FastQCFastQC Report
Thu 26 May 2016
SRR1547626_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547626_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2322966
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156720.6746547302026806No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGACG302.1664637E-645.0000041
CGTTTTT97350.042.2727281
CGTAAGG4950.040.909092
TAATGCG501.0822987E-940.51
TATAGCG1450.040.3448261
ACGGGAT10550.040.0947845
GGCGATA6900.039.1304368
GGGCGAT32050.039.1029627
AGGGCGA19600.038.1122446
CGTTACA301.1402807E-437.50000438
TAGGGAT39150.037.4712645
TTAGGGA29850.037.3869324
AAGGGAT36950.037.327475
TAAGGGA29250.037.307694
GACCGAT11300.037.2345129
TATGGGA28650.037.1465954
GGCGATT10150.037.0197078
GTAGGGA33200.036.867474
CGTTAGG3300.036.8181842
TAGGGAC20950.036.5155145
Produced by FastQC (version 0.11.5)