##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547622_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2522308
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.26357724750506	31.0	31.0	33.0	30.0	34.0
2	31.667844291815275	31.0	31.0	34.0	30.0	34.0
3	31.81294195633523	31.0	31.0	34.0	30.0	34.0
4	35.57833737989175	37.0	35.0	37.0	33.0	37.0
5	35.475039130827795	37.0	35.0	37.0	33.0	37.0
6	35.51925538038971	37.0	35.0	37.0	33.0	37.0
7	35.86908617028531	37.0	35.0	37.0	35.0	37.0
8	35.8894556097035	37.0	35.0	37.0	35.0	37.0
9	37.69597170527945	39.0	37.0	39.0	35.0	39.0
10	37.0690934651914	39.0	37.0	39.0	33.0	39.0
11	36.788189229864074	39.0	37.0	39.0	32.0	39.0
12	36.17036658488971	38.0	35.0	39.0	32.0	39.0
13	35.932828187517146	38.0	35.0	39.0	31.0	39.0
14	36.94129543259586	39.0	35.0	41.0	31.0	41.0
15	37.159566555710086	39.0	35.0	41.0	32.0	41.0
16	37.25195138738013	39.0	35.0	41.0	32.0	41.0
17	37.16384557318139	39.0	35.0	41.0	32.0	41.0
18	37.08670075185108	39.0	35.0	41.0	32.0	41.0
19	37.05699185032121	38.0	35.0	41.0	32.0	41.0
20	36.899969789573674	38.0	35.0	41.0	32.0	41.0
21	36.72018484657703	38.0	35.0	41.0	31.0	41.0
22	36.65892507972857	38.0	35.0	41.0	31.0	41.0
23	36.603660219132635	38.0	35.0	40.0	31.0	41.0
24	36.51072906243012	38.0	35.0	40.0	31.0	41.0
25	36.41499134919288	38.0	35.0	40.0	31.0	41.0
26	36.29406916205317	38.0	35.0	40.0	30.0	41.0
27	36.21887771041443	38.0	35.0	40.0	30.0	41.0
28	36.23649094400842	38.0	35.0	40.0	30.0	41.0
29	36.271500149862746	38.0	35.0	40.0	30.0	41.0
30	36.298501213967526	38.0	35.0	40.0	30.0	41.0
31	36.20968890397208	38.0	35.0	40.0	30.0	41.0
32	36.089449821354094	38.0	35.0	40.0	30.0	41.0
33	35.963688811992824	38.0	35.0	40.0	30.0	41.0
34	35.878700777224665	38.0	35.0	40.0	29.0	41.0
35	35.733011590971444	38.0	35.0	40.0	29.0	41.0
36	35.625453354626	38.0	35.0	40.0	28.0	41.0
37	35.587329541039395	38.0	34.0	40.0	28.0	41.0
38	35.514026042814756	38.0	34.0	40.0	28.0	41.0
39	35.46474538398959	38.0	34.0	40.0	27.0	41.0
40	35.357057900938344	38.0	34.0	40.0	27.0	41.0
41	35.28400219164352	38.0	34.0	40.0	27.0	41.0
42	35.258189325015024	38.0	34.0	40.0	27.0	41.0
43	35.205024921619405	38.0	34.0	40.0	27.0	41.0
44	35.08725143796872	37.0	34.0	40.0	27.0	41.0
45	35.01213412477778	37.0	34.0	40.0	26.0	41.0
46	34.973392226484634	37.0	34.0	40.0	26.0	41.0
47	34.93450839469248	37.0	34.0	40.0	26.0	41.0
48	34.82166650543867	37.0	34.0	40.0	26.0	41.0
49	34.759356509990056	37.0	34.0	40.0	26.0	41.0
50	34.67638250364349	37.0	34.0	40.0	26.0	41.0
51	34.251441933340416	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	3.0
10	3.0
11	2.0
12	2.0
13	9.0
14	20.0
15	60.0
16	190.0
17	461.0
18	992.0
19	2057.0
20	3500.0
21	5732.0
22	8839.0
23	13041.0
24	18845.0
25	26551.0
26	35266.0
27	41742.0
28	46636.0
29	53678.0
30	64306.0
31	77720.0
32	96365.0
33	124472.0
34	205394.0
35	307790.0
36	179019.0
37	228596.0
38	349485.0
39	631340.0
40	189.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.71538765289568	23.53792637536732	28.426544260256875	13.32014171148012
2	30.67884651676163	26.140899525355348	29.671515136137224	13.508738821745798
3	29.081420667103302	25.635330816062112	31.223506407623493	14.059742109211088
4	25.89727344955493	28.598569246896098	30.81578459093814	14.688372712610832
5	23.074343022343026	32.36833090962721	29.765714575698134	14.791611492331628
6	21.636691474633547	41.048317651928315	27.351972875636122	9.963017997802014
7	88.37406058260926	3.7017287341593494	6.2234271151659515	1.7007835680654386
8	88.77559758760627	3.2335860648263415	6.0202798389411605	1.9705365086262263
9	83.53492119122646	6.060600053601702	7.696680976312171	2.707797778859679
10	42.863678821143175	31.134579916489187	14.557222987834951	11.44451827453269
11	36.255048947234044	23.754156907086685	24.837926216782407	15.152867928896868
