##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547627_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1940168
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.253694525422542	31.0	31.0	33.0	30.0	34.0
2	31.661438597069946	31.0	31.0	34.0	30.0	34.0
3	31.81159930480247	31.0	31.0	34.0	30.0	34.0
4	35.58651003418261	37.0	35.0	37.0	33.0	37.0
5	35.4877814704706	37.0	35.0	37.0	33.0	37.0
6	35.5230253256419	37.0	35.0	37.0	33.0	37.0
7	35.86750477278256	37.0	35.0	37.0	35.0	37.0
8	35.88875293273572	37.0	35.0	37.0	35.0	37.0
9	37.69311935873594	39.0	37.0	39.0	35.0	39.0
10	37.06620457609856	39.0	37.0	39.0	33.0	39.0
11	36.79787678180446	39.0	37.0	39.0	32.0	39.0
12	36.25280130380462	38.0	35.0	39.0	32.0	39.0
13	36.04630578382903	38.0	35.0	39.0	31.0	39.0
14	37.02273669084327	39.0	35.0	41.0	31.0	41.0
15	37.24420101764383	39.0	35.0	41.0	32.0	41.0
16	37.324860527541944	39.0	35.0	41.0	32.0	41.0
17	37.247979040990266	39.0	35.0	41.0	32.0	41.0
18	37.150251421526384	39.0	35.0	41.0	32.0	41.0
19	37.11941285496926	39.0	35.0	41.0	32.0	41.0
20	36.97791840706578	39.0	35.0	41.0	31.0	41.0
21	36.811219956209975	39.0	35.0	41.0	31.0	41.0
22	36.771151776547185	38.0	35.0	41.0	31.0	41.0
23	36.71586171919133	38.0	35.0	41.0	31.0	41.0
24	36.615892025845184	38.0	35.0	40.0	31.0	41.0
25	36.503240441033974	38.0	35.0	40.0	31.0	41.0
26	36.37604733198362	38.0	35.0	40.0	30.0	41.0
27	36.303913887869506	38.0	35.0	40.0	30.0	41.0
28	36.304882876122065	38.0	35.0	40.0	30.0	41.0
29	36.31909710911632	38.0	35.0	40.0	30.0	41.0
30	36.33265521336297	38.0	35.0	40.0	30.0	41.0
31	36.2463843337278	38.0	35.0	40.0	30.0	41.0
32	36.16960386935564	38.0	35.0	40.0	30.0	41.0
33	36.03858325670767	38.0	35.0	40.0	30.0	41.0
34	35.94880958762334	38.0	35.0	40.0	29.0	41.0
35	35.78403158901703	38.0	35.0	40.0	29.0	41.0
36	35.69311265828526	38.0	35.0	40.0	28.0	41.0
37	35.662751369984456	38.0	34.0	40.0	28.0	41.0
38	35.577769038557484	38.0	34.0	40.0	28.0	41.0
39	35.50590103537426	38.0	34.0	40.0	27.0	41.0
40	35.43108534930996	38.0	34.0	40.0	27.0	41.0
41	35.33746716779166	38.0	34.0	40.0	27.0	41.0
42	35.29933232586044	38.0	34.0	40.0	27.0	41.0
43	35.24757752936859	38.0	34.0	40.0	27.0	41.0
44	35.128688855810424	37.0	34.0	40.0	26.0	41.0
45	35.04638206588295	37.0	34.0	40.0	26.0	41.0
46	35.00435065417015	37.0	34.0	40.0	26.0	41.0
47	34.954934830385824	37.0	34.0	40.0	26.0	41.0
48	34.82180151409569	37.0	34.0	40.0	26.0	41.0
49	34.757420491421364	37.0	34.0	40.0	26.0	41.0
50	34.675781169465736	37.0	34.0	40.0	26.0	41.0
51	34.23312311098832	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	7.0
12	8.0
13	8.0
14	19.0
15	47.0
16	137.0
17	360.0
18	808.0
19	1668.0
20	2671.0
21	4293.0
22	6621.0
23	9853.0
24	14007.0
25	19909.0
26	26641.0
27	31718.0
28	36011.0
29	41156.0
30	49771.0
31	59623.0
32	74472.0
33	95232.0
34	153307.0
35	212285.0
36	145773.0
37	187943.0
38	281272.0
39	484416.0
