##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547621_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2931986
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.313068684502586	31.0	31.0	33.0	30.0	34.0
2	31.724247319052683	31.0	31.0	34.0	30.0	34.0
3	31.848308961911822	31.0	31.0	34.0	30.0	34.0
4	35.623052770374755	37.0	35.0	37.0	33.0	37.0
5	35.53471264869614	37.0	35.0	37.0	33.0	37.0
6	35.57854710083882	37.0	35.0	37.0	33.0	37.0
7	35.92051496835251	37.0	35.0	37.0	35.0	37.0
8	35.94029200685133	37.0	35.0	37.0	35.0	37.0
9	37.75268060625119	39.0	37.0	39.0	35.0	39.0
10	37.15380905638703	39.0	37.0	39.0	33.0	39.0
11	36.87665937013342	39.0	37.0	39.0	32.0	39.0
12	36.38607005626903	38.0	35.0	39.0	32.0	39.0
13	36.21857164393009	38.0	35.0	39.0	32.0	39.0
14	37.22249014831585	39.0	35.0	41.0	32.0	41.0
15	37.42289833580379	39.0	35.0	41.0	32.0	41.0
16	37.489497562403095	39.0	35.0	41.0	32.0	41.0
17	37.38900901982479	39.0	35.0	41.0	32.0	41.0
18	37.292764699422165	39.0	35.0	41.0	32.0	41.0
19	37.25549473974296	39.0	35.0	41.0	32.0	41.0
20	37.11955138939954	39.0	35.0	41.0	32.0	41.0
21	36.96112498490784	39.0	35.0	41.0	31.0	41.0
22	36.918775533034605	39.0	35.0	41.0	31.0	41.0
23	36.85137343766307	38.0	35.0	41.0	31.0	41.0
24	36.77509544724975	38.0	35.0	41.0	31.0	41.0
25	36.66759288755131	38.0	35.0	40.0	31.0	41.0
26	36.54915473675522	38.0	35.0	40.0	31.0	41.0
27	36.46806396756328	38.0	35.0	40.0	31.0	41.0
28	36.484734238158026	38.0	35.0	40.0	31.0	41.0
29	36.48780860481598	38.0	35.0	40.0	31.0	41.0
30	36.49437582580544	38.0	35.0	40.0	30.0	41.0
31	36.414664326500876	38.0	35.0	41.0	30.0	41.0
32	36.31284903816048	38.0	35.0	41.0	30.0	41.0
33	36.20098015474835	38.0	35.0	41.0	30.0	41.0
34	36.10127197060286	38.0	35.0	41.0	30.0	41.0
35	35.92054395894114	38.0	35.0	41.0	29.0	41.0
36	35.81342543927563	38.0	35.0	41.0	29.0	41.0
37	35.77793311427817	38.0	35.0	41.0	29.0	41.0
38	35.67756326258038	38.0	35.0	40.0	28.0	41.0
39	35.603695924878224	38.0	35.0	40.0	27.0	41.0
40	35.522685306137205	38.0	34.0	40.0	27.0	41.0
41	35.43678687415288	38.0	34.0	40.0	27.0	41.0
42	35.402361402817064	38.0	34.0	40.0	27.0	41.0
43	35.36818627374073	38.0	34.0	40.0	27.0	41.0
44	35.23814677150573	38.0	34.0	40.0	27.0	41.0
45	35.14116302056013	38.0	34.0	40.0	26.0	41.0
46	35.11705137746224	38.0	34.0	40.0	26.0	41.0
47	35.07601912151013	37.0	34.0	40.0	26.0	41.0
48	34.954127680009385	37.0	34.0	40.0	26.0	41.0
49	34.87259284321276	37.0	34.0	40.0	26.0	41.0
50	34.80106760400629	37.0	34.0	40.0	26.0	41.0
51	34.35579262656779	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	6.0
10	6.0
11	5.0
12	7.0
13	11.0
14	28.0
15	74.0
16	200.0
17	475.0
18	1053.0
19	2067.0
20	3689.0
21	6035.0
22	9440.0
23	14170.0
24	20930.0
25	29681.0
26	39890.0
27	46972.0
28	52621.0
29	59796.0
30	71515.0
31	86660.0
32	107164.0
33	137907.0
34	219637.0
35	313577.0
36	218134.0
37	281401.0
38	431591.0
39	776963.0
