##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547626_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2322966
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.31532704309921	31.0	31.0	33.0	30.0	34.0
2	31.726104471610864	31.0	31.0	34.0	30.0	34.0
3	31.844065733204875	31.0	31.0	34.0	30.0	34.0
4	35.65099876623248	37.0	35.0	37.0	33.0	37.0
5	35.57259081708471	37.0	35.0	37.0	33.0	37.0
6	35.62334317420057	37.0	35.0	37.0	33.0	37.0
7	35.89550557347805	37.0	35.0	37.0	35.0	37.0
8	35.910441650889425	37.0	35.0	37.0	35.0	37.0
9	37.73472749924019	39.0	37.0	39.0	35.0	39.0
10	37.15859982453467	39.0	37.0	39.0	33.0	39.0
11	36.907425679067195	39.0	37.0	39.0	33.0	39.0
12	36.48203891059964	39.0	35.0	39.0	32.0	39.0
13	36.34575280051452	39.0	35.0	39.0	32.0	39.0
14	37.39338630010082	39.0	36.0	41.0	32.0	41.0
15	37.56053510899428	40.0	36.0	41.0	32.0	41.0
16	37.60890000111926	40.0	35.0	41.0	33.0	41.0
17	37.509652315186706	39.0	35.0	41.0	32.0	41.0
18	37.39258516913291	39.0	36.0	41.0	32.0	41.0
19	37.34311694618001	39.0	36.0	41.0	32.0	41.0
20	37.202740806365654	39.0	35.0	41.0	32.0	41.0
21	37.040424181843385	39.0	35.0	41.0	32.0	41.0
22	36.996143723153935	39.0	35.0	41.0	31.0	41.0
23	36.906107967142006	39.0	35.0	41.0	31.0	41.0
24	36.83349132100943	38.0	35.0	41.0	31.0	41.0
25	36.704975879974135	38.0	35.0	40.0	31.0	41.0
26	36.613128646738694	38.0	35.0	40.0	31.0	41.0
27	36.534730598725936	38.0	35.0	40.0	31.0	41.0
28	36.533827012534836	38.0	35.0	40.0	31.0	41.0
29	36.54252924924428	38.0	35.0	40.0	31.0	41.0
30	36.53936906523815	38.0	35.0	40.0	31.0	41.0
31	36.472678033169665	38.0	35.0	41.0	30.0	41.0
32	36.342590894571856	38.0	35.0	41.0	30.0	41.0
33	36.190965773928674	38.0	35.0	41.0	30.0	41.0
34	36.06074733767089	38.0	35.0	41.0	30.0	41.0
35	35.85951710012114	38.0	35.0	41.0	29.0	41.0
36	35.76886618228592	38.0	35.0	41.0	28.0	41.0
37	35.749436711514505	38.0	35.0	41.0	28.0	41.0
38	35.65152094348346	38.0	35.0	40.0	28.0	41.0
39	35.57591157167173	38.0	35.0	40.0	27.0	41.0
40	35.49579373955538	38.0	35.0	40.0	27.0	41.0
41	35.402884932452736	38.0	34.0	40.0	27.0	41.0
42	35.36281116469203	38.0	34.0	40.0	27.0	41.0
43	35.32248211984162	38.0	34.0	40.0	27.0	41.0
44	35.19248323049067	38.0	34.0	40.0	26.0	41.0
45	35.10918627306641	38.0	34.0	40.0	26.0	41.0
46	35.08560047800958	38.0	34.0	40.0	26.0	41.0
47	35.02999785618903	38.0	34.0	40.0	26.0	41.0
48	34.88508441363326	37.0	34.0	40.0	25.0	41.0
49	34.80868897779821	37.0	34.0	40.0	25.0	41.0
50	34.72882556180331	37.0	34.0	40.0	24.0	41.0
51	34.31641444601428	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	7.0
13	12.0
14	15.0
15	52.0
16	116.0
17	319.0
18	826.0
19	1569.0
20	2871.0
21	4730.0
22	7261.0
23	11238.0
24	16568.0
25	24647.0
26	33233.0
27	38982.0
28	42430.0
29	46551.0
30	55105.0
31	66904.0
32	82893.0
33	106673.0
34	170702.0
35	235097.0
36	175464.0
37	228557.0
38	352857.0
39	617109.0