40	131.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.178741222409606	22.713496975519647	28.96919235859987	14.138569443470875
2	30.308663991984197	25.619224726930863	30.49050391512488	13.58160736596006
3	28.719935593206365	25.540159408875933	32.13855707340807	13.60134792450963
4	25.875594278433624	28.60556405424685	30.81810441157673	14.700737255742801
5	23.329216851324215	32.13087732608722	29.693614161247893	14.846291661340668
6	21.659773792784957	40.60926682637792	27.11723933185167	10.61372004898545
7	88.85297561860622	3.4268681887341716	6.0909158382160715	1.6292403544435328
8	89.18841048816391	2.8749056782711597	5.832690777293513	2.103993056271416
9	83.87335529706705	5.33294024022662	7.850866522899048	2.9428379398072746
10	41.404043361193466	32.28070971173631	14.265259503300745	12.049987423769489
11	34.84291051084236	23.388541610829577	25.710660107784484	16.057887770543584
12	34.32187315737607	21.205740946144868	27.18156365840484	17.29082223807423
13	24.05497874410876	29.2615897180038	28.195599556327082	18.48783198156036
14	18.6342625999398	31.87620865821929	29.722735350753133	19.76679339108778
15	18.088897456302753	26.298856593861974	38.388634386300566	17.223611563534703
16	21.13801485232207	24.61297166018613	36.26041662371506	17.988596863776745
17	21.879754742888245	24.569367188820763	28.126430288511102	25.424447779779896
18	23.624449016786176	26.03279715983358	31.19369044330182	19.149063380078427
19	26.222935333435043	27.894697778749055	26.55790632563778	19.32446056217812
20	27.56354088924258	26.874528391355803	27.171203730810937	18.39072698859068
21	23.89123003781116	27.607609237962897	29.29694748083671	19.20421324338923
22	23.7070191859674	24.784090862234613	29.48456009995011	22.02432985184788
23	22.06350171737705	27.008125069581602	28.721533392984526	22.20683982005682
24	21.36443854346634	26.002902841403426	33.26072793696216	19.371930678168077
25	20.56332235146647	27.891759888834365	30.53725244411824	21.00766531558092
26	19.80168727656574	31.14415865017875	28.117822786480346	20.936331286775168
27	19.913481719108862	30.215785437137406	30.166717521369286	19.704015322384453
28	18.42459003550208	28.453309197966366	33.25851163404406	19.863589132487498
29	19.349870732843755	26.018571587615092	33.57296893877231	21.05858874076884
30	21.52236301186289	28.45459774617456	30.934176834171062	19.088862407791492
31	24.99113478832761	27.20011875260287	27.94263177209396	19.86611468697556
32	26.376478737923726	27.40035914415659	27.108064868609315	19.11509724931037
33	24.928717513122574	28.17034401144643	27.00518717966692	19.89575129576408
34	20.76315040759357	27.775635924311707	29.143816411774647	22.31739725632007
35	20.930352423089136	28.31677462982587	29.53594740249298	21.216925544592012
36	25.048552496484838	27.671624312946097	27.817333344328944	19.462489846240118
37	21.242387257185978	30.441384457428427	28.533199186874537	19.783029098511058
38	21.18533034252704	29.474251714284538	27.741824419328637	21.598593523859787
