0	40.0	29.0	41.0
39	35.429440269503736	37.0	34.0	40.0	29.0	41.0
40	35.319891715117365	37.0	34.0	40.0	29.0	41.0
41	35.28940917750933	37.0	34.0	40.0	29.0	41.0
42	35.25625457838569	36.0	34.0	40.0	29.0	41.0
43	35.2150897624685	36.0	34.0	40.0	29.0	41.0
44	35.08929325390254	36.0	34.0	40.0	28.0	41.0
45	34.99939352707644	36.0	34.0	40.0	27.0	41.0
46	34.987275258142624	36.0	34.0	40.0	28.0	41.0
47	34.9559579810494	36.0	34.0	40.0	28.0	41.0
48	34.87443474187229	36.0	34.0	40.0	27.0	41.0
49	34.8342829520685	36.0	34.0	40.0	27.0	41.0
50	34.75117154455898	36.0	34.0	40.0	27.0	41.0
51	34.30872679476449	35.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	3.0
13	7.0
14	1.0
15	14.0
16	42.0
17	129.0
18	364.0
19	738.0
20	1331.0
21	2205.0
22	3671.0
23	5427.0
24	8378.0
25	12612.0
26	17158.0
27	20950.0
28	22892.0
29	26426.0
30	31542.0
31	39396.0
32	50405.0
33	68776.0
34	127420.0
35	222375.0
36	85628.0
37	106551.0
38	166631.0
39	319320.0
40	145.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.07644990294941	24.345598558190815	30.272323499967925	13.305628038891848
2	29.156652030751832	26.37015884667201	31.345698518057674	13.127490604518485
3	28.70608666072875	25.661040567294624	32.36290205872567	13.269970713250947
4	25.184291679907616	28.040980561535743	31.996631203293006	14.778096555263634
5	23.526524425268065	33.14497612152733	29.60639683470368	13.72210261850093
6	21.551272697976483	41.00182165667814	26.863319055483693	10.583586589861682
7	89.28654018013663	3.657934351730425	5.80281946502076	1.2527060031121833
8	90.66591174588113	2.469829277499034	5.299514075691998	1.5647449009278365
9	86.16488305441547	4.9182492402304145	6.865377930353336	2.0514897750007832
10	51.11417953090476	29.425499314454346	11.40415266109577	8.05616849354513
11	43.39183223452076	19.46733326470417	24.057952851765485	13.082881649009575
12	40.9231965076708	21.426322864402202	23.825135878281706	13.825344749645291
13	21.578276781411642	39.69906112322067	24.243027799286555	14.479634296081127
14	13.895465850278022	42.24497925459778	27.74691952037167	16.112635374752525
15	13.059682008268322	26.183144379346203	46.63746943391385	14.119704178471629
16	16.04169370804856	20.835142308535826	45.70836485053016	17.414799132885452
17	15.760239545615267	21.988559817028687	29.200664210936207	33.05053642641984
18	20.575619639279154	25.071053562077317	34.80386232990038	19.549464468743146
19	27.588624865539057	25.675885353492404	27.38803375958011	19.347456021388428
20	30.005639526816847	24.559467915120646	27.53857033519378	17.89632222286873
21	21.954543623530252	28.92793788762422	29.37141655066846	19.74610193817706
22	22.211977579151057	24.10606786230603	27.643602792311743	26.038351766231173
23	18.291312890794593	30.002208068700774	26.92053488972338	24.78594415078125
24	19.61951097246031	24.125090075775546	38.91407778071193	17.34132117105222
25	16.631829907097	26.809683873191208	35.38191382862701	21.176572391084775
26	15.766431089607307	36.57807536973961	27.854935854112306	19.800557686540778
