##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547628_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1390201
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.329562415794552	31.0	31.0	33.0	30.0	34.0
2	31.7310741396388	31.0	31.0	34.0	30.0	34.0
3	31.823484517706433	31.0	31.0	34.0	30.0	34.0
4	35.60342497236011	37.0	35.0	37.0	33.0	37.0
5	35.51115989702208	37.0	35.0	37.0	33.0	37.0
6	35.57043909477838	37.0	35.0	37.0	33.0	37.0
7	35.92937208360517	37.0	35.0	37.0	35.0	37.0
8	35.981468147411775	37.0	35.0	37.0	35.0	37.0
9	37.8571113098034	39.0	38.0	39.0	35.0	39.0
10	37.20633491128261	39.0	37.0	39.0	33.0	39.0
11	36.81081368809259	39.0	35.0	39.0	32.0	39.0
12	35.949702237302375	37.0	35.0	39.0	32.0	39.0
13	35.61072895214433	37.0	35.0	39.0	31.0	39.0
14	36.56117424746493	38.0	35.0	41.0	31.0	41.0
15	36.89414264555989	38.0	35.0	41.0	32.0	41.0
16	37.04860520169385	38.0	35.0	41.0	32.0	41.0
17	36.97404260247259	38.0	35.0	41.0	32.0	41.0
18	36.90763781640209	38.0	35.0	40.0	32.0	41.0
19	36.84292271405358	38.0	35.0	40.0	32.0	41.0
20	36.647332292236875	38.0	35.0	40.0	32.0	41.0
21	36.448311431224695	38.0	35.0	40.0	31.0	41.0
22	36.35853736258282	38.0	35.0	40.0	31.0	41.0
23	36.369645108872746	37.0	35.0	40.0	31.0	41.0
24	36.27441859126846	37.0	35.0	40.0	31.0	41.0
25	36.178044038236195	37.0	35.0	40.0	31.0	41.0
26	36.06772545840494	37.0	35.0	40.0	31.0	41.0
27	36.02996113511643	37.0	35.0	40.0	31.0	41.0
28	36.07335270223514	37.0	35.0	40.0	31.0	41.0
29	36.124711462587065	37.0	35.0	40.0	31.0	41.0
30	36.129170530016886	37.0	35.0	40.0	31.0	41.0
31	35.97968279407078	37.0	35.0	40.0	30.0	41.0
32	35.795986335788854	37.0	35.0	40.0	30.0	41.0
33	35.653315599686664	37.0	35.0	40.0	30.0	41.0
34	35.56416590118983	37.0	35.0	40.0	30.0	41.0
35	35.384355211944175	37.0	34.0	40.0	29.0	41.0
36	35.224563929964084	37.0	34.0	40.0	28.0	41.0
37	35.18582348883363	37.0	34.0	40.0	27.0	41.0
38	35.14450931915601	37.0	34.0	40.0	28.0	41.0
39	35.1312896480437	36.0	34.0	40.0	27.0	41.0
40	35.032167290916924	36.0	34.0	40.0	27.0	41.0
41	34.995028057093904	36.0	34.0	40.0	27.0	41.0
42	34.952997444254464	36.0	34.0	40.0	27.0	41.0
43	34.89691994179259	36.0	34.0	40.0	27.0	41.0
44	34.79223148307331	36.0	34.0	40.0	27.0	41.0
45	34.694758527723685	36.0	34.0	40.0	26.0	41.0
46	34.673946429329284	36.0	34.0	40.0	26.0	41.0
47	34.6281048567797	35.0	34.0	40.0	26.0	41.0
48	34.54041825606513	35.0	34.0	40.0	26.0	41.0
49	34.53192452026721	36.0	34.0	40.0	26.0	41.0
50	34.405800312328935	35.0	34.0	40.0	25.0	41.0
51	33.99782909090124	35.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	2.0
12	3.0
13	1.0
14	4.0
15	19.0
16	65.0
17	164.0
18	416.0
19	843.0
20	1615.0
21	2745.0
22	4436.0
23	6883.0
24	10615.0
25	15581.0
26	20904.0
27	23954.0
28	25803.0
29	29226.0
30	34352.0
31	42718.0
32	54333.0
33	71447.0
34	129912.0
35	228769.0
36	94883.0
37	110182.0
38	164422.0
39	315790.0
40	111.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.6344909836779	24.959700072147843	30.567018726069108	13.838790218105151
2	30.413299947273813	26.18671688482457	30.344532912866555	13.055450255035064
3	27.454231438475446	25.44373079863991	34.26209591274931	12.83994185013534
4	24.27828781593453	27.525875754657058	33.749148504424895	14.44668792498351
5	22.662118643275324	31.942071686036765	32.337410201834125	13.058399468853784
6	19.94884193005184	41.294028705201626	28.218581341834742	10.538548022911796
7	88.98044239645921	3.2253609370155827	6.585019000849518	1.2091776656756827
8	90.10243842437173	2.346998743347185	5.936983213218808	1.6135796190622795
9	86.54331280153013	4.25945600672133	7.356562108644721	1.8406690831038102
10	50.530462861125834	27.75087918941218	13.062355731293533	8.656302218168452
11	40.79043246264389	21.111911155293374	25.17952439970911	12.918131982353632