40	280.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.225475155747674	23.35451124255027	27.55725982320516	13.862753778496895
2	32.02569862202616	25.50165655634099	28.990008819960263	13.482636001672589
3	29.4576781744524	25.753670037987902	31.14626058923883	13.642391198320865
4	25.95476240336755	28.597953741934646	30.71501705669809	14.732266797999719
5	23.565119342316095	32.600462621581414	29.239464308492607	14.594953727609886
6	21.86002934529701	41.03344968222904	26.97816428864258	10.128356683831369
7	88.69687645166108	3.3391359986029947	6.365889878055351	1.5980976716805606
8	88.94043150274251	3.038077262306164	6.174142714187584	1.8473485207637415
9	83.77574108471187	5.615886296864992	7.9773573270813705	2.631015291341773
10	40.110355233619806	36.147546407111086	13.564832847087263	10.177265512181846
11	32.643812078229566	24.326582732659705	27.364625888391007	15.664979300719716
12	32.151927055586214	23.011092140276247	28.73990530650556	16.09707549763198
13	23.441107836122	30.10525971133559	29.621253307485095	16.83237914505731
14	18.574474775800432	32.90636449150849	30.58561671167598	17.93354402101511
15	17.56062273148644	26.92795941044739	38.59864951606181	16.912768342004362
16	21.81852164369134	25.22716684186077	35.95839134293274	16.995920171515145
17	21.396589206087615	24.793604062229495	29.59717406563333	24.212632666049565
18	23.241379733736792	26.018507591782498	32.42761050018657	18.31250217429415
19	25.772428654161377	27.254359331865842	28.244200347477783	18.729011666494998
20	27.27833625399303	27.29538954142346	28.095154615335815	17.3311195892477
21	24.481733541701768	27.453337089604112	30.38742340515951	17.677505963534614
22	23.701920814083014	24.361371439017784	30.39710967241999	21.539598074479212
23	20.562342384990924	28.253716081863967	29.566955640306603	21.616985892838507
24	20.437000722377256	26.64190756708934	34.537238581630334	18.38385312890307
25	20.222095194178962	28.497714518418572	31.449092867428423	19.83109741997404
26	19.67110347730173	32.07051466139333	28.463164558084518	19.79521730322041
27	18.825396847051792	30.768769018678803	31.43701913992768	18.96881499434172
28	18.281840363494233	29.02241006607808	33.881983065403446	18.81376650502424
29	18.82212261586515	26.847877172674085	34.05821173770953	20.27178847375124
30	20.30030157033492	29.140043642773193	31.66055363156577	18.899101155326118
31	24.64599080623168	27.569674616454513	29.0992521792396	18.685082398074208
32	25.033577922950517	27.64798331233505	28.41541535327931	18.903023411435115
33	24.10710692343006	28.571589359567202	27.733965987559284	19.587337729443455
34	20.04115981454209	28.648124513554972	29.63718107794512	21.673534593957815
35	21.143927699518347	28.450749764835166	30.38285312412815	20.022469411518337
36	25.0233118439174	28.182740299578512	28.21125339616219	18.582694460341898
37	21.052419759166654	30.622758771699456	29.400413235261013	18.924408233872878
