FastQCFastQC Report
Thu 26 May 2016
SRR1547616_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547616_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2466759
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT289571.1738884909308125No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT175300.043.2929271
GCGTAAG2650.041.6037751
AATCGCG603.6379788E-1241.2499961
TACGGGT1750.041.1428574
TCGATAG1250.039.6000021
CGTAAGG4050.039.4444472
CGAGGGA16550.039.4259834
CGTTAGG3250.038.769232
AGGGCGA21300.038.661976
AGCGCGA356.2512026E-638.57142626
GACCGAT12850.037.996119
ACACGCG2450.037.6530636
TACGGGA9000.037.5000044
TTAGCGG5400.037.52
ACGGGTA2400.037.4999965
AAGGGAT42850.037.491255
GAGGGAT38600.037.4805685
GGGCGAT38800.037.461347
GCGATAT2050.037.3170749
TTAGGGA33000.037.1590924
Produced by FastQC (version 0.11.5)