##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547630_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2013658
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.376512794128892	31.0	31.0	33.0	30.0	34.0
2	31.785430793113825	31.0	31.0	34.0	30.0	34.0
3	31.899513720800652	31.0	31.0	34.0	30.0	34.0
4	35.66834834912383	37.0	35.0	37.0	33.0	37.0
5	35.58068996820711	37.0	35.0	37.0	33.0	37.0
6	35.631366895470826	37.0	35.0	37.0	33.0	37.0
7	35.951204226338334	37.0	35.0	37.0	35.0	37.0
8	35.9863522008206	37.0	35.0	37.0	35.0	37.0
9	37.81210215438769	39.0	38.0	39.0	35.0	39.0
10	37.20804724536143	39.0	37.0	39.0	33.0	39.0
11	36.910585610863414	39.0	37.0	39.0	33.0	39.0
12	36.22612330395727	38.0	35.0	39.0	32.0	39.0
13	35.980391407081044	38.0	35.0	39.0	32.0	39.0
14	36.884425756508804	39.0	35.0	41.0	31.0	41.0
15	37.15139263966373	39.0	35.0	41.0	32.0	41.0
16	37.28289659912458	39.0	35.0	41.0	32.0	41.0
17	37.22296189323112	39.0	35.0	41.0	32.0	41.0
18	37.116322136132354	38.0	35.0	41.0	32.0	41.0
19	37.07450123109287	38.0	35.0	41.0	32.0	41.0
20	36.91401419704836	38.0	35.0	41.0	32.0	41.0
21	36.73862939982857	38.0	35.0	41.0	31.0	41.0
22	36.70813812474611	38.0	35.0	41.0	31.0	41.0
23	36.66782839985738	38.0	35.0	40.0	31.0	41.0
24	36.57651597242432	38.0	35.0	40.0	31.0	41.0
25	36.45994503535357	38.0	35.0	40.0	31.0	41.0
26	36.37115835956255	38.0	35.0	40.0	31.0	41.0
27	36.30860404299042	38.0	35.0	40.0	31.0	41.0
28	36.33410142139331	38.0	35.0	40.0	31.0	41.0
29	36.35510449142804	38.0	35.0	40.0	31.0	41.0
30	36.3793072110557	38.0	35.0	40.0	31.0	41.0
31	36.26133037487001	38.0	35.0	40.0	31.0	41.0
32	36.12888285895619	38.0	35.0	40.0	30.0	41.0
33	35.98956724528197	38.0	35.0	40.0	30.0	41.0
34	35.87382564467253	38.0	35.0	40.0	30.0	41.0
35	35.71790939672973	38.0	35.0	40.0	29.0	41.0
36	35.58660855021061	38.0	35.0	40.0	29.0	41.0
37	35.53944165295199	38.0	34.0	40.0	29.0	41.0
38	35.447691216681285	38.0	34.0	40.0	28.0	41.0
39	35.39853689156749	38.0	34.0	40.0	28.0	41.0
40	35.311026996639946	38.0	34.0	40.0	27.0	41.0
41	35.243910832921976	37.0	34.0	40.0	27.0	41.0
42	35.208788185481346	37.0	34.0	40.0	27.0	41.0
43	35.159068719713076	37.0	34.0	40.0	27.0	41.0
44	35.038543287887016	37.0	34.0	40.0	27.0	41.0
45	34.956350581876364	37.0	34.0	40.0	27.0	41.0
46	34.94782679084532	37.0	34.0	40.0	27.0	41.0
47	34.9003425606533	37.0	34.0	40.0	27.0	41.0
48	34.78225051125862	36.0	34.0	40.0	26.0	41.0
49	34.74142083710342	36.0	34.0	40.0	26.0	41.0
50	34.64713273058285	36.0	34.0	40.0	26.0	41.0
51	34.23044975859853	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	4.0
11	6.0
12	1.0
13	2.0
14	12.0
15	35.0
16	123.0
17	249.0
18	650.0
19	1331.0
20	2312.0
21	3927.0
22	6225.0
23	9477.0
24	14156.0
25	21000.0
26	27742.0
27	32269.0
28	35545.0
29	40401.0
30	48047.0
31	59802.0
32	75768.0
33	100935.0
34	174263.0
35	266684.0
36	143805.0
37	179565.0
38	273199.0
39	495948.0
