##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547617_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2546410
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.280317388009	31.0	31.0	33.0	30.0	34.0
2	31.673036156785436	31.0	31.0	34.0	30.0	34.0
3	31.51135323848084	31.0	31.0	34.0	30.0	34.0
4	35.46528956452417	37.0	35.0	37.0	33.0	37.0
5	35.44028966270161	37.0	35.0	37.0	33.0	37.0
6	35.50738058678689	37.0	35.0	37.0	33.0	37.0
7	35.93682439198715	37.0	35.0	37.0	35.0	37.0
8	35.988261120557965	37.0	35.0	37.0	35.0	37.0
9	37.792037810093426	39.0	38.0	39.0	35.0	39.0
10	37.18867425120071	39.0	37.0	39.0	33.0	39.0
11	36.86719577758491	39.0	37.0	39.0	32.0	39.0
12	36.59285503905498	39.0	35.0	39.0	32.0	39.0
13	36.51036753704235	39.0	35.0	39.0	32.0	39.0
14	37.62888890634265	40.0	36.0	41.0	32.0	41.0
15	37.787319009900216	40.0	36.0	41.0	33.0	41.0
16	37.815168413570476	40.0	36.0	41.0	33.0	41.0
17	37.6879932925177	40.0	36.0	41.0	32.0	41.0
18	37.46287675590341	39.0	36.0	41.0	32.0	41.0
19	37.278313390223886	39.0	36.0	41.0	32.0	41.0
20	36.99836868375478	38.0	35.0	41.0	32.0	41.0
21	36.837594888490074	38.0	35.0	41.0	31.0	41.0
22	36.80144438641067	38.0	35.0	41.0	31.0	41.0
23	36.70653743898272	38.0	35.0	40.0	31.0	41.0
24	36.6186839511312	38.0	35.0	40.0	31.0	41.0
25	36.52343141913517	38.0	35.0	40.0	31.0	41.0
26	36.41655860603752	38.0	35.0	40.0	31.0	41.0
27	36.344949163724614	38.0	35.0	40.0	31.0	41.0
28	36.277328866914594	38.0	35.0	40.0	31.0	41.0
29	36.22935937260692	38.0	35.0	40.0	30.0	41.0
30	36.106806445152195	38.0	35.0	40.0	30.0	41.0
31	35.888446479553565	38.0	35.0	40.0	30.0	41.0
32	35.57639932296841	38.0	35.0	40.0	27.0	41.0
33	35.20965201990253	38.0	34.0	40.0	25.0	41.0
34	34.87014581312514	38.0	34.0	40.0	23.0	41.0
35	34.52442536747814	38.0	34.0	40.0	21.0	41.0
36	34.37910744931099	38.0	34.0	40.0	20.0	41.0
37	34.32735341127312	38.0	33.0	40.0	20.0	41.0
38	34.1999956801929	38.0	33.0	40.0	18.0	41.0
39	34.11542249676996	38.0	33.0	40.0	18.0	41.0
40	34.04414567960384	38.0	33.0	40.0	18.0	41.0
41	33.90498309384584	38.0	33.0	40.0	18.0	41.0
42	33.85220329797637	38.0	33.0	40.0	18.0	41.0
43	33.780240416900654	37.0	33.0	40.0	18.0	41.0
44	33.64229287506725	37.0	33.0	40.0	17.0	41.0
45	33.559164078054984	37.0	33.0	40.0	17.0	41.0
46	33.51964216288815	37.0	33.0	40.0	17.0	41.0
47	33.46364057634081	37.0	33.0	40.0	17.0	41.0
48	33.29844722570207	37.0	32.0	40.0	15.0	41.0
49	33.219802388460614	36.0	32.0	40.0	15.0	41.0
50	33.15116850782081	36.0	32.0	40.0	15.0	41.0
51	32.79345038701545	36.0	31.0	40.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	4.0
12	6.0
13	7.0
14	14.0
15	65.0
16	181.0
17	550.0
18	1385.0
19	2787.0
20	4840.0
21	7755.0
22	12228.0
23	18730.0
24	29377.0
25	46766.0
26	64051.0
27	68862.0
28	65291.0
29	63733.0
30	67647.0
31	76314.0
32	90704.0
33	112360.0
34	165373.0
35	239925.0
36	196308.0
37	237317.0
38	345352.0
39	628297.0
40	180.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.189883011769513	22.253250654843484	27.479156930737787	22.07770940264922
2	37.51803519464658	23.705805428033976	27.07859299955624	11.697566377763204
3	25.888761040052465	23.52987146610326	38.458182303713855	12.12318519013042
4	22.209974041886422	26.35329738730212	38.40728712186961	13.029441448941844
5	20.81031727019608	29.85681803008942	36.97244355779313	12.360421141921371
6	19.456803892538908	37.27164910599628	33.362577118374496	9.908969883090311
7	81.53294245624232	2.413044246606006	14.550956051853396	1.5030572452982827
8	82.3002187393232	1.6810725688321992	14.20195490906806	1.8167537827765363
9	77.2198506917582	4.859115382047667	15.754611394080293	2.1664225321138386
10	34.032579199736105	37.56480692425807	19.97922565494166	8.423388221064165
11	23.219905671121303	23.10570567976092	38.13136926103809	15.543019388079687
12	24.50371307055816	22.539771678559227	36.443699168633486	16.512816082249127
