FastQCFastQC Report
Thu 26 May 2016
SRR1547630_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547630_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2013658
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108650.5395653085081975No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC28420.14113618101981568No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGGAT253.8918537E-545.00000410
CACCGCA253.8918537E-545.00000428
GCGCTAA253.8918537E-545.00000436
TCGACTC253.8918537E-545.00000414
CGAGGTA207.034551E-445.013
GCGATAC351.2125383E-745.09
TACTCGC406.8193913E-945.045
CTACGCG600.044.9999961
GCGATCG302.1663182E-644.9999969
CGTTTTT71450.041.4100761
CGTAAGG2800.040.982142
CGCATCG556.184564E-1140.90909221
CCGCTCG501.0822987E-940.50000419
GGCGATA6350.040.39378
TCGTAAG900.040.0000041
TTAGTCG451.929584E-840.00000415
TGTAGCG1250.039.61
GTTAGCG1150.039.1304321
TACGCGG1150.039.1304322
CGATGCG750.039.010
Produced by FastQC (version 0.11.5)