FastQCFastQC Report
Thu 26 May 2016
SRR1547621_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547621_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2931986
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154520.5270147947500432No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT97650.041.451611
TATCACG603.6379788E-1241.2500041
TACGGGA12450.040.1204834
CGGGATC7600.039.9671066
ACGGGTA1600.039.3755
GTTAGCG1800.038.7500041
ACGGGAT12600.038.7499965
ACACGAC4950.038.63636426
AGGGCGA22800.037.7960556
TAAGGGA35700.037.5630264
TTAACGC301.1403536E-437.50000442
CGAGGGA20400.037.54
AATGCGG4150.037.409642
TAGGGCG9050.037.2928165
GTACGGG9650.037.072543
AACGGGA11650.036.8884124
TAACGGG9100.036.840663
ATAGGGA41050.036.8331264
GGGCGAT43700.036.81357
AGGGATT53900.036.567726
Produced by FastQC (version 0.11.5)