12	36.033386882173	22.130088791693954	26.227288657848284	15.609235668284763
13	22.991958158956006	34.207400523647394	25.940131022856843	16.86051029453976
14	17.668381498215126	36.52587233597166	27.559917345542257	18.245828820270958
15	16.825899136822304	27.49101219993752	39.5248320189287	16.158256644311482
16	19.45067771263462	23.960158711783016	39.30301136895256	17.2861522066298
17	19.34735964045628	25.00091186326174	28.197904458932054	27.453824037349918
18	21.82568504718694	26.480231597409993	32.26374415812819	19.430339197274876
19	25.984257275479443	28.27489743520617	26.357764396735057	19.383080892579336
20	28.295156658108368	25.806681816812223	27.943692840049668	17.95446868502974
21	23.24977758465659	28.248334461929314	29.38748955321872	19.114398400195377
22	22.749759347391358	25.58145159116175	28.287306704811627	23.38148235663527
23	20.42966203968746	29.450249533363888	28.092009381883575	22.028079045065073
24	19.86950047337597	25.805888892236794	35.44912040876847	18.87549022561876
25	18.89523404754693	28.16935917421663	31.88587595170772	21.04953082652872
26	18.59019596337957	33.755988562855926	27.499456846665833	20.154358627098674
27	18.173950207508362	33.253314028263006	29.765437052096733	18.807298712131903
28	16.64642065917406	29.82562002737176	34.623606633289825	18.904352680164358
29	17.33979355415754	26.535974195062618	34.965793233816015	21.15843901696383
30	19.522833848998616	30.619178942460636	30.828074921857286	19.029912286683466
31	24.840899683940265	29.245992162733497	27.185379422338592	18.72772873098765
32	26.217297808197888	28.238224673592597	26.80398270155746	18.74049481665205
33	24.437142490132054	28.932271554465196	26.815757631502574	19.814828323900173
34	19.183541423172745	29.29309188251395	28.41659305683525	23.106773637478057
35	19.78671914770123	27.579819752385514	30.91589131858599	21.717569781327263
36	25.53518444218549	28.007285390999037	27.435031724912264	19.02249844190321
37	19.871839600873486	31.605339236921104	30.302564159492018	18.22025700271339
38	19.51351698523733	31.374756770386487	27.314864005506067	21.796862238870116
39	19.916877716757828	31.216449378902176	28.757550624269516	20.109122280070473
40	23.299652540451046	28.370643077689163	27.437251913723465	20.892452468136327
41	18.253361603737527	26.947779573311426	30.0761445469784	24.722714275972642
42	21.430531085022132	26.054946501378893	27.937864844420268	24.576657569178707
43	21.324001668313304	27.80120429384516	27.525663003883743	23.349131033957786
44	19.54388599647624	30.444933767010212	28.564949244897925	21.446230991615618
45	18.35097061897278	33.9584618531916	25.85362295167759	21.836944576158025
46	20.931028248730925	32.6322558545586	26.122781198806805	20.313934697903665
47	19.885596842257172	29.470270878893455	28.942024526742966	21.702107752106404
48	21.341208131600105	26.552585964917846	31.196943434346636	20.909262469135413
49	20.093739543307162	26.985324551957966	30.85158513551874	22.069350769216133
50	18.681421935782623	31.278575019387006	29.70021900576773	20.339784039062636
51	18.026149066648482	31.778117501906983	27.84917623065859	22.346557200785945
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1183.0
1	2379.0
2	3575.0
3	11339.0
4	19103.0
5	14085.0
6	9067.0
7	9278.0
8	9489.0
9	9994.5
10	10500.0
11	10620.5
12	10741.0
13	10807.0
14	10873.0
15	10515.5
16	10158.0
17	9405.5
18	8653.0
19	9016.0
20	9379.0
21	10218.5
22	11058.0
23	12465.0
24	13872.0
25	18008.0
26	25005.0
27	27866.0
28	32629.5
29	37393.0
30	47506.0
31	57619.0
32	66035.5
33	74452.0
34	85054.0
35	95656.0
36	101971.0
37	108286.0
38	121375.0
39	134464.0
40	155268.0
41	176072.0
42	204060.5
43	232049.0
44	230387.0
45	228725.0
46	225424.5
47	222124.0
48	212798.5
49	203473.0
50	189858.5
51	176244.0
52	162421.5
