##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547631_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1617043
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.168188477362694	31.0	31.0	33.0	30.0	34.0
2	31.550375592980522	31.0	31.0	34.0	30.0	34.0
3	31.66433421993107	31.0	31.0	34.0	30.0	34.0
4	35.445549067031614	37.0	35.0	37.0	33.0	37.0
5	35.34126736271082	37.0	35.0	37.0	33.0	37.0
6	35.38897295866591	37.0	35.0	37.0	33.0	37.0
7	35.813710581598635	37.0	35.0	37.0	35.0	37.0
8	35.833608630073535	37.0	35.0	37.0	35.0	37.0
9	37.651863308520554	39.0	37.0	39.0	35.0	39.0
10	36.94257852141223	39.0	37.0	39.0	32.0	39.0
11	36.6320945083093	39.0	35.0	39.0	32.0	39.0
12	35.76336065274702	37.0	35.0	39.0	31.0	39.0
13	35.4264994808425	37.0	35.0	39.0	30.0	39.0
14	36.347825011456095	38.0	35.0	40.0	31.0	41.0
15	36.642365725586764	38.0	35.0	41.0	31.0	41.0
16	36.806712622979106	38.0	35.0	41.0	32.0	41.0
17	36.72229866490873	38.0	35.0	40.0	32.0	41.0
18	36.65370927056362	38.0	35.0	40.0	31.0	41.0
19	36.56939364011965	37.0	35.0	40.0	31.0	41.0
20	36.379092578243124	37.0	35.0	40.0	31.0	41.0
21	36.187912751856324	37.0	35.0	40.0	31.0	41.0
22	36.09615205037838	37.0	35.0	40.0	30.0	41.0
23	36.07655455049742	37.0	35.0	40.0	31.0	41.0
24	35.96807877094177	37.0	34.0	40.0	30.0	41.0
25	35.87444366043451	37.0	34.0	40.0	30.0	41.0
26	35.72368947517165	36.0	34.0	40.0	30.0	41.0
27	35.65682483397164	36.0	34.0	40.0	30.0	41.0
28	35.686845062252516	36.0	34.0	40.0	30.0	41.0
29	35.71973287043078	36.0	34.0	40.0	30.0	41.0
30	35.77737512236842	36.0	35.0	40.0	30.0	41.0
31	35.61624891855071	36.0	34.0	40.0	30.0	41.0
32	35.44356148847	36.0	34.0	40.0	29.0	41.0
33	35.3199191363495	37.0	34.0	40.0	29.0	41.0
34	35.21048172497577	36.0	34.0	40.0	28.0	41.0
35	35.08674970300728	36.0	34.0	40.0	27.0	41.0
36	34.97008366506024	36.0	34.0	40.0	27.0	41.0
37	34.94030276251157	36.0	34.0	40.0	27.0	41.0
38	34.88365739191846	36.0	34.0	40.0	27.0	41.0
39	34.84639060309466	36.0	34.0	40.0	26.0	41.0
40	34.73736381778345	36.0	34.0	40.0	25.0	41.0
41	34.68833172649089	36.0	34.0	40.0	25.0	41.0
42	34.630026536090874	36.0	34.0	40.0	25.0	41.0
43	34.55720905380995	36.0	33.0	40.0	25.0	41.0
44	34.43380479059617	36.0	34.0	40.0	24.0	41.0
45	34.34807299496674	35.0	33.0	40.0	24.0	41.0
46	34.29834642616183	35.0	33.0	40.0	24.0	41.0
47	34.239447559526866	35.0	33.0	40.0	24.0	41.0
48	34.172631772933684	35.0	33.0	40.0	23.0	41.0
49	34.174247067022954	36.0	33.0	40.0	24.0	41.0
50	34.055253941917435	35.0	33.0	40.0	24.0	41.0
51	33.61076607115581	35.0	33.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	4.0
11	3.0
12	3.0
13	4.0
14	5.0
15	27.0
16	92.0
17	284.0
18	699.0
19	1364.0
20	2450.0
21	4052.0
22	6140.0
23	9378.0
24	14021.0
25	19976.0
26	26772.0
27	31320.0
28	34388.0
29	38819.0
30	45912.0
31	56516.0
32	69701.0
33	89873.0
34	153358.0
35	252369.0
36	109264.0
37	130739.0
38	188697.0
39	330683.0
40	130.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.67371368603062	24.681656579324112	29.425438902985263	13.219190831660011
