##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547629_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1730960
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.32198779867819	31.0	31.0	33.0	30.0	34.0
2	31.731792762397745	31.0	31.0	34.0	30.0	34.0
3	31.88543813837408	31.0	31.0	34.0	30.0	34.0
4	35.64358795119471	37.0	35.0	37.0	33.0	37.0
5	35.53871897675278	37.0	35.0	37.0	33.0	37.0
6	35.58536130239867	37.0	35.0	37.0	33.0	37.0
7	35.90232992096871	37.0	35.0	37.0	35.0	37.0
8	35.91526378425845	37.0	35.0	37.0	35.0	37.0
9	37.73452708323705	39.0	37.0	39.0	35.0	39.0
10	37.150381869020656	39.0	37.0	39.0	33.0	39.0
11	36.861520774599065	39.0	37.0	39.0	32.0	39.0
12	36.19045731848223	38.0	35.0	39.0	32.0	39.0
13	35.95531150344318	38.0	35.0	39.0	31.0	39.0
14	36.92523917363775	39.0	35.0	41.0	31.0	41.0
15	37.16306673753293	39.0	35.0	41.0	32.0	41.0
16	37.26241045431437	39.0	35.0	41.0	32.0	41.0
17	37.19890061006609	39.0	35.0	41.0	32.0	41.0
18	37.11460807875399	38.0	35.0	41.0	32.0	41.0
19	37.08102671350002	38.0	35.0	41.0	32.0	41.0
20	36.93577205712437	38.0	35.0	41.0	32.0	41.0
21	36.74679310902621	38.0	35.0	41.0	31.0	41.0
22	36.69497850903545	38.0	35.0	41.0	31.0	41.0
23	36.62917282895041	38.0	35.0	40.0	31.0	41.0
24	36.55069614549152	38.0	35.0	40.0	31.0	41.0
25	36.45062624208531	38.0	35.0	40.0	31.0	41.0
26	36.3215158062578	38.0	35.0	40.0	31.0	41.0
27	36.25907242223968	38.0	35.0	40.0	30.0	41.0
28	36.298978601469706	38.0	35.0	40.0	31.0	41.0
29	36.335418496094654	38.0	35.0	40.0	31.0	41.0
30	36.36951576004067	38.0	35.0	40.0	31.0	41.0
31	36.27114260294865	38.0	35.0	40.0	30.0	41.0
32	36.165185792854835	38.0	35.0	40.0	30.0	41.0
33	36.05074813976059	38.0	35.0	40.0	30.0	41.0
34	35.96415110690022	38.0	35.0	40.0	30.0	41.0
35	35.82087512131996	38.0	35.0	40.0	29.0	41.0
36	35.713373503720476	38.0	35.0	40.0	29.0	41.0
37	35.683077020843925	38.0	35.0	40.0	29.0	41.0
38	35.61424874058326	38.0	34.0	40.0	29.0	41.0
39	35.562487868003885	38.0	34.0	40.0	29.0	41.0
40	35.46889009566946	38.0	34.0	40.0	28.0	41.0
41	35.41020474187734	38.0	34.0	40.0	28.0	41.0
42	35.36070157600407	38.0	34.0	40.0	27.0	41.0
43	35.32816471784443	38.0	34.0	40.0	27.0	41.0
44	35.19945983731571	37.0	34.0	40.0	27.0	41.0
45	35.10709895087119	37.0	34.0	40.0	27.0	41.0
46	35.108153856819335	37.0	34.0	40.0	27.0	41.0
47	35.04854473817997	37.0	34.0	40.0	27.0	41.0
48	34.928819267920694	37.0	34.0	40.0	27.0	41.0
49	34.89169767065675	37.0	34.0	40.0	26.0	41.0
50	34.788328442020614	36.0	34.0	40.0	26.0	41.0
51	34.34502299302121	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	4.0
11	6.0
12	5.0
13	9.0
14	17.0
15	35.0
16	98.0
17	245.0
18	615.0
19	1192.0
20	2181.0
21	3424.0
22	5381.0
23	8139.0
24	11986.0
25	17060.0
26	22663.0
27	27171.0
28	30978.0
29	35477.0
30	43465.0
31	51985.0
32	64961.0
33	85383.0
34	143968.0
35	221382.0
36	121796.0
37	155624.0
38	238630.0
39	436943.0
40	136.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.92368396727827	23.95924804732634	28.330695105606136	12.786372879789251
2	31.200432130147433	25.529070573554556	29.28323011508065	13.987267181217359
3	29.07224892545177	26.242605259509173	30.991241854231177	13.693903960807877
4	26.532155566853078	28.056627536164903	30.169963488468827	15.241253408513195
5	23.562358460045292	31.887334196053057	29.41477561584323	15.13553172805842
6	22.268567731201184	41.13902112122753	25.99823219485141	10.594178952719878
7	87.90653741276518	3.955550677080926	6.318170263899801	1.8197416462541016
8	88.13658316772197	3.2915838609788786	6.161205342700004	2.410627628599159
9	82.79671396219439	5.94242501270971	8.215152285436982	3.0457087396589175
10	44.47098719785553	30.917814392013682	13.876923787955816	10.734274622174977
11	37.67054120256967	22.70965244719693	24.636155659287333	14.983650690946066