40	175.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.28956773366463	22.90842827660844	27.886288477747843	14.915715511979084
2	32.24649004763737	25.124000953952834	29.25165499624187	13.377854002167918
3	29.210629858551524	25.13304112070517	32.13925645058946	13.517072570153847
4	26.029222984753115	27.41017302879164	31.674376637454017	14.886227349001233
5	23.10503037926513	32.49294221267122	30.348614659017827	14.053412749045831
6	21.747800010848202	40.7631880965972	27.5950229146703	9.893988977884307
7	88.03783611124743	3.253125530033586	7.161792294850635	1.5472460638683476
8	88.39582671463981	2.871716589911346	6.9488317952135334	1.7836249002353026
9	82.95244097416837	5.623629446147726	8.6399887040964	2.7839408755875032
10	39.4353597943319	33.6450468926364	15.540950663935677	11.378642649096026
11	32.206067587730516	24.411851055934523	27.39424511594229	15.987836240392669
12	31.167524621539876	22.683500318127773	28.945150294924677	17.20382476540767
13	23.91038009165868	28.192147452868443	29.580501823961264	18.316970631511612
14	19.17935949127107	30.854476561430516	30.426532286740315	19.539631660558097
15	18.482750070384153	26.962555629311836	37.24036425845234	17.314330041851665
16	22.884321165268883	24.91056692177156	35.13955865044947	17.065553262510083
17	22.185645420552863	25.32499399474637	29.468532901471654	23.020827683229115
18	22.971063717678174	26.187081515614093	32.415756407971536	18.4260983587362
19	26.07369199549197	27.375605153067244	27.99761167404086	18.553091177399928
20	26.995444616925084	27.12127512843494	28.351168290883294	17.53211196375668
21	24.918616975022452	27.060619914368093	30.752107435063618	17.268655675545833
22	23.928116037858498	24.222007554135534	30.47005423239083	21.37982217561514
23	21.790331842997272	27.327433978801242	30.340607654180047	20.541626524021446
24	21.12971089546726	26.29608009759936	33.844490190558105	18.72971881637527
25	21.173189792704672	27.53979180065485	31.457068248093172	19.829950158547305
26	20.7664683856759	31.06468196262881	28.268472289306	19.90037736238929
27	19.85866345008924	30.28593616953498	30.95581252588286	18.899587854492918
28	18.877805357461106	28.538644129961437	33.549350270300984	19.03420024227647
29	19.615095528733526	26.551529380972426	33.708069769424085	20.125305320869955
30	21.06948616553148	28.04087532921274	31.898960208629827	18.99067829662595
31	24.222351941440383	27.140345575441057	29.442789950434054	19.194512532684506
32	25.13019992544015	27.099492631403127	29.044118596656173	18.726188846500552
33	24.248353182956617	27.77518052352036	28.347982708313424	19.6284835852096
34	20.33662998080902	28.195935713221804	30.26957777255457	21.19785653341461
35	20.769783113485087	28.796891560186417	30.426790577218952	20.006534749109544
36	23.95992881514409	28.657500798548064	28.1979589886378	19.184611397670047
37	21.11511748342421	30.635919768089586	28.9710654396147	19.277897308871502
38	21.113955176270338	30.560800287219013	27.77530966875968	20.54993486775097
