FastQCFastQC Report
Thu 26 May 2016
SRR1547615_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547615_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2042728
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT208291.019665858596935No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC25490.12478411222639528No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCGAC207.0345774E-445.025
CGTTTTT123950.042.894311
CGTAAGG4100.042.804882
ACGTAAG1600.040.781251
ATCGACT501.0822987E-940.525
TAATGCG1250.039.6000021
GGGCGAT30450.039.014787
TCGATAG1500.039.01
ATAGGGA23650.038.720934
ATATCGG659.094947E-1238.0769272
GGATGCC18300.037.7459038
AAGGGCG7400.037.70275
GCGTAAG1850.037.70271
TATGGGC10000.037.5754
CGCAACT301.14023234E-437.50000418
TAGGGAG17850.037.4369745
GGCACCG9800.037.423478
CGGGTAT3850.037.40266
TACGGGA7000.037.2857134
ACGGGAC4800.037.0312545
Produced by FastQC (version 0.11.5)