##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547619_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2025000
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.208143209876543	31.0	31.0	33.0	30.0	34.0
2	31.613866666666667	31.0	31.0	34.0	30.0	34.0
3	31.667220740740742	31.0	31.0	34.0	30.0	34.0
4	35.51281925925926	37.0	35.0	37.0	33.0	37.0
5	35.427751111111114	37.0	35.0	37.0	33.0	37.0
6	35.478476049382714	37.0	35.0	37.0	33.0	37.0
7	35.83572148148148	37.0	35.0	37.0	35.0	37.0
8	35.85761086419753	37.0	35.0	37.0	35.0	37.0
9	37.65899901234568	39.0	37.0	39.0	35.0	39.0
10	37.03274074074074	39.0	37.0	39.0	33.0	39.0
11	36.76593283950617	39.0	35.0	39.0	32.0	39.0
12	36.30611259259259	38.0	35.0	39.0	32.0	39.0
13	36.14184098765432	38.0	35.0	39.0	32.0	39.0
14	37.141953086419754	39.0	35.0	41.0	31.0	41.0
15	37.34474765432099	39.0	35.0	41.0	32.0	41.0
16	37.41086765432099	39.0	35.0	41.0	32.0	41.0
17	37.31433827160494	39.0	35.0	41.0	32.0	41.0
18	37.18006222222222	39.0	35.0	41.0	32.0	41.0
19	37.09312197530864	39.0	35.0	41.0	32.0	41.0
20	36.90439061728395	38.0	35.0	41.0	31.0	41.0
21	36.73251851851852	38.0	35.0	41.0	31.0	41.0
22	36.68840345679013	38.0	35.0	41.0	31.0	41.0
23	36.61642419753086	38.0	35.0	40.0	31.0	41.0
24	36.5145175308642	38.0	35.0	40.0	31.0	41.0
25	36.37943012345679	38.0	35.0	40.0	30.0	41.0
26	36.259460246913584	38.0	35.0	40.0	30.0	41.0
27	36.175054320987655	38.0	35.0	40.0	30.0	41.0
28	36.159555555555556	38.0	35.0	40.0	30.0	41.0
29	36.16507851851852	38.0	35.0	40.0	30.0	41.0
30	36.12422814814815	38.0	35.0	40.0	30.0	41.0
31	36.003329382716046	38.0	35.0	40.0	30.0	41.0
32	35.85168839506173	38.0	35.0	40.0	29.0	41.0
33	35.65003160493827	38.0	34.0	40.0	28.0	41.0
34	35.45691358024691	38.0	34.0	40.0	27.0	41.0
35	35.216394074074074	38.0	34.0	40.0	25.0	41.0
36	35.11445777777778	38.0	34.0	40.0	25.0	41.0
37	35.075253827160495	38.0	34.0	40.0	25.0	41.0
38	34.962522962962964	38.0	34.0	40.0	24.0	41.0
39	34.87735851851852	38.0	34.0	40.0	24.0	41.0
40	34.78425432098766	38.0	34.0	40.0	24.0	41.0
41	34.6731387654321	38.0	34.0	40.0	23.0	41.0
42	34.64169283950617	38.0	34.0	40.0	23.0	41.0
43	34.59028938271605	37.0	33.0	40.0	23.0	41.0
44	34.437906172839504	37.0	33.0	40.0	23.0	41.0
45	34.344236049382715	37.0	33.0	40.0	23.0	41.0
46	34.31086024691358	37.0	33.0	40.0	23.0	41.0
47	34.24763901234568	37.0	33.0	40.0	23.0	41.0
48	34.09163111111111	37.0	33.0	40.0	23.0	41.0
49	34.01634271604938	36.0	33.0	40.0	23.0	41.0
50	33.92320740740741	36.0	33.0	40.0	22.0	41.0
51	33.493912098765435	36.0	32.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	2.0
11	5.0
12	8.0
13	5.0
14	23.0
15	62.0
16	150.0
17	420.0
18	1022.0
19	2008.0
20	3495.0
21	5711.0
22	8580.0
23	12930.0
24	18971.0
25	27808.0
26	36680.0
27	41659.0
28	43802.0
29	47474.0
30	54223.0
31	64029.0
32	78279.0
33	99419.0
34	153791.0
35	207345.0
36	155948.0
37	193681.0
38	286872.0
39	480456.0
