FastQCFastQC Report
Thu 26 May 2016
SRR1547623_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547623_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2678654
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT355671.3277937352117892No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT172250.043.105951
CGGTCTA1750.041.14285731
CGACGGT1750.039.85714328
GCGCGAC1650.039.5454529
CGAGGGA18000.039.1254
TCTCGCG3050.039.09836232
CTCGCGC3050.039.09836233
CGTATGG2650.039.0566022
GCGATAC1500.039.09
CGCGATA356.2514027E-638.5714311
AGGGCGA19400.038.3891756
CGCGACC1700.038.38235510
TACGGGA12850.038.3463024
ACGGGTC1300.038.076925
TCGCGCA3150.037.8571434
CGACCCG1550.037.7419432
ACGGGAT15800.037.737345
ACGGTCT1850.037.702730
AGGGATC41500.037.6265076
GGGCGAT39050.037.50967
Produced by FastQC (version 0.11.5)