12	35.16337754771918	22.104150298100475	26.85376900679392	15.878703147386423
13	23.52394047233905	33.910951148495634	26.30158986920553	16.26351850995979
14	17.558118038545086	36.754922124139206	28.752253084993303	16.934706752322413
15	16.684209918195684	26.75266903914591	41.13387946573	15.42924157692841
16	19.473240282848824	23.919039145907472	39.680004159541525	16.927716411702175
17	20.508850580024955	23.260733927993716	28.655832601562135	27.57458289041919
18	22.349678790959928	25.53022600175625	32.99082590007857	19.129269307205252
19	27.344248278411982	26.969138512732822	27.443268475296946	18.243344733558256
20	29.013495401395755	25.789619633036004	27.047303230577253	18.14958173499099
21	24.003789804501547	27.429345565466562	29.92137311087489	18.645491519157
22	23.538672181910616	23.948964736331284	28.752773027684057	23.75959005407404
23	20.275569626103433	29.43597772334427	28.265124555160142	22.023328095392152
24	20.850337385034894	25.690137264870362	35.4700859638582	17.98943938623654
25	19.4156421869945	26.997504275084346	33.30614225632019	20.28071128160096
26	18.265644497850904	33.510999676480104	28.271652724499702	19.951703101169294
27	18.114861117530157	33.14305356565143	30.659171789065027	18.082913527753387
28	16.600325830752876	29.128864907334655	36.49679946388132	17.77400979803115
29	17.84605074640662	27.15522022461524	35.727977538475756	19.27075149050238
30	19.85522484632805	29.85331838979526	31.964747885566393	18.326708878310303
31	26.26675370892453	27.732876554050932	27.718780329990295	18.281589407034247
32	26.43556176919166	28.426075703655773	27.991808014050008	17.146554513102554
33	25.85923418218792	28.045188796968155	26.6500670148357	19.445510006008227
34	19.667987706243935	28.767273651615287	29.325980958543234	22.23875768359754
35	19.865392614502937	28.27812312242917	31.196157045801176	20.66032721726672
36	26.853595692563665	25.955019180108145	28.969184729860885	18.2222003974673
37	19.91403614179415	31.25231085640338	29.43395572399131	19.399697277811157
38	19.72321717428479	32.24395711050516	26.981212737440497	21.05161297776956
39	19.854647132227203	30.763911355548366	29.278781716504138	20.102659795720292
40	23.28262929241577	27.55338078291815	27.995967555576094	21.168022369089982
41	18.67553265240098	25.88384480288395	29.242790128021447	26.19783241669363
42	21.685249803577207	26.371493275407865	27.5936474557471	24.349609465267825
43	21.23677034709063	26.738630586495354	28.424227018533067	23.600372047880942
44	19.54724545916717	30.455585339926976	29.718537690067937	20.278631510837915
45	19.174735406941814	33.54670240791237	26.307482553034156	20.97107963211166
46	21.76636086333595	31.42672274344872	27.138582058510885	19.66833433470444
47	20.429645976799	28.81793917825946	28.695752645930583	22.056662199010955
48	21.47854369829459	26.770058233581366	31.279752276193555	20.47164579193049
49	20.988006655266442	25.757209871978553	31.985834450247264	21.26894902250774
50	19.490918334334705	31.16062762859916	29.674746961223832	19.673707075842305
51	18.275407866155195	32.0958312150483	27.57851134630494	22.050249572491566
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	581.0
1	1626.0
2	2671.0
3	7277.5
4	11884.0
5	8892.0
6	5900.0
7	6170.5
8	6441.0
9	6725.0
10	7009.0
11	7039.5
12	7070.0
13	6894.0
14	6718.0
15	6793.0
16	6868.0
17	6615.5
18	6363.0
19	6434.5
20	6506.0
21	7367.5
22	8229.0
23	8866.0
24	9503.0
25	11937.5
26	17214.0
27	20056.0
28	23991.0
29	27926.0
30	31183.0
31	34440.0
32	39868.0
33	45296.0
34	52486.0
35	59676.0
36	63693.5
37	67711.0
38	78381.0
39	89051.0
40	104872.5
41	120694.0
42	140787.5
43	160881.0
44	165866.5
45	170852.0
46	166159.0
47	161466.0
48	156723.0
49	151980.0
50	140243.5
51	128507.0
52	113344.0
53	98181.0