40	140.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.87713580246914	22.111703703703704	27.655506172839505	17.355654320987654
2	33.65046913580247	24.839555555555556	28.558123456790124	12.95185185185185
3	28.331012345679014	24.732888888888887	33.35575308641975	13.580345679012346
4	25.524691358024693	27.14464197530864	33.13802469135803	14.192641975308643
5	22.476493827160493	30.948395061728394	32.370172839506175	14.204938271604938
6	21.19041975308642	39.84325925925926	28.886469135802468	10.079851851851853
7	85.576	3.344	9.308148148148149	1.7718518518518518
8	85.6472098765432	2.8058765432098767	9.24079012345679	2.3061234567901234
9	80.4679012345679	5.124740740740741	11.233975308641975	3.1733827160493826
10	39.867703703703704	30.60627160493827	17.58804938271605	11.937975308641976
11	31.514074074074074	23.558074074074074	28.85032098765432	16.07753086419753
12	31.466024691358026	21.330617283950616	29.98014814814815	17.223209876543212
13	23.43683950617284	27.31827160493827	30.72854320987654	18.516345679012343
14	18.056	30.134172839506174	32.44730864197531	19.362518518518517
15	17.755259259259258	26.28775308641975	38.78113580246914	17.17585185185185
16	20.7479012345679	24.46385185185185	37.28276543209876	17.505481481481482
17	21.150962962962964	25.026172839506174	30.467209876543212	23.355654320987654
18	22.37916049382716	25.78967901234568	33.10681481481482	18.724345679012348
19	24.51837037037037	27.612592592592595	28.99708641975309	18.87195061728395
20	25.89595061728395	26.71639506172839	29.415703703703706	17.971950617283948
21	23.274666666666665	27.154913580246916	31.447407407407407	18.12301234567901
22	22.657283950617284	23.758814814814816	31.552641975308642	22.031259259259258
23	20.64320987654321	27.028049382716052	30.861234567901235	21.467506172839506
24	21.04888888888889	25.42987654320988	34.85911111111111	18.662123456790123
25	20.525975308641975	27.28977777777778	31.566271604938272	20.617975308641974
26	19.30069135802469	30.462123456790124	29.709382716049383	20.527802469135803
27	18.850716049382715	30.01916049382716	31.31516049382716	19.814962962962962
28	17.940345679012346	27.970320987654322	34.28148148148148	19.80785185185185
29	18.754765432098765	25.688296296296297	34.292	21.26493827160494
30	20.537037037037038	27.038716049382717	32.28493827160494	20.13930864197531
31	23.161086419753087	26.53051851851852	29.178962962962963	21.129432098765434
32	24.12706172839506	26.512493827160494	28.45293827160494	20.907506172839508
33	22.951802469135803	26.916543209876544	29.19649382716049	20.93516049382716
34	19.827012345679012	26.812493827160495	30.628345679012348	22.73214814814815
35	20.476395061728393	26.718666666666667	31.39856790123457	21.40637037037037
36	23.233481481481483	27.24330864197531	29.616641975308646	19.906567901234567
37	20.728444444444445	29.659506172839507	29.81076543209877	19.801283950617286
38	20.409777777777776	28.822074074074074	29.922024691358022	20.84612345679012