38	21.05859304921647	30.617642785470327	28.025577202619655	20.298186962693546
39	20.488126478093687	30.582342480489334	28.70729942093857	20.222231620478407
40	23.440357491475062	28.038128422168455	28.257501911673522	20.264012174682964
41	19.823355227480622	26.998594126984237	29.499015343183764	23.679035302351377
42	21.89567071602661	27.217012632393196	27.9099900204162	22.977326631163997
43	21.443656279395604	27.514626604629083	28.63206031679551	22.409656799179807
44	20.2884665888582	29.810237838789135	29.010404551727053	20.89089102062561
45	19.376524990228468	32.32327848768718	27.078539938458096	21.221656583626253
46	21.581242202384324	30.425793301877974	27.668754216425317	20.324210279312386
47	20.441639216558332	28.846761205544635	29.45696193638032	21.254637641516705
48	21.463915584862956	27.573392233114348	30.738038994729173	20.22465318729353
49	20.993585917531664	26.901015216307307	30.602669999106407	21.502728867054618
50	19.56892017901859	30.406761833105616	29.69123999909959	20.333077988776207
51	19.40128636357745	30.863278337618254	27.98891263464423	21.74652266416006
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1532.0
1	2655.5
2	3779.0
3	15254.5
4	26730.0
5	19270.0
6	11810.0
7	11707.0
8	11604.0
9	12275.5
10	12947.0
11	13121.5
12	13296.0
13	13349.0
14	13402.0
15	13072.5
16	12743.0
17	12587.0
18	12431.0
19	12797.0
20	13163.0
21	14843.0
22	16523.0
23	17984.0
24	19445.0
25	22793.5
26	32575.5
27	39009.0
28	45476.0
29	51943.0
30	62208.5
31	72474.0
32	81747.0
33	91020.0
34	102359.5
35	113699.0
36	120039.5
37	126380.0
38	134590.5
39	142801.0
40	165874.5
41	188948.0
42	215387.5
43	241827.0
44	245697.5
45	249568.0
46	246038.5
47	242509.0
48	233673.5
49	224838.0
50	218533.5
51	212229.0
52	192199.0
53	172169.0
54	156236.5
55	140304.0
56	126472.0
57	112640.0
58	99932.0
59	87224.0
60	78143.5
61	69063.0
62	59264.5
63	49466.0
64	43072.5
65	36679.0
66	31163.0
67	25647.0
68	21645.5
69	17644.0
70	14346.5
71	11049.0
72	9293.0
73	7537.0
74	6044.5
75	3645.0
76	2738.0
77	2195.0
78	1652.0
79	1042.5
80	433.0
81	348.0
82	263.0
83	172.5
84	82.0
85	58.0
86	34.0
87	22.0
88	10.0
89	7.5
90	5.0
91	3.0
92	1.0
93	1.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2931986.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.148277526675447
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.72964058362261	14.106590893603176
2	7.551577475993766	2.740962475970523
3	2.68331372124741	1.460925663130027
4	1.4106827448542758	1.0240584782283073
5	0.8658418498581132	0.7856769092717547
6	0.6463125234224877	0.7037675426422337
7	0.4849798967486465	0.6161084832736987
8	0.37286781176638034	0.541352682296036
9	0.3457123703836538	0.5646675637914627
>10	4.751855748497605	22.106623611960313
>50	2.004148355924585	26.097862787436206
>100	1.1483014141413788	28.073433341869286
>500	0.004003022972859714	0.46086489575261536
>1k	5.718604246942448E-4	0.18255397499523732
>5k	0.0	0.0
>10k+	1.9062014156474825E-4	0.5345506957791182