40	175.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.12630943288284	23.773500763287508	30.473546153318985	13.626643650510662
2	31.18692449263976	26.031133390079148	29.243595486423217	13.538346630857873
3	28.878339817387065	25.107540605207042	32.38782355295686	13.626296024449038
4	25.689168667171884	28.16272673909869	31.548554918461825	14.599549675267598
5	22.818075363343727	32.03543004820084	30.856878377559642	14.289616210895792
6	21.98670280653418	40.18115290679947	27.779295193126142	10.052849093540214
7	87.76217212654781	3.5913248426495468	6.874652994699199	1.7718500361034495
8	88.54626753897634	2.69723061214963	6.657486027915366	2.099015820958673
9	83.49978000236385	5.255261817051356	8.550856202989783	2.694101977595004
10	45.25480493708465	29.140747833048113	14.918869043303282	10.685578186563955
11	37.89521358641835	23.029283026213985	24.835448720686433	14.240054666681235
12	36.024637748813355	21.85217152068524	26.65715826620012	15.466032464301286
13	23.59124538526403	33.8378711777273	27.230343980954064	15.340539456054604
14	16.784131168252006	36.850497949502845	30.18809549585878	16.177275386386366
15	15.461414003768267	27.09039966071696	42.35664646131568	15.091539874199094
16	18.9416474892956	24.08303694073174	40.22008702570148	16.755228544271173
17	19.909239801396264	24.23648901650628	28.798783110140846	27.055488071956606
18	22.051708880058083	25.79762799839893	33.57084470153323	18.57981842000975
19	26.476740340216658	27.38588181309835	27.727846536005618	18.40953131067937
20	28.015631254165307	25.992397914640918	28.428809658839782	17.563161172353993
21	22.70852349306585	27.57593394707542	31.09296613426908	18.62257642558965
22	21.79382000319816	24.971867119441335	30.50359097721659	22.730721900143916
23	19.455389147511642	28.881766417137367	29.13017006860152	22.532674366749468
24	19.925876191488324	26.39385635495203	35.73089372674009	17.949373726819548
25	18.56213915173282	27.58303545090576	33.18920094673475	20.66562445062667
26	17.472182465940094	33.452254553653106	28.604311159094543	20.471251821312258
27	17.068091999733817	33.495260863562734	31.44575692595267	17.99089021075078
28	16.305052794466587	29.41005870907572	35.89959168836019	18.3852968080975
29	16.271482049086785	27.082702226495265	36.07136862366897	20.574447100748987
30	19.01206659720767	29.926929001846393	33.073640111677356	17.987364289268584
31	25.12308445624828	27.762509820436243	29.015701772594948	18.09870395072053
32	26.065101422386523	27.56634939994776	28.939770308562824	17.428778869102896
33	24.519456630669158	28.85326108008411	27.753769508029663	18.87351278121707
34	18.88632528463125	29.01654600731604	29.77084490017669	22.326283807876017
35	19.098327521356655	28.55902044935138	31.279144720702323	21.063507308589642
36	26.337193306907132	26.45335007235588	28.9996116520283	18.209844968708687
37	20.11563036026972	30.991558646006425	30.779159122353448	18.11365187137041
38	20.238441681755294	30.947410136180025	27.67197806181586	21.14217012024882
