##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1547616_1.fastq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 2466759 Sequences flagged as poor quality 0 Sequence length 51 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.351738455195665 31.0 31.0 33.0 30.0 34.0 2 31.753830025551746 31.0 31.0 34.0 30.0 34.0 3 31.753272613984585 31.0 31.0 34.0 30.0 34.0 4 35.615552228653065 37.0 35.0 37.0 33.0 37.0 5 35.56572895852412 37.0 35.0 37.0 33.0 37.0 6 35.62618926291543 37.0 35.0 37.0 33.0 37.0 7 35.969864101032975 37.0 35.0 37.0 35.0 37.0 8 36.014994168461534 37.0 35.0 37.0 35.0 37.0 9 37.855971742679365 39.0 38.0 39.0 35.0 39.0 10 37.24918729393508 39.0 37.0 39.0 34.0 39.0 11 36.93187133400547 39.0 37.0 39.0 33.0 39.0 12 36.557867225780875 39.0 35.0 39.0 32.0 39.0 13 36.44843010606225 39.0 35.0 39.0 32.0 39.0 14 37.53003556488493 40.0 36.0 41.0 32.0 41.0 15 37.702778828414125 40.0 36.0 41.0 33.0 41.0 16 37.748890750981346 40.0 36.0 41.0 33.0 41.0 17 37.633989376343614 40.0 35.0 41.0 33.0 41.0 18 37.47985109206047 39.0 36.0 41.0 32.0 41.0 19 37.37475448554155 39.0 36.0 41.0 32.0 41.0 20 37.16406588564185 39.0 35.0 41.0 32.0 41.0 21 37.01326720607891 39.0 35.0 41.0 32.0 41.0 22 36.970850010073946 38.0 35.0 41.0 32.0 41.0 23 36.89128933957472 38.0 35.0 41.0 32.0 41.0 24 36.79333368196893 38.0 35.0 40.0 32.0 41.0 25 36.70182089129907 38.0 35.0 40.0 31.0 41.0 26 36.60622460483574 38.0 35.0 40.0 31.0 41.0 27 36.5292523509593 38.0 35.0 40.0 31.0 41.0 28 36.52497467324534 38.0 35.0 40.0 31.0 41.0 29 36.53544347056198 38.0 35.0 40.0 31.0 41.0 30 36.508113277381376 38.0 35.0 40.0 31.0 41.0 31 36.385358277805004 38.0 35.0 40.0 30.0 41.0 32 36.1703940271425 38.0 35.0 41.0 30.0 41.0 33 35.93114933400466 38.0 35.0 41.0 29.0 41.0 34 35.696492036717004 38.0 35.0 41.0 28.0 41.0 35 35.426960234056104 38.0 35.0 41.0 26.0 41.0 36 35.27592034730592 38.0 35.0 40.0 25.0 41.0 37 35.2461375432298 38.0 34.0 40.0 25.0 41.0 38 35.11043154195444 38.0 34.0 40.0 24.0 41.0 39 35.02936525213853 38.0 34.0 40.0 24.0 41.0 40 34.96622450754208 38.0 34.0 40.0 24.0 41.0 41 34.86486235582803 38.0 34.0 40.0 23.0 41.0 42 34.81817802225511 38.0 34.0 40.0 23.0 41.0 43 34.77311119570254 38.0 34.0 40.0 23.0 41.0 44 34.638092330868155 38.0 34.0 40.0 23.0 41.0 45 34.56353944588831 38.0 34.0 40.0 23.0 41.0 46 34.53494808370011 38.0 34.0 40.0 23.0 41.0 47 34.48391107522056 37.0 34.0 40.0 23.0 41.0 48 34.32429272579932 37.0 33.0 40.0 23.0 41.0 49 34.25817155222703 37.0 33.0 40.0 23.0 41.0 50 34.176355290484395 37.0 33.0 40.0 22.0 41.0 51 33.765069064306644 36.0 33.0 40.0 21.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 0.0 11 3.0 12 3.0 13 9.0 14 15.0 15 45.0 16 119.0 17 331.0 18 761.0 19 1660.0 20 2908.0 21 4926.0 22 8238.0 23 13064.0 24 20970.0 25 33277.0 26 45442.0 27 50832.0 28 50839.0 29 52243.0 30 58294.0 31 70216.0 32 86111.0 33 110612.0 34 176401.0 35 258783.0 36 183221.0 37 232333.0 38 359332.0 39 645584.0 40 186.