FastQCFastQC Report
Thu 26 May 2016
SRR1547620_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547620_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1881686
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT259211.3775412050682208No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC20680.10990143945376646No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCATT207.034435E-445.018
CCGCGTA207.034435E-445.023
TATAGCG1200.044.9999961
CGTTTTT136850.043.783341
CGAATAT2400.042.18749614
TGTAGCG700.041.7857171
CGCTTAA650.041.5384623
GACGATT403.4597906E-739.3759
TCGACGG1150.039.1304322
CGGTCTA1350.038.33333631
ATAGGGC9400.038.058514
TACGGGA5800.038.0172424
TAGGGAT24600.037.7743955
CATACGA10550.037.53554518
GGGCGAT24050.037.422047
TCACGAC1450.037.24138325
CGGTTTT6300.037.1428571
ATAGGGA21900.037.089044
GTTTGCG1400.036.9642871
TACGTAG1400.036.9642871
Produced by FastQC (version 0.11.5)