39	19.662921906301865	30.759294775974865	29.62633178027252	19.951451537450748
40	22.357371509958494	28.650893051352316	28.13799562785736	20.85373981083183
41	18.72949626997236	26.202364055862514	30.324315251149898	24.74382442301523
42	21.90188204749764	26.24090088783696	28.248938002381735	23.608279062283664
43	21.314195359887332	26.641564754292936	29.480726121317524	22.563513764502215
44	19.258881100961535	30.262189507850884	29.54384508193546	20.935084309252115
45	18.19445010026529	34.026433485725974	26.554856882350432	21.224259531658305
46	21.582513018596007	31.38919319963966	27.550954531504356	19.477339250259977
47	19.80852756525686	29.450830280017758	29.536991882434855	21.203650272290528
48	21.371901286117108	27.094769816920248	31.66342050139597	19.869908395566675
49	21.209013645812743	25.994384349278775	31.03401868639064	21.76258331851784
50	18.85613147813581	30.663449304698215	30.121251970294853	20.359167246871117
51	18.2103912382341	32.560643366450506	26.926270498763945	22.30269489655145
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1172.0
1	2029.5
2	2887.0
3	10489.5
4	18092.0
5	13092.5
6	8093.0
7	8177.0
8	8261.0
9	8906.5
10	9552.0
11	10004.5
12	10457.0
13	10367.5
14	10278.0
15	9806.5
16	9335.0
17	9395.5
18	9456.0
19	9198.0
20	8940.0
21	10130.0
22	11320.0
23	12844.5
24	14369.0
25	15126.0
26	20943.0
27	26003.0
28	28963.0
29	31923.0
30	39810.5
31	47698.0
32	51445.0
33	55192.0
34	62132.0
35	69072.0
36	76558.0
37	84044.0
38	93623.5
39	103203.0
40	123661.5
41	144120.0
42	165366.5
43	186613.0
44	189338.0
45	192063.0
46	192336.5
47	192610.0
48	179765.5
49	166921.0
50	157147.0
51	147373.0
52	129516.0
53	111659.0
54	99920.0
55	88181.0
56	77133.0
57	66085.0
58	58522.0
59	50959.0
60	43323.5
61	35688.0
62	29984.5
63	24281.0
64	21073.5
65	17866.0
66	14933.0
67	12000.0
68	10351.0
69	8702.0
70	6987.5
71	5273.0
72	4608.5
73	3944.0
74	2888.0
75	1422.0
76	1012.0
77	872.0
78	732.0
79	491.5
80	251.0
81	173.5
82	96.0
83	105.0
84	114.0
85	77.0
86	40.0
87	23.0
88	6.0
89	5.5
90	5.0
91	3.0
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2013658.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.147026182440698
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.85884805975603	15.686242503945765
2	8.309763876935614	3.348340607970434
3	2.986160736115539	1.8048677560648843
4	1.5472079662312277	1.2468655762136547
5	0.9285223554893761	0.9353482103512984
6	0.68274518634982	0.8253171087195106
7	0.5191084591220149	0.7320944202220374
8	0.38455625085357786	0.6198131883654613
9	0.3345632975004557	0.6066409962982839
>10	3.7630978725121764	18.476530494713963
>50	1.5452605547880027	22.588047902502105
>100	1.135914767793639	31.929861025754676
>500	0.0035005077492348546	0.45780683982378545
>1k	5.000725356049793E-4	0.19490038108255126
>5k	0.0	0.0
>10k+	2.5003626780248963E-4	0.5473229879715481
