FastQCFastQC Report
Thu 26 May 2016
SRR1547627_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547627_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1940168
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99000.5102650904457758No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTCGA253.8918013E-545.034
CGGTCTA1850.041.35135331
CGTTTTT55950.040.6568371
TTTCGCG800.039.3751
TAGGGAC17350.039.0345845
CGTTAGG3850.038.571432
CGACGGT1950.038.07692328
ACACCCG659.094947E-1238.0769234
TAGGGCG5650.037.831865
GACCGAT8300.037.6807259
ATAGGGC9350.037.540114
TCGGTAC601.5643309E-1037.50000436
GTCGGTC301.14021124E-437.50000439
TAAGGGA24800.037.2883074
CGAGGGA13450.037.1375474
TATGGGC7100.037.0774654
TAATGCG850.037.0588231
TACGGGA6850.036.7883224
TATGGGA21000.036.754
ATTGGGA18450.036.7073174
Produced by FastQC (version 0.11.5)