2	29.446959666502377	26.656186632019065	30.70883087215368	13.188022829324886
3	27.586403082663853	25.35937510628969	33.5613214985625	13.492900312483961
4	24.784189412402764	27.8402615143815	32.43451163636341	14.941037436852328
5	21.892244052879235	32.55881259805707	30.999608544732578	14.549334804331115
6	20.631857037815323	39.955647437946915	28.74778221729416	10.6647133069436
7	86.94394645040362	3.5832071255990097	7.701836005597872	1.7710104183995108
8	86.39541434581517	3.5916793802020104	6.995175762178247	3.017730511804572
9	82.14537275755809	5.117118097663451	9.43895740558538	3.2985517391930825
10	47.46132292091181	24.99129584061772	15.556481800422128	11.990899438048338
11	40.92482389151061	21.041741005032026	23.91049588662763	14.122939216829733
12	39.23779392384742	20.278310471644847	25.025926954323417	15.457968650184316
13	20.476944645256808	40.058551318672414	25.229755794991227	14.23474824107955
14	14.982965820946012	41.25592207504686	27.029027675825567	16.73208442818156
15	13.431306403107401	26.032641061493106	46.184919015759014	14.35113351964048
16	14.053058576673594	22.008876696538064	44.95495790773653	18.983106819051812
17	14.90554054530399	24.258971468291197	28.330539138414995	32.504948847989816
18	20.3065100928052	25.229508429893333	34.61757046658624	19.846411010715237
19	25.58608521851305	29.396064297609897	25.384668187549742	19.63318229632731
20	28.342474504388566	24.946955646819536	27.694439789170726	19.016130059621172
21	19.35780310109255	31.53688553736666	28.840049398810052	20.265261962730737
22	20.49141550348383	25.429070222622403	27.00509510260395	27.074419171289815
23	17.40077412907387	31.05198810421244	26.467818109969866	25.079419656743823
24	18.933757482021196	25.544775247164115	38.18154495582369	17.339922314991004
25	15.52624141720412	28.226212908376585	34.17880662418995	22.06873905022934
26	14.814881236924435	36.35803129539536	28.32503526498677	20.502052202693434
27	16.170627497227965	35.78488636356609	29.261868732000323	18.78261740720562
28	13.716332837160175	29.599707614454285	38.27251347057562	18.411446077809927
29	13.565811175089346	24.222732481449164	38.93804926646972	23.273407076991766
30	16.400615196998473	32.17830323621573	32.68268067082941	18.738400895956385
31	25.65571849357129	28.444017877075623	25.883170701088343	20.01709292826474
32	24.856234497165506	27.876067612302208	27.83982862546018	19.427869265072108
33	23.836719246179598	29.284688162281398	26.74715514677099	20.13143744476801
34	15.684616921133204	28.307657866859447	28.881792259080306	27.12593295292704
35	17.13460928373581	26.19621123247805	32.575447900890694	24.093731582895444
36	24.962539647987096	25.32288875434976	29.64219257001824	20.072379027644903
37	16.340010748013505	31.261382659582953	32.085665007053	20.312941585350544
38	15.984979991255644	32.47105982957781	28.22336820975076	23.32059196941578
39	17.34319990253815	31.12953706240341	30.761457796731438	20.765805238326994