54	87273.0
55	76365.0
56	68644.0
57	60923.0
58	53632.5
59	46342.0
60	41172.0
61	36002.0
62	30817.0
63	25632.0
64	21760.0
65	17888.0
66	14783.0
67	11678.0
68	10036.5
69	8395.0
70	6558.0
71	4721.0
72	3836.0
73	2951.0
74	2205.0
75	1224.0
76	989.0
77	710.5
78	432.0
79	305.5
80	179.0
81	136.5
82	94.0
83	59.0
84	24.0
85	20.0
86	16.0
87	9.0
88	2.0
89	1.0
90	0.0
91	2.5
92	5.0
93	10.0
94	15.0
95	10.0
96	5.0
97	4.0
98	3.0
99	5.5
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1730960.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.07119966026411
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.3733850826535	14.163010898793965
2	7.798794071947632	2.8186712956689965
3	2.5583668045689003	1.386982719886695
4	1.2603277267726969	0.9110253595150479
5	0.7600555958909229	0.6867558213122942
6	0.5423285019298725	0.5880315983895995
7	0.4144104436576144	0.5242225708044768
8	0.3427958799833549	0.4955786231916112
9	0.2844360181434221	0.46260900669962585
>10	4.453907266310901	20.628414129391516
>50	1.986970237630082	25.750082610577625
>100	1.2193579107607935	30.453667353670216
>500	0.003567270483606733	0.426991772004797
>1k	9.728919500745635E-4	0.3210935930296847
>5k	3.2429731669152124E-4	0.3828626470638675
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6533	0.3774206220825438	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2989	0.17267874474280168	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03864907334658224	0.0
2	0.0	0.0	0.0	0.1218976752784582	0.0
3	0.0	0.0	0.0	0.1861394832925082	0.0
4	0.0	0.0	0.0	0.3615912557193696	0.0
5	0.0	0.0	0.0	0.6127235753570273	0.0
6	0.0	0.0	0.0	0.978127744141979	0.0
7	0.0	0.0	0.0	1.1345149512409298	0.0
8	0.0	0.0	0.0	1.5863451495124092	0.0
9	0.0	0.0	0.0	1.7457942413458427	0.0
10	0.0	0.0	0.0	2.0381753477838886	0.0
11	0.0	0.0	0.0	2.3808753524056017	0.0
12	0.0	0.0	0.0	2.6682303461662893	0.0
13	0.0	0.0	0.0	2.7965406479641355	0.0
14	0.0	0.0	0.0	2.849517031011693	0.0
15	0.0	0.0	0.0	2.9288371770578174	0.0
16	0.0	0.0	0.0	3.1040578638443406	0.0
17	0.0	0.0	0.0	3.292219346489809	0.0
18	0.0	0.0	0.0	3.5296020705273374	0.0
19	0.0	0.0	0.0	3.661840828210935	0.0
20	0.0	0.0	0.0	3.803842954198826	0.0
21	0.0	0.0	0.0	3.990040208901419	0.0
22	0.0	0.0	0.0	4.183343347044414	0.0
23	0.0	0.0	0.0	4.392475851550585	0.0
24	0.0	0.0	0.0	4.545858945325137	0.0
25	0.0	0.0	0.0	4.681448444793641	0.0
26	0.0	0.0	0.0	4.820446457457134	0.0
27	5.7771410084577346E-5	0.0	0.0	4.966896982021537	0.0
28	5.7771410084577346E-5	0.0	0.0	5.116178305680085	0.0
29	5.7771410084577346E-5	0.0	0.0	5.281635624162314	0.0
30	5.7771410084577346E-5	0.0	0.0	5.476729676017932	0.0
31	5.7771410084577346E-5	0.0	0.0	5.6514304201136945	0.0
32	5.7771410084577346E-5	0.0	0.0	5.827286592411148	0.0
33	5.7771410084577346E-5	0.0	0.0	5.999156537412765	0.0
34	5.7771410084577346E-5	0.0	0.0	6.183909506863244	0.0
35	5.7771410084577346E-5	0.0	0.0	6.406156121458612	0.0
36	1.7331423025373203E-4	0.0	0.0	6.595935203586449	0.0
37	1.7331423025373203E-4	0.0	0.0	6.792184683643758	0.0
38	1.7331423025373203E-4	0.0	0.0	7.008596385820585	0.0
39	1.7331423025373203E-4	0.0	0.0	7.336506909460646	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACTT	20	7.034278E-4	45.0	36
CAACGTT	20	7.034278E-4	45.0	16
CCCGTTA	25	3.8916267E-5	45.0	39
ACTCGCG	20	7.034278E-4	45.0	1
CGTAATC	25	3.8916267E-5	45.0	22
CGACTCG	20	7.034278E-4	45.0	15
TATCGCC	20	7.034278E-4	45.0	37
TATCGAT	20	7.034278E-4	45.0	13
TCGCGGA	25	3.8916267E-5	45.0	3
ATCGCCT	165	0.0	43.636368	14
TTTACGG	220	0.0	42.954544	2
ATTTACG	75	0.0	42.0	1
TAGGACG	70	0.0	41.785717	1
CGTAAGG	270	0.0	41.666664	2
ATTAGCG	110	0.0	40.909092	1
GGGCGAT	2385	0.0	40.471695	7
TTTAGCG	90	0.0	40.0	1
TCACGAC	155	0.0	39.193546	25
TAGGGCG	690	0.0	39.130432	5
CGGGATC	495	0.0	39.090908	6
>>END_MODULE