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 30.61048931006231 23.146728156256856 28.43901653951602 17.803765994164813 2 33.90164989769978 24.955538826452038 28.911052924100005 12.231758351748184 3 27.014515807989348 24.65749592886861 35.21912760833142 13.108860654810625 4 23.869620015575094 27.208778806523053 34.9600427119147 13.961558465987151 5 21.7167951956393 31.58974995125182 33.48478712350902 13.208667729599851 6 20.420357237979065 39.46044181859679 29.94743304878993 10.171767894634215 7 85.8303952676366 2.7696260558895296 10.025989567687803 1.3739891087860632 8 86.75841458367032 2.0860975879686663 9.427349814067771 1.7281380142932488 9 81.99970082200977 4.511344642909989 11.256186761657705 2.2327677734225353 10 39.90077668714292 32.969130750105705 16.77695307891853 10.353139483832836 11 27.921130519844056 24.41523472702441 31.4271479297329 16.23648682339864 12 28.67004843197086 22.03648593154013 31.436958373314944 17.856507263174066 13 21.452764538408495 28.908296270531498 32.14845876715155 17.490480423908455 14 16.333456166573225 31.865982854425585 32.824446976782085 18.976114002219106 15 15.98530703647985 26.891520412006198 40.72724575039556 16.395926801118392 16 19.633859651469805 24.894568135760323 38.88203103748684 16.589541175283035 17 19.087596315651428 25.024049775434083 32.46048762769286 23.427866281221636 18 20.955837193661804 25.774832482621935 34.98773086466899 18.281599459047275 19 23.75169199747523 27.805594304105103 30.270691218720597 18.17202247969907 20 25.133180825528555 26.519210024165314 31.277802168756658 17.069806981549473 21 22.07503854247618 26.977665836022087 32.91014647154424 18.037149149957497 22 22.231154320304498 24.01410109378338 32.45659588147849 21.298148704433633 23 19.10827121741524 28.302197336667263 31.607424965308734 20.982106480608767 24 18.916440560265517 26.27265168587608 36.84733693076624 17.963570823092162 25 18.916197326127115 28.56148492819931 33.51235365919411 19.009964086479467 26 18.417648420457773 31.445795880343397 30.88027650856853 19.256279190630295 27 17.57411242849423 30.612678417307894 33.227323788014964 18.585885366182914 28 17.189883567871853 28.456407780411464 36.29799262919482 18.05571602252186 29 17.797360828520336 25.772805531468617 36.080784543605596 20.349049096405444 30 18.791701986290512 28.17214815067058 34.73359172906636 18.302558133972553 31 23.324937701656303 27.411554999900677 30.58251738414657 18.68098991429645 32 23.379381609634343 27.207805869969466 30.990177800101264 18.42263472029493 33 22.95003281633917 28.204619908146682 29.89546202121894 18.94988525429521 34 18.972789802327668 28.10002922863563 31.66701732921619 21.260163639820508 35 19.967374194236243 27.76083922263991 32.16962013719216 20.102166445931687 36 22.599735118023283 27.299018671868637 31.18707583513428 18.9141703749738 37 20.071600022539695 29.61533737183081 32.16325551057076 18.14980709505874 38 