13	19.656575335472294	26.470207075844034	37.63184247627051	16.241375112413163
14	14.846273773665668	30.08639614201955	37.722676238311976	17.344653846002803
15	14.490557294387	27.369237475504733	43.09039000003927	15.049815230069
16	18.0944938167852	25.45057551611877	41.57747574035603	14.87745492674
17	17.812253329196793	25.901759732329044	36.30075282456478	19.985234113909385
18	18.805573336579734	26.57411807210936	37.741487034688056	16.878821556622853
19	20.442230434219155	28.772585718717725	34.2375736821643	16.547610164898817
20	22.422037299570768	27.590254515180195	34.144658558519644	15.843049626729396
21	20.456603610573318	27.419465050797005	35.91314831468617	16.210783023943513
22	19.693607863619764	24.35342305441779	36.336960662265696	19.616008419696747
23	16.384792708165612	28.82033922267035	36.0058670834626	18.78900098570144
24	16.94424699871584	27.498085540034793	39.4382287220047	16.119438739244664
25	17.98139341268688	28.949815622778736	35.89394480857364	17.174846155960743
26	16.55801697291481	32.21024893870194	33.98726049614948	17.24447359223377
27	16.57023024571848	30.348372807207006	36.3357432620827	16.74565368499181
28	16.148891969478598	29.028671737858396	38.82960717245062	15.992829120212376
29	16.353140303407542	26.79678449267793	38.414041729336596	18.436033474577933
30	18.014184675680664	28.22805439815269	36.89323400395066	16.864526922215983
31	20.428446322469675	28.26834641711272	34.30696549259546	16.99624176782215
32	20.905863549074972	28.82328454569374	33.755326125800636	16.515525779430646
33	19.707391975369244	29.5610290565934	33.331003255563715	17.40057571247364
34	16.94679961200278	30.202481140114905	33.975439933082264	18.875279314800053
35	17.655837041167764	30.410774384329308	33.126244398977384	18.807144175525544
36	20.411402719907635	29.938108945535085	31.577829179118837	18.07265915543844
37	17.90285146539638	31.980513742877225	32.22183387592729	17.894800915799106
38	18.391146751701413	31.579321476117357	31.113999709394797	18.915532062786433
39	17.7699977615545	31.373345219348025	31.082347304636727	19.77430971446075
40	20.018221731771398	29.846450493047072	31.341064479011628	18.794263296169902
41	17.36393589406262	29.517948798504563	32.52512360538955	20.59299170204327
42	19.318255897518466	30.384423561013346	30.08631760007226	20.211002941395925
43	19.19176409140712	30.297359812441833	31.1127037672645	19.398172328886552
44	18.896485640568486	31.629234883620473	30.66183371884339	18.81244575696765
45	18.096692991309332	32.63311878291398	29.576933800919726	19.693254424856953
46	19.466071842319185	31.765190994380323	29.703779045793883	19.06495811750661
47	18.888120923182047	30.691247678103682	31.054621997243174	19.36600940147109
48	19.226440361135875	29.417807815709175	31.955615945586143	19.400135877568815
49	18.499534638962302	29.21505177877875	31.82134848669303	20.464065095565914
50	17.35698493172741	30.65920256360916	32.781248895503865	19.20256360915956
51	16.71011345384286	31.622990798810875	31.325552444421756	20.341343302924507
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1565.0
1	2410.0
2	3255.0
3	44485.5
4	85716.0
5	58532.5
6	31349.0
7	30524.0
8	29699.0
9	31915.0
10	34131.0
11	35970.5
12	37810.0
13	37958.5
14	38107.0
15	37105.5
16	36104.0
17	34413.0
18	32722.0
19	30263.5
20	27805.0
21	26965.0
22	26125.0
23	26084.5
24	26044.0
25	28006.5
26	34218.5
27	38468.0
28	45142.0
29	51816.0
30	58093.5
31	64371.0
32	72592.5
33	80814.0
34	89722.0
35	98630.0
36	107270.0
37	115910.0
38	122148.0
39	128386.0
40	144723.5
41	161061.0
42	169615.5
43	178170.0
44	186661.5
45	195153.0
46	200172.0
47	205191.0
48	202792.0
49	200393.0
50	180508.5
51	160624.0
52	140973.0
53	121322.0
54	105610.0
55	89898.0
56	78016.0
57	66134.0
58	57707.5
59	49281.0
60	43698.5
61	38116.0
62	31283.0
63	24450.0
64	19903.5
65	15357.0
66	12253.5
67	9150.0