12	40.679441318197874	21.67593031511271	24.559973701644584	13.08465466504484
13	20.755487875494264	41.56514058039089	24.00861458163244	13.670756962482402
14	13.753550745539675	43.38538096289673	26.73296882968722	16.128099461876378
15	12.181116255850773	26.82691207962014	46.565640508099186	14.426331156429898
16	14.120116443593409	22.20592561795021	45.79733434230015	17.87662359615624
17	14.550198136816187	24.109175579646397	28.27806914251968	33.06255714101774
18	19.169026637155344	26.772027929774183	34.913296710331814	19.145648722738652
19	25.465166547858907	29.376111799660627	25.20196719754913	19.95675445493134
20	28.331514651478457	25.433444516296568	28.580903049271296	17.65413778295369
21	19.01976764511031	31.22231965018008	30.40495583012816	19.352956874581444
22	20.444813375907515	25.859066422769082	27.97178249763883	25.724337703684576
23	16.585515331955595	32.398840167716756	26.624063714527612	24.391580785800038
24	17.699814631121686	25.468475421899424	40.52442776260411	16.307282184374778
25	14.298651777692578	29.09866990456776	35.872222793682354	20.730455524057312
26	14.583358809265711	38.694980078420315	27.191895272697973	19.529765839615997
27	14.677230127154276	38.069674816807066	30.357696476984263	16.895398579054397
28	13.024375611871953	31.044215908347066	39.317911582569714	16.613496897211267
29	12.718160899035464	25.966173236819714	39.792087618984596	21.523578245160234
30	15.780236095355995	34.90732635065002	32.940704257873506	16.37173329612049
31	26.63262362780634	30.34956815597169	26.07328005087034	16.94452816535163
32	26.135645133329643	29.401288015186296	27.40538958035565	17.05767727112842
33	24.776992679475846	31.904307362748263	24.53048156345737	18.78821839431852
34	16.54508952302581	30.61168852561608	27.57536500117609	25.267856950182022
35	17.04480143518815	28.703187524681685	32.33654701730181	21.915464022828353
36	27.076012749235538	27.559971543683254	28.525227646937385	16.838788060143823
37	16.840802157385877	33.8282018211755	31.804177957000462	17.526818064438164
38	16.5624970777607	34.51982842768779	27.547599232053493	21.370075262498013
39	17.341664982257964	34.86093018203842	29.96286148549742	17.834543350206193
40	23.315333538099885	29.26943657787615	26.71376297384335	20.701466910180613
41	15.709023371440534	25.85698039348267	31.518895469072454	26.91510076600434
42	19.800158394361677	26.24433445235617	27.40049820133923	26.55500895194292
43	20.364393350314092	27.854317469200495	27.561194388437354	24.220094792048055
44	16.997757878177328	32.84150996870237	28.626507965394932	21.534224187725375
45	15.029769076557994	40.73353421555588	23.533287632507818	20.70340907537831
46	21.1888065107132	36.36912935611469	24.75152873577274	17.69053539739937
47	18.39978535477963	30.720737504864402	28.181392474901113	22.69808466545485
48	20.28555582969657	26.91826577595614	33.61377239694116	19.18240599740613
49	19.581484979510158	26.142262881410673	32.48630953365736	21.789942605421807
50	17.339938613193347	33.850644619015526	29.91445121964378	18.894965548147354
51	15.74204017980134	35.42293524461571	25.92301401020428	22.912010565378676
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	764.0
1	1088.0
2	1412.0
3	7491.5
4	13571.0
5	9683.5
6	5796.0
7	6169.0
8	6542.0
9	7599.0
10	8656.0
11	9376.0
12	10096.0
13	10027.5
14	9959.0
15	9204.0
16	8449.0
17	8012.0
18	7575.0
19	7332.0
20	7089.0
21	7521.5
22	7954.0
23	8459.5
24	8965.0
25	9992.0
26	13453.5
27	15888.0
28	17899.0
29	19910.0
30	24343.0
31	28776.0
32	35443.0
33	42110.0
34	47720.0
35	53330.0
36	54966.5
37	56603.0
38	64535.5
39	72468.0
40	95015.0
41	117562.0
42	134331.0
43	151100.0
44	153782.0
45	156464.0
46	152771.5
47	149079.0
48	133914.0
49	118749.0
50	108482.5
51	98216.0
52	84196.5
53	70177.0
54	59164.0
55	48151.0