19.460879640045906 29.705293464014925 30.881654835352784 19.95217206058638 39 19.471987332366073 29.58173052170885 31.48390256202572 19.46237958389936 40 21.89508581908488 27.725610811595296 30.7513624152177 19.627940954102126 41 18.178022255112882 27.444472686630515 32.15518824498056 22.222316813276045 42 20.562122201641913 27.87276746532596 30.05328043801604 21.511829895016092 43 20.68305010744868 28.187350284320438 29.97706707465139 21.152532533579485 44 19.444015406450326 30.29169043266894 29.839518169387443 20.42477599149329 45 18.574574978747417 31.879766122268126 28.53517510222928 21.010483796755175 46 21.161734891815538 29.979783189196834 29.020265052240614 19.838216866747015 47 19.900525345199917 29.3083758891728 30.27425865275043 20.516840112876856 48 20.565243706418016 27.390880098136865 31.769297284412463 20.274578911032652 49 19.935267287967733 27.062149160092254 31.13834792940859 21.864235622531428 50 19.220483233262755 29.290011711723764 31.693367694209286 19.7961373608042 51 18.42360765684852 30.0426186749496 29.893799921273217 21.63997374692866 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1387.0 1 1986.5 2 2586.0 3 23945.5 4 45305.0 5 32179.5 6 19054.0 7 18697.5 8 18341.0 9 18974.0 10 19607.0 11 20030.0 12 20453.0 13 20079.0 14 19705.0 15 18887.5 16 18070.0 17 17458.5 18 16847.0 19 16052.0 20 15257.0 21 15224.0 22 15191.0 23 16483.5 24 17776.0 25 19663.5 26 25154.0 27 28757.0 28 35098.5 29 41440.0 30 48288.5 31 55137.0 32 63133.5 33 71130.0 34 81324.5 35 91519.0 36 98076.5 37 104634.0 38 115355.5 39 126077.0 40 145025.0 41 163973.0 42 180428.0 43 196883.0 44 203370.0 45 209857.0 46 214724.5 47 219592.0 48 213957.0 49 208322.0 50 196745.5 51 185169.0 52 163356.5 53 141544.0 54 123870.0 55 106196.0 56 94224.5 57 82253.0 58 71386.5 59 60520.0 60 52094.0 61 43668.0 62 37417.5 63 31167.0 64 25754.5 65 20342.0 66 15819.5 67 11297.0 68 8668.0 69 6039.0 70 5230.0 71 4421.0 72 3407.5 73 2394.0 74 1881.5 75 1150.0 76 931.0 77 726.5 78 522.0 79 405.5 80 289.0 81 196.5 82 104.0 83 81.5 84 59.0 85 35.0 86 11.0 87 8.5 88 6.0 89 5.0 90 4.0 91 2.5 92 1.0 93 1.5 94 2.0 95 1.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 2466759.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 22.47773833657103 #Duplication Level Percentage of deduplicated Percentage of total 1 77.24034197953382 17.36188196043224 2 7.635347137942515 3.4325067015111674 3 2.9185000958525698 1.9680384446949462 4 1.6173605829152986 1.454184319146163 5 1.0621278752782914 1.1937116230241793 6 0.7694999674631511 1.0377971351181978 7 0.6044198014614357 0.9510193099884656 8 0.47780157318581884 0.8591918991098265 9 0.38920531555218707 0.7873609717966159 >10 5.027425094677716 27.505765360829937 >50 1.788627790007652 28.30065466606511 >100 0.465678948412698 13.160996053230456 >500 0.0018319188583424896 0.2743237905115084 >1k 0.0016487269725082407 0.520194002631625 >5k 0.0 0.0 >10k+ 1.8319188583424897E-4 