FastQCFastQC Report
Thu 26 May 2016
SRR1547629_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547629_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1730960
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65330.3774206220825438No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC29890.17267874474280168No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACTT207.034278E-445.036
CAACGTT207.034278E-445.016
CCCGTTA253.8916267E-545.039
ACTCGCG207.034278E-445.01
CGTAATC253.8916267E-545.022
CGACTCG207.034278E-445.015
TATCGCC207.034278E-445.037
TATCGAT207.034278E-445.013
TCGCGGA253.8916267E-545.03
ATCGCCT1650.043.63636814
TTTACGG2200.042.9545442
ATTTACG750.042.01
TAGGACG700.041.7857171
CGTAAGG2700.041.6666642
ATTAGCG1100.040.9090921
GGGCGAT23850.040.4716957
TTTAGCG900.040.01
TCACGAC1550.039.19354625
TAGGGCG6900.039.1304325
CGGGATC4950.039.0909086
Produced by FastQC (version 0.11.5)