39	20.958030438601195	30.14764700788798	27.839135580011625	21.0551869734992
40	23.097896676988796	27.455972884822344	28.052158369790657	21.393972068398202
41	19.59639577603589	26.32040111990302	29.409566594233077	24.673636509828015
42	21.5752965722556	26.969520165264036	28.028191373118204	23.42699188936216
43	21.847334869970023	26.870611204802884	28.528457329468377	22.75359659575872
44	20.7885605782592	29.425647675871367	28.18348720317003	21.602304542699397
45	19.61273456731582	31.403929968951143	26.51007541614953	22.47326004758351
46	21.709563295549664	30.391646496592045	26.94637783944483	20.952412368413455
47	20.849534679471056	28.103854923903498	29.333645333806146	21.712965062819304
48	21.817749803109834	26.994930335929674	30.188004337768685	20.999315523191804
49	21.05817640534222	26.420031667360767	30.058273304167475	22.463518623129545
50	19.65979234787915	29.25370380296964	29.44817149855064	21.638332350600567
51	18.95974987733021	30.567507556046692	27.604310554549915	22.868432012073182
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	999.0
1	1734.5
2	2470.0
3	9597.5
4	16725.0
5	11843.5
6	6962.0
7	6959.0
8	6956.0
9	7283.5
10	7611.0
11	7872.0
12	8133.0
13	8034.0
14	7935.0
15	7854.5
16	7774.0
17	7383.5
18	6993.0
19	7129.5
20	7266.0
21	7554.0
22	7842.0
23	8855.5
24	9869.0
25	11655.5
26	18269.0
27	23096.0
28	26140.0
29	29184.0
30	33484.5
31	37785.0
32	44608.0
33	51431.0
34	59282.5
35	67134.0
36	70911.5
37	74689.0
38	83340.0
39	91991.0
40	105630.5
41	119270.0
42	135219.0
43	151168.0
44	156767.0
45	162366.0
46	164672.5
47	166979.0
48	163315.5
49	159652.0
50	153211.0
51	146770.0
52	133017.5
53	119265.0
54	108197.0
55	97129.0
56	89820.5
57	82512.0
58	74906.5
59	67301.0
60	61260.5
61	55220.0
62	48403.5
63	41587.0
64	35770.5
65	29954.0
66	24908.0
67	19862.0
68	16997.5
69	14133.0
70	11539.0
71	8945.0
72	7107.5
73	5270.0
74	4329.5
75	2466.0
76	1543.0
77	1190.5
78	838.0
79	609.5
80	381.0
81	267.5
82	154.0
83	144.0
84	134.0
85	86.5
86	39.0
87	22.0
88	5.0
89	5.0
90	5.0
91	4.5
92	4.0
93	4.0
94	4.0
95	3.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1940168.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.616721783169773
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.95507045389985	15.865612773522814
2	7.0455535644230265	2.9051243529225923
3	2.545143786224288	1.5741756401624836
4	1.4065603630344907	1.1599465470366548
5	0.9568639175338037	0.9863698586074169
6	0.6750554824202402	0.8350458641556927
7	0.5647470894838157	0.8150263535219897
8	0.47642037612499544	0.7857781077121704
9	0.4006565878160737	0.7434202861438307
>10	6.490918569275009	33.780992116798295
>50	2.1581138363198056	31.239813916547003
>100	0.3218619255478283	8.304576254618713
>500	0.0020226985979400892	0.2566722046775959
>1k	7.585119742275335E-4	0.23139072229160196
>5k	2.5283732474251115E-4	0.5160550012811127