1.192373761909599 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 28957 1.1738884909308125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 4.0539023066298735E-5 0.0 0.0 0.0415930376660225 0.0 2 4.0539023066298735E-5 0.0 0.0 0.13008972501975263 0.0 3 4.0539023066298735E-5 0.0 0.0 0.25393644048729525 0.0 4 4.0539023066298735E-5 0.0 0.0 0.42529489098854 0.0 5 4.0539023066298735E-5 0.0 0.0 0.753012353456499 0.0 6 4.0539023066298735E-5 0.0 0.0 1.436297587238964 0.0 7 4.0539023066298735E-5 0.0 0.0 1.7923112878071996 0.0 8 4.0539023066298735E-5 0.0 0.0 2.55691780186066 0.0 9 4.0539023066298735E-5 0.0 0.0 2.954159688887321 0.0 10 4.0539023066298735E-5 0.0 0.0 3.44277653390542 0.0 11 4.0539023066298735E-5 0.0 0.0 3.86182841534175 0.0 12 4.0539023066298735E-5 0.0 0.0 4.181802924404046 0.0 13 4.0539023066298735E-5 0.0 0.0 4.3664581744710365 0.0 14 4.0539023066298735E-5 0.0 0.0 4.46707602972159 0.0 15 4.0539023066298735E-5 0.0 0.0 4.560315782774078 0.0 16 4.0539023066298735E-5 0.0 0.0 4.721580016531814 0.0 17 4.0539023066298735E-5 0.0 0.0 4.911951268851152 0.0 18 4.0539023066298735E-5 0.0 0.0 5.14691544654342 0.0 19 4.0539023066298735E-5 0.0 0.0 5.299301634249637 0.0 20 4.0539023066298735E-5 0.0 0.0 5.461457726514832 0.0 21 4.0539023066298735E-5 0.0 0.0 5.6558018030946675 0.0 22 4.0539023066298735E-5 0.0 0.0 5.877752954382654 0.0 23 4.0539023066298735E-5 0.0 0.0 6.1402431287369375 0.0 24 4.0539023066298735E-5 0.0 0.0 6.338519490554205 0.0 25 4.0539023066298735E-5 0.0 0.0 6.514539928708074 0.0 26 4.0539023066298735E-5 0.0 0.0 6.681479625695093 0.0 27 4.0539023066298735E-5 0.0 0.0 6.860135100348271 0.0 28 4.0539023066298735E-5 0.0 0.0 7.041790462708355 0.0 29 4.0539023066298735E-5 0.0 0.0 7.243107251255595 0.0 30 4.0539023066298735E-5 0.0 0.0 7.500935437957255 0.0 31 4.0539023066298735E-5 0.0 0.0 7.70942763358723 0.0 32 4.0539023066298735E-5 0.0 0.0 7.924284455838612 0.0 33 4.0539023066298735E-5 0.0 0.0 8.129574068646349 0.0 34 4.0539023066298735E-5 0.0 0.0 8.33206648886251 0.0 35 4.0539023066298735E-5 0.0 0.0 8.575260088237238 0.0 36 4.0539023066298735E-5 0.0 0.0 8.79319787624166 0.0 37 4.0539023066298735E-5 0.0 0.0 9.033107814748016 0.0 38 4.0539023066298735E-5 0.0 0.0 9.277274350676333 0.0 39 4.0539023066298735E-5 0.0 0.0 9.598586647499816 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTTTTT 17530 0.0 43.292927 1 GCGTAAG 265 0.0 41.603775 1 AATCGCG 60 3.6379788E-12 41.249996 1 TACGGGT 175 0.0 41.142857 4 TCGATAG 125 0.0 39.600002 1 CGTAAGG 405 0.0 39.444447 2 CGAGGGA 1655 0.0 39.425983 4 CGTTAGG 325 0.0 38.76923 2 AGGGCGA 2130 0.0 38.66197 6 AGCGCGA 35 6.2512026E-6 38.571426 26 GACCGAT 1285 0.0 37.99611 9 ACACGCG 245 0.0 37.65306 36 TACGGGA 900 0.0 37.500004 4 TTAGCGG 540 0.0 37.5 2 ACGGGTA 240 0.0 37.499996 5 AAGGGAT 4285 0.0 37.49125 5 GAGGGAT 3860 0.0 37.480568 5 GGGCGAT 3880 0.0 37.46134 7 GCGATAT 205 0.0 37.317074 9 TTAGGGA 3300 0.0 37.159092 4 >>END_MODULE