39	20.78321421837427	30.08356557952204	28.667961562932902	20.465258639170784
40	23.157075910710702	28.018748444876078	28.10760036952758	20.716575274885642
41	19.847556959507802	27.289723568920078	29.43521342972734	23.42750604184478
42	21.35274472377125	27.636995117448986	28.423532673315066	22.586727485464703
43	21.65266301788317	27.41301422405666	28.670372274066864	22.26395048399331
44	20.66298000056824	29.27524552662415	29.086650428805243	20.975124044002367
45	19.949108166025674	31.150477449949765	27.2579968884607	21.642417495563862
46	21.253518131561115	30.27220372575406	28.090122713806405	20.384155428878426
47	20.832633796620357	28.465763166572387	29.48334155558024	21.218261481227017
48	21.326140804471525	27.695842298165363	30.647112355497242	20.330904541865873
49	21.489724774275647	27.082445459813016	30.23341710554524	21.1944126603661
50	20.075240016427273	29.785799706065436	29.610549616309495	20.528410661197796
51	19.582249589533383	29.969185945898474	28.723924499971158	21.724639964596985
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1103.0
1	2035.5
2	2968.0
3	15272.0
4	27576.0
5	19685.5
6	11795.0
7	11520.5
8	11246.0
9	11689.5
10	12133.0
11	12193.5
12	12254.0
13	12098.5
14	11943.0
15	11776.5
16	11610.0
17	11203.5
18	10797.0
19	11007.0
20	11217.0
21	11402.5
22	11588.0
23	12408.5
24	13229.0
25	16587.5
26	24885.0
27	29824.0
28	33863.0
29	37902.0
30	44766.5
31	51631.0
32	59312.0
33	66993.0
34	75553.0
35	84113.0
36	91233.5
37	98354.0
38	106960.0
39	115566.0
40	129565.5
41	143565.0
42	158199.0
43	172833.0
44	178528.0
45	184223.0
46	184262.0
47	184301.0
48	180813.0
49	177325.0
50	169772.0
51	162219.0
52	153121.5
53	144024.0
54	131268.0
55	118512.0
56	107032.0
57	95552.0
58	87940.0
59	80328.0
60	71662.5
61	62997.0
62	54541.0
63	46085.0
64	39581.5
65	33078.0
66	28263.0
67	23448.0
68	19607.5
69	15767.0
70	12877.0
71	9987.0
72	8286.0
73	6585.0
74	5321.0
75	3206.0
76	2355.0
77	1781.0
78	1207.0
79	834.5
80	462.0
81	305.0
82	148.0
83	108.5
84	69.0
85	51.0
86	33.0
87	23.0
88	13.0
89	6.5
90	0.0
91	0.5
92	1.0
93	2.5
94	4.0
95	2.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2322966.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.048491432712687
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.41930174308645	14.556724145470682
2	6.413079012579232	2.443189612568501
3	2.442749106074451	1.3959205625797733
4	1.328412527395794	1.0121701858882797
5	0.9402212588336522	0.895489829687358
6	0.7219954721298107	0.8251754739193232
7	0.5864678231560635	0.7819929113464956
8	0.4816106150627309	0.7339164539940735
9	0.44796615670391415	0.7679771548307764
>10	7.511942621561785	36.09276965503208
>50	2.37819320828926	31.647304814616472
>100	0.32553867955884713	7.758174274274497
>500	0.0016047662705342826	0.19799574186271438
>1k	6.877569730861211E-4	0.20681690195452476
>5k	0.0	0.0
>10k+	2.292523243620404E-4	0.684382281974517