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10865	0.5395653085081975	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2842	0.14113618101981568	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.966086594645168E-5	0.0	0.0	0.03818920591282134	0.0
2	4.966086594645168E-5	0.0	0.0	0.13795788559924277	0.0
3	4.966086594645168E-5	0.0	0.0	0.22769507036448097	0.0
4	4.966086594645168E-5	0.0	0.0	0.4380088376477038	0.0
5	4.966086594645168E-5	0.0	0.0	0.7689985091808043	0.0
6	4.966086594645168E-5	0.0	0.0	1.2235444151886765	0.0
7	4.966086594645168E-5	0.0	0.0	1.4405623993746703	0.0
8	4.966086594645168E-5	0.0	0.0	2.0164794617556705	0.0
9	4.966086594645168E-5	0.0	0.0	2.242585384409865	0.0
10	4.966086594645168E-5	0.0	0.0	2.642454677010694	0.0
11	4.966086594645168E-5	0.0	0.0	3.131316241387564	0.0
12	4.966086594645168E-5	0.0	0.0	3.4980120755361637	0.0
13	4.966086594645168E-5	0.0	0.0	3.666958341485992	0.0
14	4.966086594645168E-5	0.0	0.0	3.735639319089935	0.0
15	4.966086594645168E-5	0.0	0.0	3.836599859559071	0.0
16	4.966086594645168E-5	0.0	0.0	4.050787174386117	0.0
17	4.966086594645168E-5	0.0	0.0	4.302617425600574	0.0
18	4.966086594645168E-5	0.0	0.0	4.614686307208076	0.0
19	4.966086594645168E-5	0.0	0.0	4.79406135500666	0.0
20	4.966086594645168E-5	0.0	0.0	4.987242123538357	0.0
21	4.966086594645168E-5	0.0	0.0	5.240065592071742	0.0
22	4.966086594645168E-5	0.0	0.0	5.521295075926498	0.0
23	4.966086594645168E-5	0.0	0.0	5.815784010988956	0.0
24	4.966086594645168E-5	0.0	0.0	6.025899134808394	0.0
25	4.966086594645168E-5	0.0	0.0	6.210389251799461	0.0
26	4.966086594645168E-5	0.0	0.0	6.393588186275922	0.0
27	4.966086594645168E-5	0.0	0.0	6.601567892859661	0.0
28	4.966086594645168E-5	0.0	0.0	6.800757626170879	0.0
29	4.966086594645168E-5	0.0	0.0	7.032028278883504	0.0
30	4.966086594645168E-5	0.0	0.0	7.308887606534973	0.0
31	4.966086594645168E-5	0.0	0.0	7.554609571238015	0.0
32	4.966086594645168E-5	0.0	0.0	7.772471790145099	0.0
33	4.966086594645168E-5	0.0	0.0	7.995498739110614	0.0
34	4.966086594645168E-5	0.0	0.0	8.23471513037467	0.0
35	4.966086594645168E-5	0.0	0.0	8.508296840873674	0.0
36	4.966086594645168E-5	0.0	0.0	8.74597374529339	0.0
37	4.966086594645168E-5	0.0	0.0	8.998300605167312	0.0
38	4.966086594645168E-5	0.0	0.0	9.291200392519485	0.0
39	4.966086594645168E-5	0.0	0.0	9.763723531999972	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGGAT	25	3.8918537E-5	45.000004	10
CACCGCA	25	3.8918537E-5	45.000004	28
GCGCTAA	25	3.8918537E-5	45.000004	36
TCGACTC	25	3.8918537E-5	45.000004	14
CGAGGTA	20	7.034551E-4	45.0	13
GCGATAC	35	1.2125383E-7	45.0	9
TACTCGC	40	6.8193913E-9	45.0	45
CTACGCG	60	0.0	44.999996	1
GCGATCG	30	2.1663182E-6	44.999996	9
CGTTTTT	7145	0.0	41.410076	1
CGTAAGG	280	0.0	40.98214	2
CGCATCG	55	6.184564E-11	40.909092	21
CCGCTCG	50	1.0822987E-9	40.500004	19
GGCGATA	635	0.0	40.3937	8
TCGTAAG	90	0.0	40.000004	1
TTAGTCG	45	1.929584E-8	40.000004	15
TGTAGCG	125	0.0	39.6	1
GTTAGCG	115	0.0	39.130432	1
TACGCGG	115	0.0	39.130432	2
CGATGCG	75	0.0	39.0	10
>>END_MODULE