27	16.651374298975487	36.6435714616072	29.769391095216996	16.935663144200312
28	14.279789159277842	30.2147346811504	39.461022365647224	16.04445379392453
29	15.074619294641407	24.79146432253319	38.99561220942637	21.13830417339904
30	17.381454311627124	32.25406515891381	33.23053878368237	17.133941745776696
31	28.47461243172517	28.1406420407329	26.36821932686727	17.016526200674654
32	27.792796623445216	27.628758006113962	28.19092036182488	16.387525008615945
33	26.5027175656341	29.372684698233098	25.636647375904303	18.487950360228506
34	18.53009761752371	29.18089602831102	29.2701885362444	23.018817817920866
35	19.907753454210177	27.401908785875523	31.214482543575787	21.475855216338516
36	28.278497140700225	25.91929508898666	29.296894232017294	16.50531353829582
37	19.495381704957264	32.48598696941079	30.53803771321663	17.480593612415312
38	18.313318980886777	34.25691774496508	26.0730393319697	21.356723942178437
39	19.49224863450346	32.41086787543509	29.048113518602232	19.04876997145922
40	23.87869646365862	27.622044283712953	26.722256288146994	21.777002964481422
41	16.931784104590843	25.5971856075695	30.219583480662244	27.251446807177416
42	21.383504234866894	24.792956260844527	27.116500986917192	26.707038517371384
43	22.11955200076387	25.85760343981297	27.43003182304418	24.592812736378978
44	18.126229916645407	31.64147528827978	28.854907507284388	21.377387287790427
45	17.174820855507267	38.321256092703074	24.35895140607726	20.144971645712392
46	21.977967055018212	34.03924394534135	25.77330892522256	18.209480074417883
47	20.552718386200166	29.180597640648752	27.538271947531513	22.728412025619566
48	22.379969833007348	24.98392436469537	32.329035059058384	20.30707074323891
49	20.14638898710816	24.743050924330383	33.351161996153785	21.759398092407675
50	18.616779233412256	32.56968470867667	29.688155054164824	19.125381003746256
51	17.685362145646003	33.517438520952034	26.404249637086007	22.392949696315956
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	735.0
1	1299.0
2	1863.0
3	6676.0
4	11489.0
5	8143.5
6	4798.0
7	4927.0
8	5056.0
9	5533.0
10	6010.0
11	6112.5
12	6215.0
13	6006.5
14	5798.0
15	5656.0
16	5514.0
17	5033.5
18	4553.0
19	4424.0
20	4295.0
21	4544.0
22	4793.0
23	5376.5
24	5960.0
25	7261.5
26	11020.5
27	13478.0
28	16820.5
29	20163.0
30	22243.5
31	24324.0
32	29460.0
33	34596.0
34	40289.0
35	45982.0
36	47207.0
37	48432.0
38	59244.5
39	70057.0
40	87729.5
41	105402.0
42	128240.0
43	151078.0
44	150700.5
45	150323.0
46	144002.0
47	137681.0
48	126749.5
49	115818.0
50	104523.5
51	93229.0
52	81682.5
53	70136.0
54	61156.0
55	52176.0
56	45099.5
57	38023.0
58	33503.5
59	28984.0
60	26107.5
61	23231.0
62	19645.5
63	16060.0
64	13090.0
65	10120.0
66	8319.0
67	6518.0
68	5094.5
69	3671.0
70	2987.0
71	2303.0
72	1904.0
73	1505.0
74	1140.5
75	696.5
76	617.0
77	365.5
78	114.0
79	96.5
80	79.0
81	46.5
82	14.0
83	9.0
84	4.0
85	2.5
86	1.0
87	0.5
88	0.0
89	0.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1340538.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.96389982231179
