FastQCFastQC Report
Thu 26 May 2016
SRR1547631_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547631_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1617043
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83450.5160654354893469No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC40550.2507663679939247No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT37050.22912192192786462No Hit
GCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGC17390.1075419763110814RNA PCR Primer, Index 35 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGATAT700.045.0000049
ACAACGC253.891512E-545.040
CGTATCG207.034139E-445.037
CCTAGCG207.034139E-445.01
GCGCGAA207.034139E-445.018
CGGTTCA207.034139E-445.041
CACGCAT253.891512E-545.043
CGCGATT207.034139E-445.043
CGCGATA207.034139E-445.011
CCCACGT207.034139E-445.029
AACGGTC207.034139E-445.025
TACGCAA207.034139E-445.01
CGAATTC207.034139E-445.023
GCTAGCG406.8175723E-945.01
ACGTACG207.034139E-445.038
ACCAACG207.034139E-445.031
CCGCGAT207.034139E-445.010
GGTCTCG207.034139E-445.010
CGCGTCA207.034139E-445.039
TTCGCGA253.891512E-545.01