FastQCFastQC Report
Thu 26 May 2016
SRR1547622_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547622_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2522308
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108940.43190601623592356No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT54790.21722168743864745No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC35700.1415370367139937No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATCGA207.0348906E-445.00000421
CGTACGT351.212702E-745.038
GCTACGA6700.041.97760810
CGTAAGG2550.041.470592
CGGTCTA4150.041.2048231
CGTTTTT72550.041.0613371
GCGTTAG2650.040.754721
CGTTAGG4200.040.7142872
TAATGCG2400.040.3125041
CACGACG4350.039.82758726
CGACGGT4300.039.7674428
TGTAGCG2800.039.3751
CGAACCC1900.039.07894519
CTAAGCG1100.038.8636361
ATAACGG2100.038.571432
TTGCGAG1750.038.5714261
ACGGGTA3800.038.4868435
GGCGATA8450.038.3431938
AGGGATG51300.038.2456176
TAACGGG7950.038.207553
Produced by FastQC (version 0.11.5)