56	39690.0
57	31229.0
58	26476.0
59	21723.0
60	17517.5
61	13312.0
62	10325.5
63	7339.0
64	6109.5
65	4880.0
66	3624.5
67	2369.0
68	1818.5
69	1268.0
70	960.0
71	652.0
72	523.0
73	394.0
74	318.5
75	191.0
76	139.0
77	115.5
78	92.0
79	93.0
80	94.0
81	51.5
82	9.0
83	15.5
84	22.0
85	11.5
86	1.0
87	1.5
88	2.0
89	2.0
90	2.0
91	1.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1390201.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.085248747046524
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.58960796535933	16.359961841354238
2	9.042529277079648	3.8132795821935037
3	3.266808428164797	2.0664440495040846
4	1.627489505477352	1.3726408422479082
5	1.000638869942267	1.0549359739348119
6	0.6461422237989124	0.8174441708861924
7	0.4921652176810924	0.7264198227615093
8	0.37385456904212383	0.6306253266778448
9	0.27779650199785005	0.5271667511115667
>10	3.2100241826004314	15.910579582645623
>50	1.3375950837259178	20.642370455809083
>100	1.1315452427532147	34.5963713724533
>500	0.0027657690015354766	0.3552229838683761
>1k	6.914422503838691E-4	0.42002766941300707
>5k	3.4572112519193457E-4	0.7065095751389906
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9692	0.6971653739279428	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3773	0.27139960336670743	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1989	0.14307283623015665	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.055243810067752794	0.0
2	0.0	0.0	0.0	0.19076378164020885	0.0
3	0.0	0.0	0.0	0.29894957635622477	0.0
4	0.0	0.0	0.0	0.5334480409667379	0.0
5	0.0	0.0	0.0	0.9251180225017821	0.0
6	0.0	0.0	0.0	1.4182121865830912	0.0
7	0.0	0.0	0.0	1.6641478462466939	0.0
8	0.0	0.0	0.0	2.3039833808204713	0.0
9	0.0	0.0	0.0	2.534885243213032	0.0
10	0.0	0.0	0.0	2.9543929259150294	0.0
11	0.0	0.0	0.0	3.509924104499997	0.0
12	0.0	0.0	0.0	3.931086224222253	0.0
13	0.0	0.0	0.0	4.113074296450657	0.0
14	0.0	0.0	0.0	4.1882432828058676	0.0
15	0.0	0.0	0.0	4.280316299585456	0.0
16	0.0	0.0	0.0	4.518339434369563	0.0
17	0.0	0.0	0.0	4.791896999067041	0.0
18	0.0	0.0	0.0	5.132854889328953	0.0
19	0.0	0.0	0.0	5.319662408529414	0.0
20	0.0	0.0	0.0	5.50459969457654	0.0
21	0.0	0.0	0.0	5.780890676959663	0.0
22	0.0	0.0	0.0	6.068331126218439	0.0
23	0.0	0.0	0.0	6.427343959614473	0.0
24	0.0	0.0	0.0	6.662345948535499	0.0
25	0.0	0.0	0.0	6.866561022470852	0.0
26	0.0	0.0	0.0	7.061640726772604	0.0
27	0.0	0.0	0.0	7.246578012819729	0.0
28	0.0	0.0	0.0	7.450505358577645	0.0
29	0.0	0.0	0.0	7.67493333697789	0.0
30	0.0	0.0	0.0	7.958201727663841	0.0
31	7.193204435905312E-5	0.0	0.0	8.22111334979618	0.0
32	7.193204435905312E-5	0.0	0.0	8.449641454724892	0.0
33	7.193204435905312E-5	0.0	0.0	8.682989006625661	0.0
34	7.193204435905312E-5	0.0	0.0	8.919501568478227	0.0
35	7.193204435905312E-5	0.0	0.0	9.22924095148831	0.0
36	7.193204435905312E-5	0.0	0.0	9.487045398471157	0.0
37	7.193204435905312E-5	0.0	0.0	9.742404155945795	0.0
38	7.193204435905312E-5	0.0	0.0	10.005891234433006	0.0
39	7.193204435905312E-5	0.0	0.0	10.253984855427381	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGGAT	35	1.2121745E-7	45.000004	4
TAGACGC	25	3.891228E-5	45.000004	36
GCGCGCG	25	3.891228E-5	45.000004	1
TTCGTAA	25	3.891228E-5	45.000004	20
CGCGAGT	25	3.891228E-5	45.000004	27
CCGCTAG	25	3.891228E-5	45.000004	1
ACCAACG	25	3.891228E-5	45.000004	33
TCGAGCG	25	3.891228E-5	45.000004	1
ACCCGCG	25	3.891228E-5	45.000004	24
ACTCCGA	25	3.891228E-5	45.000004	21
CGTCAGG	25	3.891228E-5	45.000004	2
CCCGCGA	25	3.891228E-5	45.000004	25
ATTTCGC	35	1.2121745E-7	45.000004	18
TCGTTAC	40	6.8175723E-9	45.0	43
GTCGATA	20	7.033798E-4	45.0	30
GGTCGAA	20	7.033798E-4	45.0	9
CGCATCG	45	3.8562575E-10	45.0	21
TAGCGCG	45	3.8562575E-10	45.0	1
CCTACGG	45	3.8562575E-10	45.0	2
GCGTTGC	20	7.033798E-4	45.0	35
>>END_MODULE