FastQCFastQC Report
Thu 26 May 2016
SRR1547619_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547619_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2025000
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT212271.048246913580247No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT115600.042.294551
GCGATCG556.184564E-1140.9090929
GGGCGAT25100.039.7111557
CGGTCTA2100.039.64285731
TAGGGAC20700.039.2391325
TACGGGA6900.039.1304364
CGACGGT2200.038.86363628
TACGCGG1400.038.5714262
AGGGCGA13700.038.4306566
GGCGATA5400.038.3333328
CGTTAGG3250.038.076922
TAGGGAT31100.037.9823155
CGTAAGG2500.037.82
CTAGGGA14200.037.711274
AAGGGAT29050.037.6419945
ACGATAG301.14022885E-437.5000041
CGACTAA301.14022885E-437.50000441
ATACGAG900.037.51
AGGGATC18900.037.0238086
TCGTCCC9300.037.01612538
Produced by FastQC (version 0.11.5)