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15452	0.5270147947500432	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.821314972172446E-5	0.0	0.0	0.02298783145622114	0.0
2	6.821314972172446E-5	0.0	0.0	0.07704675261068777	0.0
3	6.821314972172446E-5	0.0	0.0	0.13451633125124063	0.0
4	6.821314972172446E-5	0.0	0.0	0.24863693073568563	0.0
5	6.821314972172446E-5	0.0	0.0	0.43346045990669807	0.0
6	6.821314972172446E-5	0.0	0.0	0.777595800252798	0.0
7	6.821314972172446E-5	0.0	0.0	0.9410004004111888	0.0
8	6.821314972172446E-5	0.0	0.0	1.3891266875080577	0.0
9	6.821314972172446E-5	0.0	0.0	1.584864320634546	0.0
10	6.821314972172446E-5	0.0	0.0	1.8712572297412062	0.0
11	6.821314972172446E-5	0.0	0.0	2.1630389776758827	0.0
12	6.821314972172446E-5	0.0	0.0	2.403319797570657	0.0
13	6.821314972172446E-5	0.0	0.0	2.5178496759534323	0.0
14	6.821314972172446E-5	0.0	0.0	2.5768881570375846	0.0
15	6.821314972172446E-5	0.0	0.0	2.64117905065031	0.0
16	6.821314972172446E-5	0.0	0.0	2.7702383299238127	0.0
17	6.821314972172446E-5	0.0	0.0	2.9125991733930516	0.0
18	6.821314972172446E-5	0.0	0.0	3.089066591723153	0.0
19	6.821314972172446E-5	0.0	0.0	3.1994695745477637	0.0
20	6.821314972172446E-5	0.0	0.0	3.321980391447981	0.0
21	6.821314972172446E-5	0.0	0.0	3.4776086925380953	0.0
22	6.821314972172446E-5	0.0	0.0	3.6500515350346148	0.0
23	6.821314972172446E-5	0.0	0.0	3.8391724926380957	0.0
24	6.821314972172446E-5	0.0	0.0	3.981192270358726	0.0
25	6.821314972172446E-5	0.0	0.0	4.109126032661821	0.0
26	6.821314972172446E-5	0.0	0.0	4.229078856447472	0.0
27	6.821314972172446E-5	0.0	0.0	4.365266409866896	0.0
28	6.821314972172446E-5	0.0	0.0	4.502886439430474	0.0
29	6.821314972172446E-5	0.0	0.0	4.658855806269198	0.0
30	6.821314972172446E-5	0.0	0.0	4.868338389064614	0.0
31	6.821314972172446E-5	0.0	0.0	5.04265709317848	0.0
32	6.821314972172446E-5	0.0	0.0	5.211450532164887	0.0
33	6.821314972172446E-5	0.0	0.0	5.3728769509813485	0.0
34	6.821314972172446E-5	0.0	0.0	5.555210700187518	0.0
35	6.821314972172446E-5	0.0	0.0	5.763192593689056	0.0
36	6.821314972172446E-5	0.0	0.0	5.949209852980198	0.0
37	6.821314972172446E-5	0.0	0.0	6.138399023733401	0.0
38	6.821314972172446E-5	0.0	0.0	6.367970379121865	0.0
39	6.821314972172446E-5	0.0	0.0	6.756307840487642	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	9765	0.0	41.45161	1
TATCACG	60	3.6379788E-12	41.250004	1
TACGGGA	1245	0.0	40.120483	4
CGGGATC	760	0.0	39.967106	6
ACGGGTA	160	0.0	39.375	5
GTTAGCG	180	0.0	38.750004	1
ACGGGAT	1260	0.0	38.749996	5
ACACGAC	495	0.0	38.636364	26
AGGGCGA	2280	0.0	37.796055	6
TAAGGGA	3570	0.0	37.563026	4
TTAACGC	30	1.1403536E-4	37.500004	42
CGAGGGA	2040	0.0	37.5	4
AATGCGG	415	0.0	37.40964	2
TAGGGCG	905	0.0	37.292816	5
GTACGGG	965	0.0	37.07254	3
AACGGGA	1165	0.0	36.888412	4
TAACGGG	910	0.0	36.84066	3
ATAGGGA	4105	0.0	36.833126	4
GGGCGAT	4370	0.0	36.8135	7
AGGGATT	5390	0.0	36.56772	6
>>END_MODULE