39	20.369827160493827	29.441728395061727	29.12785185185185	21.06059259259259
40	22.844444444444445	26.824049382716048	30.15906172839506	20.172444444444444
41	19.028444444444446	26.57333333333333	30.95259259259259	23.44562962962963
42	21.641975308641975	26.671308641975312	28.719555555555555	22.96716049382716
43	21.22098765432099	27.102666666666668	29.2679012345679	22.408444444444445
44	20.325333333333333	28.99545679012346	28.93738271604938	21.741827160493827
45	19.515654320987654	30.310765432098762	27.8641975308642	22.309382716049384
46	21.119654320987653	29.53940740740741	28.273827160493823	21.06711111111111
47	20.570617283950618	27.71762962962963	29.893185185185185	21.818567901234566
48	20.65441975308642	26.910271604938274	30.918913580246915	21.516395061728392
49	20.52874074074074	26.313135802469134	30.57372839506173	22.584395061728397
50	19.541382716049384	28.080246913580247	31.06888888888889	21.30948148148148
51	19.258320987654322	29.26681481481481	29.01762962962963	22.457234567901235
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1402.0
1	2010.5
2	2619.0
3	17901.0
4	33183.0
5	23160.5
6	13138.0
7	12893.0
8	12648.0
9	13267.0
10	13886.0
11	14394.5
12	14903.0
13	14600.0
14	14297.0
15	13620.5
16	12944.0
17	12417.5
18	11891.0
19	11534.0
20	11177.0
21	11738.5
22	12300.0
23	12677.0
24	13054.0
25	14910.0
26	19415.5
27	22065.0
28	26327.5
29	30590.0
30	34517.5
31	38445.0
32	45069.0
33	51693.0
34	58661.0
35	65629.0
36	69480.0
37	73331.0
38	82151.0
39	90971.0
40	103849.5
41	116728.0
42	131020.0
43	145312.0
44	149377.5
45	153443.0
46	159499.0
47	165555.0
48	166669.5
49	167784.0
50	160739.0
51	153694.0
52	140170.0
53	126646.0
54	112237.0
55	97828.0
56	89230.0
57	80632.0
58	73935.0
59	67238.0
60	61123.5
61	55009.0
62	49237.0
63	43465.0
64	37470.5
65	31476.0
66	26885.0
67	22294.0
68	18616.0
69	14938.0
70	12731.0
71	10524.0
72	8478.5
73	6433.0
74	5258.5
75	3266.0
76	2448.0
77	1905.5
78	1363.0
79	1000.0
80	637.0
81	466.5
82	296.0
83	229.5
84	163.0
85	101.5
86	40.0
87	29.0
88	18.0
89	16.0
90	14.0
91	8.0
92	2.0
93	2.0
94	2.0
95	1.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2025000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.15213871021779
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.54174490377093	17.95257233844428
2	7.089225313788866	3.282614556256542
3	2.644636054973641	1.8368694234837877
4	1.4652197787667796	1.3569188623585244
5	0.9981443318201203	1.155458801155854
6	0.7295253682600088	1.0134043511147066
7	0.542715920061962	0.8795523983062612
8	0.4655831953874846	0.8623397376605982
9	0.3851202006206005	0.8024720674387543
>10	6.167099851249292	35.80881215610729
>50	1.805194268687296	28.38292865485461
>100	0.16189494799294202	4.8531851509372785
>500	0.002597243080082663	0.43304869034385896
>1k	0.0010821846167011096	0.31614258127509903
>5k	0.0	0.0
>10k+	2.164369233402219E-4	1.0636802302625659