40	21.963608883622758	27.149989208697605	27.754487666685424	23.13191424099421
41	14.722119325212748	24.284882962295992	31.68796377090776	29.305033941583496
42	19.910045682149455	24.07493183545521	27.332668333495153	28.682354148900185
43	20.122408618694738	25.6634486528806	28.271666245115313	25.942476483309353
44	16.858859040854203	30.772465543587895	28.849140066157798	23.51953534940011
45	15.37806972356332	37.29338057182153	24.73644794850848	22.592101756106672
46	20.888189120511946	33.21457747258422	25.4980850849359	20.39914832196794
47	17.771821775920614	28.07643334159945	29.620362600128754	24.53138228235118
48	19.87516720334586	24.516231170104938	33.430279837951126	22.178321788598076
49	18.706305274504142	23.690588314596457	32.74192461177594	24.86118179912346
50	16.798192750594758	30.53406743048886	30.781865417308012	21.88587440160837
51	14.978760614281747	32.12202767644398	27.27175467813781	25.627457031136462
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	866.0
1	1291.0
2	1716.0
3	7458.5
4	13201.0
5	9731.0
6	6261.0
7	6435.0
8	6609.0
9	7323.5
10	8038.0
11	8203.0
12	8368.0
13	8349.0
14	8330.0
15	8065.5
16	7801.0
17	7485.0
18	7169.0
19	7219.5
20	7270.0
21	7392.0
22	7514.0
23	7178.0
24	6842.0
25	8610.0
26	12945.0
27	15512.0
28	19177.5
29	22843.0
30	25975.5
31	29108.0
32	35123.0
33	41138.0
34	45983.0
35	50828.0
36	52201.5
37	53575.0
38	64783.0
39	75991.0
40	99775.0
41	123559.0
42	147444.0
43	171329.0
44	179701.0
45	188073.0
46	184654.5
47	181236.0
48	171649.0
49	162062.0
50	143571.0
51	125080.0
52	105470.0
53	85860.0
54	72800.5
55	59741.0
56	50536.5
57	41332.0
58	36054.5
59	30777.0
60	26051.0
61	21325.0
62	17621.5
63	13918.0
64	11327.5
65	8737.0
66	7281.0
67	5825.0
68	4718.5
69	3612.0
70	2959.0
71	2306.0
72	1910.0
73	1514.0
74	1103.0
75	498.5
76	305.0
77	278.5
78	252.0
79	165.0
80	78.0
81	56.0
82	34.0
83	19.5
84	5.0
85	7.5
86	10.0
87	12.5
88	15.0
89	10.0
90	5.0
91	2.5
92	0.0
93	0.5
94	1.0
95	1.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1617043.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.907129415565002
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.75403336776073	18.588757383052155
2	9.604891973652888	4.5925079087328236
3	3.5215161464511833	2.5256802675663064
4	1.6431616119877042	1.5713310923391381
5	0.9543406842911795	1.1407773122944047
6	0.6369738006545718	0.9136929051943892
7	0.4575733874041194	0.765748633285212
8	0.3626094524539376	0.6935160885698761
9	0.28394157395903025	0.6109405159587982
>10	2.8239577148388975	15.613884979309239
>50	1.0191209797505985	17.669104986170286
>100	0.9302798376996811	32.863989046110916
>500	0.004192810535442585	0.6393935512871073
>1k	0.003144607901581939	1.2878711771319873
>5k	2.620506584651616E-4	0.5228041529973457
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8345	0.5160654354893469	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	4055	0.2507663679939247	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	3705	0.22912192192786462	No Hit
GCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGC	1739	0.1075419763110814	RNA PCR Primer, Index 35 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.184127447445738E-5	0.0	0.0	0.10383149984261396	0.0
2	6.184127447445738E-5	0.0	0.0	0.3869408543866799	0.0
3	6.184127447445738E-5	0.0	0.0	0.5882960440755132	0.0
4	6.184127447445738E-5	0.0	0.0	1.083953858988289	0.0
5	6.184127447445738E-5	0.0	0.0	1.9233254774300992	0.0
6	6.184127447445738E-5	0.0	0.0	2.845935451314529	0.0
7	6.184127447445738E-5	0.0	0.0	3.274248118324621	0.0
8	6.184127447445738E-5	0.0	0.0	4.497035638508067	0.0
9	6.184127447445738E-5	0.0	0.0	4.853179538206467	0.0
10	6.184127447445738E-5	0.0	0.0	5.612652230027278	0.0
11	6.184127447445738E-5	0.0	0.0	6.454064610526745	0.0
12	6.184127447445738E-5	0.0	0.0	7.1260318989661995	0.0
13	6.184127447445738E-5	0.0	0.0	7.41087280919555	0.0
14	6.184127447445738E-5	0.0	0.0	7.5192187220747995	0.0
15	6.184127447445738E-5	0.0	0.0	7.686066480606885	0.0
16	6.184127447445738E-5	0.0	0.0	8.090755780767735	0.0
17	6.184127447445738E-5	0.0	0.0	8.573488769315349	0.0
18	6.184127447445738E-5	0.0	0.0	9.159063797313985	0.0
19	6.184127447445738E-5	0.0	0.0	9.479772646738523	0.0
20	6.184127447445738E-5	0.0	0.0	9.791514511364262	0.0
21	6.184127447445738E-5	0.0	0.0	10.262064768840409	0.0
22	6.184127447445738E-5	0.0	0.0	10.743993820819854	0.0
23	6.184127447445738E-5	0.0	0.0	11.334083261855127	0.0
24	6.184127447445738E-5	0.0	0.0	11.71607681428385	0.0
25	6.184127447445738E-5	0.0	0.0	12.069004967709578	0.0
26	6.184127447445738E-5	0.0	0.0	12.375985054200784	0.0
27	6.184127447445738E-5	0.0	0.0	12.696013649606101	0.0
28	6.184127447445738E-5	0.0	0.0	13.039418246762764	0.0
29	6.184127447445738E-5	0.0	0.0	13.40242652792783	0.0
30	6.184127447445738E-5	0.0	0.0	13.798334367113306	0.0
31	6.184127447445738E-5	0.0	0.0	14.192943539534818	0.0
32	6.184127447445738E-5	0.0	0.0	14.546675629528714	0.0
33	6.184127447445738E-5	0.0	0.0	14.905416862755041	0.0
34	6.184127447445738E-5	0.0	0.0	15.25723187324023	0.0
35	6.184127447445738E-5	0.0	0.0	15.696675969655724	0.0
36	6.184127447445738E-5	0.0	0.0	16.0669196799343	0.0
37	6.184127447445738E-5	0.0	0.0	16.466290630490345	0.0
38	6.184127447445738E-5	0.0	0.0	16.84426450007823	0.0
39	6.184127447445738E-5	0.0	0.0	17.209808273496748	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGATAT	70	0.0	45.000004	9
ACAACGC	25	3.891512E-5	45.0	40
CGTATCG	20	7.034139E-4	45.0	37
CCTAGCG	20	7.034139E-4	45.0	1
GCGCGAA	20	7.034139E-4	45.0	18
CGGTTCA	20	7.034139E-4	45.0	41
CACGCAT	25	3.891512E-5	45.0	43
CGCGATT	20	7.034139E-4	45.0	43
CGCGATA	20	7.034139E-4	45.0	11
CCCACGT	20	7.034139E-4	45.0	29
AACGGTC	20	7.034139E-4	45.0	25
TACGCAA	20	7.034139E-4	45.0	1
CGAATTC	20	7.034139E-4	45.0	23
GCTAGCG	40	6.8175723E-9	45.0	1
ACGTACG	20	7.034139E-4	45.0	38
ACCAACG	20	7.034139E-4	45.0	31
CCGCGAT	20	7.034139E-4	45.0	10
GGTCTCG	20	7.034139E-4	45.0	10
CGCGTCA	20	7.034139E-4	45.0	39
TTCGCGA	25	3.891512E-5	45.0	1
>>END_MODULE