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9900	0.5102650904457758	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.5462578498356844E-4	0.0	0.0	0.03850182046090854	0.0
2	1.5462578498356844E-4	0.0	0.0	0.11504158402777491	0.0
3	1.5462578498356844E-4	0.0	0.0	0.19317914737280484	0.0
4	1.5462578498356844E-4	0.0	0.0	0.35321683483079813	0.0
5	1.5462578498356844E-4	0.0	0.0	0.6186577657192573	0.0
6	1.5462578498356844E-4	0.0	0.0	1.0614544719838694	0.0
7	1.5462578498356844E-4	0.0	0.0	1.283754808861913	0.0
8	1.5462578498356844E-4	0.0	0.0	1.869941159734621	0.0
9	1.5462578498356844E-4	0.0	0.0	2.1330111619199985	0.0
10	1.5462578498356844E-4	0.0	0.0	2.514833766972757	0.0
11	1.5462578498356844E-4	0.0	0.0	2.87851361325411	0.0
12	1.5462578498356844E-4	0.0	0.0	3.1861673834430833	0.0
13	1.5462578498356844E-4	0.0	0.0	3.3308455762593754	0.0
14	1.5462578498356844E-4	0.0	0.0	3.406612210901324	0.0
15	1.5462578498356844E-4	0.0	0.0	3.487223786806091	0.0
16	1.5462578498356844E-4	0.0	0.0	3.6424165330012657	0.0
17	1.5462578498356844E-4	0.0	0.0	3.813329567336437	0.0
18	1.5462578498356844E-4	0.0	0.0	4.022486712490877	0.0
19	1.5462578498356844E-4	0.0	0.0	4.145104959982847	0.0
20	1.5462578498356844E-4	0.0	0.0	4.291484036433959	0.0
21	1.5462578498356844E-4	0.0	0.0	4.475540262492733	0.0
22	1.5462578498356844E-4	0.0	0.0	4.674028228483307	0.0
23	1.5462578498356844E-4	0.0	0.0	4.884267754132632	0.0
24	1.5462578498356844E-4	0.0	0.0	5.050748182631607	0.0
25	1.5462578498356844E-4	0.0	0.0	5.197281884867702	0.0
26	1.5462578498356844E-4	0.0	0.0	5.338094433059405	0.0
27	1.5462578498356844E-4	0.0	0.0	5.488699947633401	0.0
28	1.5462578498356844E-4	0.0	0.0	5.642037184408773	0.0
29	1.5462578498356844E-4	0.0	0.0	5.818619830860008	0.0
30	1.5462578498356844E-4	0.0	0.0	6.045919734785854	0.0
31	1.5462578498356844E-4	0.0	0.0	6.232604599189348	0.0
32	1.5462578498356844E-4	0.0	0.0	6.419186379736188	0.0
33	1.5462578498356844E-4	0.0	0.0	6.595975193900735	0.0
34	1.5462578498356844E-4	0.0	0.0	6.785649490147245	0.0
35	1.5462578498356844E-4	0.0	0.0	7.0115577620082385	0.0
36	1.5462578498356844E-4	0.0	0.0	7.207623257367403	0.0
37	1.5462578498356844E-4	0.0	0.0	7.408636777846042	0.0
38	1.5462578498356844E-4	0.0	0.0	7.659336717232734	0.0
39	1.5462578498356844E-4	0.0	0.0	8.084093748582596	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCTCGA	25	3.8918013E-5	45.0	34
CGGTCTA	185	0.0	41.351353	31
CGTTTTT	5595	0.0	40.656837	1
TTTCGCG	80	0.0	39.375	1
TAGGGAC	1735	0.0	39.034584	5
CGTTAGG	385	0.0	38.57143	2
CGACGGT	195	0.0	38.076923	28
ACACCCG	65	9.094947E-12	38.07692	34
TAGGGCG	565	0.0	37.83186	5
GACCGAT	830	0.0	37.680725	9
ATAGGGC	935	0.0	37.54011	4
TCGGTAC	60	1.5643309E-10	37.500004	36
GTCGGTC	30	1.14021124E-4	37.500004	39
TAAGGGA	2480	0.0	37.288307	4
CGAGGGA	1345	0.0	37.137547	4
TATGGGC	710	0.0	37.077465	4
TAATGCG	85	0.0	37.058823	1
TACGGGA	685	0.0	36.788322	4
TATGGGA	2100	0.0	36.75	4
ATTGGGA	1845	0.0	36.707317	4
>>END_MODULE