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21227	1.048246913580247	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.876543209876543E-5	0.0	0.0	0.03590123456790124	0.0
2	9.876543209876543E-5	0.0	0.0	0.11871604938271604	0.0
3	9.876543209876543E-5	0.0	0.0	0.2386172839506173	0.0
4	9.876543209876543E-5	0.0	0.0	0.4143703703703704	0.0
5	9.876543209876543E-5	0.0	0.0	0.7522962962962964	0.0
6	9.876543209876543E-5	0.0	0.0	1.4408888888888889	0.0
7	9.876543209876543E-5	0.0	0.0	1.8019753086419754	0.0
8	9.876543209876543E-5	0.0	0.0	2.6166913580246915	0.0
9	9.876543209876543E-5	0.0	0.0	3.071901234567901	0.0
10	9.876543209876543E-5	0.0	0.0	3.6410864197530866	0.0
11	9.876543209876543E-5	0.0	0.0	4.122222222222222	0.0
12	9.876543209876543E-5	0.0	0.0	4.469679012345679	0.0
13	9.876543209876543E-5	0.0	0.0	4.656691358024691	0.0
14	9.876543209876543E-5	0.0	0.0	4.7803456790123455	0.0
15	9.876543209876543E-5	0.0	0.0	4.870074074074074	0.0
16	9.876543209876543E-5	0.0	0.0	5.026123456790123	0.0
17	9.876543209876543E-5	0.0	0.0	5.19516049382716	0.0
18	9.876543209876543E-5	0.0	0.0	5.407753086419753	0.0
19	9.876543209876543E-5	0.0	0.0	5.540592592592593	0.0
20	1.4814814814814815E-4	0.0	0.0	5.686024691358025	0.0
21	1.4814814814814815E-4	0.0	0.0	5.871604938271605	0.0
22	1.4814814814814815E-4	0.0	0.0	6.070913580246914	0.0
23	1.4814814814814815E-4	0.0	0.0	6.27446913580247	0.0
24	1.4814814814814815E-4	0.0	0.0	6.440740740740741	0.0
25	1.4814814814814815E-4	0.0	0.0	6.586716049382716	0.0
26	1.4814814814814815E-4	0.0	0.0	6.72879012345679	0.0
27	1.4814814814814815E-4	0.0	0.0	6.876987654320987	0.0
28	1.4814814814814815E-4	0.0	0.0	7.034172839506173	0.0
29	1.4814814814814815E-4	0.0	0.0	7.214172839506173	0.0
30	1.4814814814814815E-4	0.0	0.0	7.4595061728395065	0.0
31	1.4814814814814815E-4	0.0	0.0	7.656246913580247	0.0
32	1.4814814814814815E-4	0.0	0.0	7.843259259259259	0.0
33	1.4814814814814815E-4	0.0	0.0	8.026765432098765	0.0
34	1.4814814814814815E-4	0.0	0.0	8.218666666666667	0.0
35	1.4814814814814815E-4	0.0	0.0	8.434814814814814	0.0
36	1.4814814814814815E-4	0.0	0.0	8.621728395061728	0.0
37	1.4814814814814815E-4	0.0	0.0	8.821185185185184	0.0
38	1.4814814814814815E-4	0.0	0.0	9.050666666666666	0.0
39	1.4814814814814815E-4	0.0	0.0	9.41604938271605	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	11560	0.0	42.29455	1
GCGATCG	55	6.184564E-11	40.909092	9
GGGCGAT	2510	0.0	39.711155	7
CGGTCTA	210	0.0	39.642857	31
TAGGGAC	2070	0.0	39.239132	5
TACGGGA	690	0.0	39.130436	4
CGACGGT	220	0.0	38.863636	28
TACGCGG	140	0.0	38.571426	2
AGGGCGA	1370	0.0	38.430656	6
GGCGATA	540	0.0	38.333332	8
CGTTAGG	325	0.0	38.07692	2
TAGGGAT	3110	0.0	37.982315	5
CGTAAGG	250	0.0	37.8	2
CTAGGGA	1420	0.0	37.71127	4
AAGGGAT	2905	0.0	37.641994	5
ACGATAG	30	1.14022885E-4	37.500004	1
CGACTAA	30	1.14022885E-4	37.500004	41
ATACGAG	90	0.0	37.5	1
AGGGATC	1890	0.0	37.023808	6
TCGTCCC	930	0.0	37.016125	38
>>END_MODULE