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15672	0.6746547302026806	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.304841310634766E-5	0.0	0.0	0.01709022000322002	0.0
2	4.304841310634766E-5	0.0	0.0	0.04855860998396016	0.0
3	4.304841310634766E-5	0.0	0.0	0.08691474606171593	0.0
4	4.304841310634766E-5	0.0	0.0	0.1555339165532341	0.0
5	4.304841310634766E-5	0.0	0.0	0.272410358136968	0.0
6	4.304841310634766E-5	0.0	0.0	0.5136536651849403	0.0
7	4.304841310634766E-5	0.0	0.0	0.6448652283330879	0.0
8	4.304841310634766E-5	0.0	0.0	0.9712152480923095	0.0
9	4.304841310634766E-5	0.0	0.0	1.1369085901386418	0.0
10	4.304841310634766E-5	0.0	0.0	1.3617074033799892	0.0
11	4.304841310634766E-5	0.0	0.0	1.561279846541017	0.0
12	4.304841310634766E-5	0.0	0.0	1.7301157227441124	0.0
13	4.304841310634766E-5	0.0	0.0	1.8220240847261648	0.0
14	4.304841310634766E-5	0.0	0.0	1.8745001003028026	0.0
15	4.304841310634766E-5	0.0	0.0	1.9226282261556993	0.0
16	4.304841310634766E-5	0.0	0.0	2.006271292821333	0.0
17	4.304841310634766E-5	0.0	0.0	2.0976630738461086	0.0
18	4.304841310634766E-5	0.0	0.0	2.2111817392075475	0.0
19	4.304841310634766E-5	0.0	0.0	2.285397203402891	0.0
20	4.304841310634766E-5	0.0	0.0	2.3700303835699703	0.0
21	4.304841310634766E-5	0.0	0.0	2.4722273162844397	0.0
22	4.304841310634766E-5	0.0	0.0	2.5823882054235834	0.0
23	4.304841310634766E-5	0.0	0.0	2.7039138756228027	0.0
24	4.304841310634766E-5	0.0	0.0	2.8039583876819547	0.0
25	4.304841310634766E-5	0.0	0.0	2.891303617874734	0.0
26	4.304841310634766E-5	0.0	0.0	2.977056056782579	0.0
27	4.304841310634766E-5	0.0	0.0	3.0681464989156106	0.0
28	4.304841310634766E-5	0.0	0.0	3.165048476817999	0.0
29	4.304841310634766E-5	0.0	0.0	3.2851965977978153	0.0
30	4.304841310634766E-5	0.0	0.0	3.4324652190346305	0.0
31	4.304841310634766E-5	0.0	0.0	3.5570903749775074	0.0
32	4.304841310634766E-5	0.0	0.0	3.683824903162595	0.0
33	4.304841310634766E-5	0.0	0.0	3.8104733345214696	0.0
34	4.304841310634766E-5	0.0	0.0	3.9454301096098696	0.0
35	8.609682621269532E-5	0.0	0.0	4.089556196689921	0.0
36	8.609682621269532E-5	0.0	0.0	4.229549636111764	0.0
37	8.609682621269532E-5	0.0	0.0	4.372986948582114	0.0
38	8.609682621269532E-5	0.0	0.0	4.54901190977397	0.0
39	8.609682621269532E-5	0.0	0.0	4.832227419600631	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGGACG	30	2.1664637E-6	45.000004	1
CGTTTTT	9735	0.0	42.272728	1
CGTAAGG	495	0.0	40.90909	2
TAATGCG	50	1.0822987E-9	40.5	1
TATAGCG	145	0.0	40.344826	1
ACGGGAT	1055	0.0	40.094784	5
GGCGATA	690	0.0	39.130436	8
GGGCGAT	3205	0.0	39.102962	7
AGGGCGA	1960	0.0	38.112244	6
CGTTACA	30	1.1402807E-4	37.500004	38
TAGGGAT	3915	0.0	37.471264	5
TTAGGGA	2985	0.0	37.386932	4
AAGGGAT	3695	0.0	37.32747	5
TAAGGGA	2925	0.0	37.30769	4
GACCGAT	1130	0.0	37.234512	9
TATGGGA	2865	0.0	37.146595	4
GGCGATT	1015	0.0	37.019707	8
GTAGGGA	3320	0.0	36.86747	4
CGTTAGG	330	0.0	36.818184	2
TAGGGAC	2095	0.0	36.515514	5
>>END_MODULE