53	148599.0
54	130002.5
55	111406.0
56	100831.0
57	90256.0
58	80426.0
59	70596.0
60	63768.5
61	56941.0
62	48742.0
63	40543.0
64	34546.5
65	28550.0
66	23674.0
67	18798.0
68	15977.0
69	13156.0
70	10328.5
71	7501.0
72	6271.5
73	5042.0
74	4253.5
75	2551.5
76	1638.0
77	1234.0
78	830.0
79	604.5
80	379.0
81	255.0
82	131.0
83	139.0
84	147.0
85	94.0
86	41.0
87	33.0
88	25.0
89	28.5
90	32.0
91	19.5
92	7.0
93	5.5
94	4.0
95	3.0
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2522308.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.699206392015192
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.62382476571084	13.915793018579514
2	8.059244074347797	2.8528444847101415
3	2.694307652479371	1.43061321674455
4	1.330833275921208	0.9421877129556465
5	0.7689082666461573	0.6804533053948494
6	0.5443070011769712	0.5780281172670041
7	0.42013994509650504	0.5205300521255081
8	0.33737391481281265	0.47770004396432975
9	0.2620139938664829	0.4173695779535174
>10	3.74993144635896	16.768753646182443
>50	1.7198440778843451	21.968674647313122
>100	1.4844997360836028	38.15922929317622
>500	0.003862925878828048	0.4272365788962067
>1k	4.544618680974174E-4	0.20209581181901895
>5k	2.272309340487087E-4	0.2203548165087373
>10k+	2.272309340487087E-4	0.43813567640923234
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10894	0.43190601623592356	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	5479	0.21722168743864745	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3570	0.1415370367139937	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.028347053571570167	0.0
2	0.0	0.0	0.0	0.09427873201845294	0.0
3	0.0	0.0	0.0	0.15180540996579323	0.0
4	0.0	0.0	0.0	0.2998047819695295	0.0
5	0.0	0.0	0.0	0.5246781915610623	0.0
6	0.0	0.0	0.0	0.8570721735807046	0.0
7	0.0	0.0	0.0	1.0147848716334404	0.0
8	0.0	0.0	0.0	1.44157652435785	0.0
9	0.0	0.0	0.0	1.5894173114465007	0.0
10	0.0	0.0	0.0	1.8579808651441458	0.0
11	0.0	0.0	0.0	2.154772533727047	0.0
12	0.0	0.0	0.0	2.4203229740380636	0.0
13	0.0	0.0	0.0	2.5320460467159442	0.0
14	0.0	0.0	0.0	2.5806523231897134	0.0
15	0.0	0.0	0.0	2.6459496619762537	0.0
16	0.0	0.0	0.0	2.796288161477504	0.0
17	0.0	0.0	0.0	2.958361944695097	0.0
18	0.0	0.0	0.0	3.166742523117716	0.0
19	0.0	0.0	0.0	3.282192341300111	0.0
20	0.0	0.0	0.0	3.411795863153905	0.0
21	0.0	0.0	0.0	3.5834243875054117	0.0
22	0.0	0.0	0.0	3.7706735259928603	0.0
23	0.0	0.0	0.0	3.96961830196788	0.0
24	0.0	0.0	0.0	4.121701235535073	0.0
25	0.0	0.0	0.0	4.25610195107021	0.0
26	0.0	0.0	0.0	4.38419891623069	0.0
27	0.0	0.0	0.0	4.511384018129427	0.0
28	0.0	0.0	0.0	4.653238224673593	0.0
29	0.0	0.0	0.0	4.810514814209843	0.0
30	0.0	0.0	0.0	5.014851477297777	0.0
31	3.96462287714268E-5	0.0	0.0	5.190087808467483	0.0
32	7.92924575428536E-5	0.0	0.0	5.357434540111676	0.0
33	7.92924575428536E-5	0.0	0.0	5.529538819208439	0.0
34	7.92924575428536E-5	0.0	0.0	5.714012721681888	0.0
35	7.92924575428536E-5	0.0	0.0	5.943881556098621	0.0
36	7.92924575428536E-5	0.0	0.0	6.140526850804898	0.0
37	7.92924575428536E-5	0.0	0.0	6.33899587203466	0.0
38	7.92924575428536E-5	0.0	0.0	6.546385294737994	0.0
39	7.92924575428536E-5	0.0	0.0	6.818794532626468	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAATCGA	20	7.0348906E-4	45.000004	21
CGTACGT	35	1.212702E-7	45.0	38
GCTACGA	670	0.0	41.977608	10
CGTAAGG	255	0.0	41.47059	2
CGGTCTA	415	0.0	41.20482	31
CGTTTTT	7255	0.0	41.061337	1
GCGTTAG	265	0.0	40.75472	1
CGTTAGG	420	0.0	40.714287	2
TAATGCG	240	0.0	40.312504	1
CACGACG	435	0.0	39.827587	26
CGACGGT	430	0.0	39.76744	28
TGTAGCG	280	0.0	39.375	1
CGAACCC	190	0.0	39.078945	19
CTAAGCG	110	0.0	38.863636	1
ATAACGG	210	0.0	38.57143	2
TTGCGAG	175	0.0	38.571426	1
ACGGGTA	380	0.0	38.486843	5
GGCGATA	845	0.0	38.343193	8
AGGGATG	5130	0.0	38.245617	6
TAACGGG	795	0.0	38.20755	3
>>END_MODULE