68	6889.0
69	4628.0
70	4117.5
71	3607.0
72	3002.0
73	2397.0
74	1952.5
75	1002.0
76	496.0
77	433.5
78	371.0
79	251.0
80	131.0
81	97.0
82	63.0
83	61.5
84	60.0
85	44.5
86	29.0
87	19.5
88	10.0
89	10.5
90	11.0
91	6.0
92	1.0
93	1.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2546410.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.967730439871428
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.72712968152776	19.664616905550997
2	7.645938444571092	3.970953369769439
3	3.4478347845004684	2.6859733285536107
4	2.0923448662330983	2.1733378989435974
5	1.3758585317637744	1.7863961738119483
6	1.0001451971894302	1.5582900528808308
7	0.7179045200833636	1.3049645741363036
8	0.5990485122121024	1.2444744228423925
9	0.48428872904580417	1.1318291253836439
>10	5.601711659051654	33.51664370680128
>50	1.0735144528195204	19.13324035591857
>100	0.22967233620876604	7.985067212469042
>500	0.0027649708759019008	0.4791454559859684
>1k	0.0015360949310566116	0.7855320899710021
>5k	1.5360949310566115E-4	0.21157072082497308
>10k+	1.5360949310566115E-4	2.3679646061564292
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	59364	2.3312820794765967	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTT	5304	0.2082932442144038	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCT	3873	0.15209648092805167	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTC	3444	0.1352492332342396	Illumina PCR Primer Index 11 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCTG	2574	0.10108348616287242	Illumina PCR Primer Index 11 (95% over 23bp)
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2551	0.1001802537690317	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.050149033344983725	0.0
2	0.0	0.0	0.0	0.13450308473497982	0.0
3	0.0	0.0	0.0	0.2970849156263131	0.0
4	0.0	0.0	0.0	0.47270470976786927	0.0
5	0.0	0.0	0.0	0.8614480778821949	0.0
6	0.0	0.0	0.0	1.7378191257495847	0.0
7	0.0	0.0	0.0	2.302064475084531	0.0
8	0.0	0.0	0.0	3.2388735513919595	0.0
9	0.0	0.0	0.0	3.7441338983117407	0.0
10	0.0	0.0	0.0	4.22304342191556	0.0
11	0.0	0.0	0.0	4.5967460071237545	0.0
12	0.0	0.0	0.0	4.885976728021018	0.0
13	0.0	0.0	0.0	5.069372174944333	0.0
14	0.0	0.0	0.0	5.190798025455445	0.0
15	0.0	0.0	0.0	5.284773465388527	0.0
16	0.0	0.0	0.0	5.415585078600854	0.0
17	0.0	0.0	0.0	5.580837335700064	0.0
18	0.0	0.0	0.0	5.782650869263001	0.0
19	0.0	0.0	0.0	5.924968877753386	0.0
20	0.0	0.0	0.0	6.0620245757753075	0.0
21	0.0	0.0	0.0	6.2263736004806765	0.0
22	0.0	0.0	0.0	6.412910725295612	0.0
23	0.0	0.0	0.0	6.610600806625799	0.0
24	0.0	0.0	0.0	6.763953958710498	0.0
25	0.0	0.0	0.0	6.904936754096944	0.0
26	0.0	0.0	0.0	7.034805863941784	0.0
27	0.0	0.0	0.0	7.174374904277002	0.0
28	0.0	0.0	0.0	7.33220494735726	0.0
29	0.0	0.0	0.0	7.500402527479864	0.0
30	0.0	0.0	0.0	7.70834233293146	0.0
31	0.0	0.0	0.0	7.891894863749357	0.0
32	0.0	0.0	0.0	8.077646569091387	0.0
33	0.0	0.0	0.0	8.251342085524326	0.0
34	0.0	0.0	0.0	8.437997023260198	0.0
35	0.0	0.0	0.0	8.641302853821655	0.0
36	0.0	0.0	0.0	8.833966250525249	0.0
37	3.9270973645249584E-5	0.0	0.0	9.037468435954933	0.0
38	3.9270973645249584E-5	0.0	0.0	9.247293248141501	0.0
39	3.9270973645249584E-5	0.0	0.0	9.517752443636335	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTACCC	20	7.0349034E-4	45.000004	20
CAGCGCG	25	3.8921462E-5	45.0	1
ACCGCGA	25	3.8921462E-5	45.0	10
CGTTTTT	29150	0.0	44.035164	1
TCTAGCG	110	0.0	42.954544	1
CGAACGG	105	0.0	42.857147	2
TACGGGA	1225	0.0	40.959187	4
TCGACGG	55	6.184564E-11	40.909092	2
CGACGGT	155	0.0	40.64516	28
AGGGCGA	1845	0.0	40.36585	6
TACGGGT	95	0.0	40.263157	4
GCGCGAC	375	0.0	40.199997	9
TAGCGGA	45	1.9299478E-8	40.000004	3
TATTGCG	175	0.0	39.857143	1
CTATGCG	130	0.0	39.807693	1
ACGGGAT	1385	0.0	39.80144	5
GCGATCG	210	0.0	39.64286	9
CGGTCTA	165	0.0	39.545452	31
GGCGATC	725	0.0	39.413795	8
ACGCTAA	40	3.4604273E-7	39.375004	25
>>END_MODULE