FastQCFastQC Report
Thu 26 May 2016
SRR1547624_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547624_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1130588
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67520.5972113625830099No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC43370.383605698981415No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGAA207.0332363E-445.00000419
ACGCCAG207.0332363E-445.00000418
TTAGCGT207.0332363E-445.00000422
AGCCCCC207.0332363E-445.00000445
TGTGGCG207.0332363E-445.00000414
ACCGACT207.0332363E-445.00000425
TTCGTAG750.045.0000041
TCGATAG650.045.0000041
CTGCGTA207.0332363E-445.00000441
TACATGC207.0332363E-445.00000427
CGTTACA207.0332363E-445.00000420
TATTGAC207.0332363E-445.00000442
AATGCGT406.8157533E-945.00000414
GCCGACG207.0332363E-445.0000041
TCTACGT207.0332363E-445.00000434
CACTAGT207.0332363E-445.00000423
TCGTCGG207.0332363E-445.00000444
TGTTACG207.0332363E-445.0000041
ACGTCGT207.0332363E-445.00000434
GTATACT207.0332363E-445.00000413