FastQCFastQC Report
Thu 26 May 2016
SRR1547628_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547628_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1390201
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96920.6971653739279428No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC37730.27139960336670743No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT19890.14307283623015665No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGAT351.2121745E-745.0000044
TAGACGC253.891228E-545.00000436
GCGCGCG253.891228E-545.0000041
TTCGTAA253.891228E-545.00000420
CGCGAGT253.891228E-545.00000427
CCGCTAG253.891228E-545.0000041
ACCAACG253.891228E-545.00000433
TCGAGCG253.891228E-545.0000041
ACCCGCG253.891228E-545.00000424
ACTCCGA253.891228E-545.00000421
CGTCAGG253.891228E-545.0000042
CCCGCGA253.891228E-545.00000425
ATTTCGC351.2121745E-745.00000418
TCGTTAC406.8175723E-945.043
GTCGATA207.033798E-445.030
GGTCGAA207.033798E-445.09
CGCATCG453.8562575E-1045.021
TAGCGCG453.8562575E-1045.01
CCTACGG453.8562575E-1045.02
GCGTTGC207.033798E-445.035