FastQCFastQC Report
Thu 26 May 2016
SRR1547617_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547617_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2546410
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT593642.3312820794765967No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTT53040.2082932442144038No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCT38730.15209648092805167No Hit
CGTTCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTC34440.1352492332342396Illumina PCR Primer Index 11 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCTG25740.10108348616287242Illumina PCR Primer Index 11 (95% over 23bp)
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25510.1001802537690317No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACCC207.0349034E-445.00000420
CAGCGCG253.8921462E-545.01
ACCGCGA253.8921462E-545.010
CGTTTTT291500.044.0351641
TCTAGCG1100.042.9545441
CGAACGG1050.042.8571472
TACGGGA12250.040.9591874
TCGACGG556.184564E-1140.9090922
CGACGGT1550.040.6451628
AGGGCGA18450.040.365856
TACGGGT950.040.2631574
GCGCGAC3750.040.1999979
TAGCGGA451.9299478E-840.0000043
TATTGCG1750.039.8571431
CTATGCG1300.039.8076931
ACGGGAT13850.039.801445
GCGATCG2100.039.642869
CGGTCTA1650.039.54545231
GGCGATC7250.039.4137958
ACGCTAA403.4604273E-739.37500425
Produced by FastQC (version 0.11.5)