FastQCFastQC Report
Thu 26 May 2016
SRR1547625_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547625_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1735013
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99830.5753847377512445No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC69450.40028518518305056No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT47980.27653971468801675No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCTA302.1661435E-645.00000422
AGTCCGA302.1661435E-645.00000436
ACGCCAA207.0342806E-445.00000441
TTGTGCG800.045.0000041
CGACGAA207.0342806E-445.00000417
ACGTTAG600.045.0000041
CGTTGCC207.0342806E-445.00000440
GGTCCGT302.1661435E-645.0000049
TCGATGC207.0342806E-445.0000041
AAACGTC207.0342806E-445.00000428
CCCGTAG207.0342806E-445.0000041
CGCTCCG406.8193913E-945.00000428
GTATCGC207.0342806E-445.00000424
ACGCTAT600.045.00000415
ACGCTAG207.0342806E-445.00000435
TTACGCC207.0342806E-445.00000439
CGTCGAC207.0342806E-445.00000443
CCGGTAT207.0342806E-445.00000411
ACCGTAT302.1661435E-645.00000410
ATCGGAC207.0342806E-445.00000439