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.3856996529014	13.297271564136395
2	7.937258228463823	2.6929370690296053
3	2.548286431871423	1.2968662724646955
4	1.2599300428546238	0.8549330812042738
5	0.6992447127342725	0.5930958629052692
6	0.5084181698195148	0.5174852940396812
7	0.38979963360113445	0.462876535462844
8	0.28307956445646115	0.3841706698546448
9	0.27662651557675944	0.4223398048595387
>10	4.197409692310991	18.53959127898025
>50	2.0652427841672654	25.38546988632515
>100	1.4414460938839322	34.00088212872424
>500	0.006669244728025597	0.7342552324740302
>1k	4.446163152017064E-4	0.2520678979895438
>5k	4.446163152017064E-4	0.5657574215498409
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7501	0.5595514636660803	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3342	0.24930289182402884	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.047443638300443555	0.0
2	0.0	0.0	0.0	0.1772422713865627	0.0
3	0.0	0.0	0.0	0.26071621990573934	0.0
4	0.0	0.0	0.0	0.49316020881168604	0.0
5	0.0	0.0	0.0	0.7928160186432611	0.0
6	0.0	0.0	0.0	1.1501352442079225	0.0
7	0.0	0.0	0.0	1.3146214430325736	0.0
8	0.0	0.0	0.0	1.7943542070422471	0.0
9	0.0	0.0	0.0	1.934969392885543	0.0
10	0.0	0.0	0.0	2.199415458569619	0.0
11	0.0	0.0	0.0	2.5315955235882908	0.0
12	0.0	0.0	0.0	2.802083939433272	0.0
13	0.0	0.0	0.0	2.9098018855116377	0.0
14	0.0	0.0	0.0	2.9535902749493115	0.0
15	0.0	0.0	0.0	3.019981529803706	0.0
16	0.0	0.0	0.0	3.171637059150878	0.0
17	0.0	0.0	0.0	3.3678269470913915	0.0
18	0.0	0.0	0.0	3.607954418300712	0.0
19	0.0	0.0	0.0	3.7378276483023978	0.0
20	0.0	0.0	0.0	3.87806984956786	0.0
21	0.0	0.0	0.0	4.061429068030895	0.0
22	0.0	0.0	0.0	4.26582461668375	0.0
23	0.0	0.0	0.0	4.479693973613579	0.0
24	0.0	0.0	0.0	4.64052492357546	0.0
25	0.0	0.0	0.0	4.778753008120621	0.0
26	0.0	0.0	0.0	4.9102673702647746	0.0
27	0.0	0.0	0.0	5.047600291823134	0.0
28	0.0	0.0	0.0	5.189334431399931	0.0
29	0.0	0.0	0.0	5.346659326330175	0.0
30	0.0	0.0	0.0	5.545460106315524	0.0
31	0.0	0.0	0.0	5.731131829161128	0.0
32	0.0	0.0	0.0	5.8890534994159065	0.0
33	0.0	0.0	0.0	6.04936227096882	0.0
34	0.0	0.0	0.0	6.225038007128481	0.0
35	0.0	0.0	0.0	6.444502132725816	0.0
36	0.0	0.0	0.0	6.6324863599539885	0.0
37	0.0	0.0	0.0	6.839641994482812	0.0
38	0.0	0.0	0.0	7.019420560998644	0.0
39	0.0	0.0	0.0	7.229560072150137	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTAGCG	55	1.8189894E-12	45.000004	1
CGAGTTA	30	2.1657725E-6	45.000004	37
TTTCGCG	25	3.8911538E-5	45.000004	1
GGCGTGA	25	3.8911538E-5	45.000004	8
CCGAGTT	30	2.1657725E-6	45.000004	36
TCCACGA	30	2.1657725E-6	45.000004	23
TCGTAAG	60	0.0	45.000004	1
ACCGTTC	30	2.1657725E-6	45.000004	27
CGCACTC	25	3.8911538E-5	45.000004	12
CGCGTCC	25	3.8911538E-5	45.000004	23
CCGCAAT	50	2.1827873E-11	45.000004	33
TTGATCG	20	7.033707E-4	45.0	20
CGGGTAC	20	7.033707E-4	45.0	6
CTATGCG	20	7.033707E-4	45.0	1
GTCGACG	20	7.033707E-4	45.0	13
CGCGGAT	35	1.2121382E-7	45.0	20
ATAGCGC	20	7.033707E-4	45.0	12
GCACGAG	20	7.033707E-4	45.0	1
AGCGGTG	20	7.033707E-4	45.0	41
AAACGCG	20	7.033707E-4	45.0	1
>>END_MODULE