##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547615_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2042728
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.33742818427123	31.0	31.0	33.0	30.0	34.0
2	31.73879831284439	31.0	31.0	34.0	30.0	34.0
3	31.780368213487062	31.0	31.0	34.0	30.0	34.0
4	35.62569368021587	37.0	35.0	37.0	33.0	37.0
5	35.57069810567046	37.0	35.0	37.0	33.0	37.0
6	35.62713586928852	37.0	35.0	37.0	33.0	37.0
7	35.9250580596144	37.0	35.0	37.0	35.0	37.0
8	35.95624723409088	37.0	35.0	37.0	35.0	37.0
9	37.81857398537642	39.0	38.0	39.0	35.0	39.0
10	37.16907880050599	39.0	37.0	39.0	33.0	39.0
11	36.928292459886976	39.0	37.0	39.0	33.0	39.0
12	36.454779588863516	38.0	35.0	39.0	32.0	39.0
13	36.281831452841494	39.0	35.0	39.0	32.0	39.0
14	37.3196475497472	39.0	35.0	41.0	32.0	41.0
15	37.51286808620629	39.0	35.0	41.0	32.0	41.0
16	37.574715772241824	39.0	35.0	41.0	33.0	41.0
17	37.47956605088881	39.0	35.0	41.0	32.0	41.0
18	37.36018843428983	39.0	36.0	41.0	32.0	41.0
19	37.25566595258889	39.0	35.0	41.0	32.0	41.0
20	37.05656553393305	39.0	35.0	41.0	32.0	41.0
21	36.89631561323877	38.0	35.0	41.0	32.0	41.0
22	36.85019591448299	38.0	35.0	41.0	32.0	41.0
23	36.7745147665279	38.0	35.0	40.0	31.0	41.0
24	36.69073317641899	38.0	35.0	40.0	31.0	41.0
25	36.5693650843382	38.0	35.0	40.0	31.0	41.0
26	36.48329146122244	38.0	35.0	40.0	31.0	41.0
27	36.38991877528481	38.0	35.0	40.0	31.0	41.0
28	36.40490265958072	38.0	35.0	40.0	31.0	41.0
29	36.38641708538778	38.0	35.0	40.0	31.0	41.0
30	36.356588836105445	38.0	35.0	40.0	31.0	41.0
31	36.23387352599073	38.0	35.0	40.0	30.0	41.0
32	36.0470165386679	38.0	35.0	40.0	30.0	41.0
33	35.82014688201268	38.0	35.0	40.0	29.0	41.0
34	35.61176818450621	38.0	35.0	40.0	28.0	41.0
35	35.3556836739889	38.0	34.0	40.0	27.0	41.0
36	35.21879956607047	38.0	34.0	40.0	25.0	41.0
37	35.15699789692999	38.0	34.0	40.0	25.0	41.0
38	35.05871413129893	38.0	34.0	40.0	25.0	41.0
39	34.9917306660505	38.0	34.0	40.0	24.0	41.0
40	34.910632742097825	38.0	34.0	40.0	24.0	41.0
41	34.80990812286315	38.0	34.0	40.0	23.0	41.0
42	34.776542447158896	38.0	34.0	40.0	23.0	41.0
43	34.7219414430115	38.0	34.0	40.0	23.0	41.0
44	34.58832845097341	37.0	34.0	40.0	23.0	41.0
45	34.50176724458665	37.0	34.0	40.0	23.0	41.0
46	34.46854255681618	37.0	34.0	40.0	23.0	41.0
47	34.408198742074326	37.0	33.0	40.0	23.0	41.0
48	34.28806282578983	37.0	33.0	40.0	23.0	41.0
49	34.21004607564003	36.0	33.0	40.0	23.0	41.0
50	34.13027725668812	36.0	33.0	40.0	23.0	41.0
51	33.69755004092566	36.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	4.0
10	1.0
11	4.0
12	6.0
13	13.0
14	10.0
15	38.0
16	115.0
17	317.0
18	760.0
19	1548.0
20	2705.0
21	4540.0
22	7649.0
23	11601.0
24	17655.0
25	26992.0
26	36232.0
27	40398.0
28	40963.0
29	43356.0
30	49646.0
31	58881.0
32	73489.0
33	95728.0
34	157146.0
35	231664.0
36	151497.0
37	191308.0
38	290998.0
39	507309.0
40	155.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.094826134463325	23.33130010456605	27.960991380154383	16.61288238081624
2	33.39989465068281	24.55481101742376	29.004351044289795	13.040943287603636
3	27.822500107699117	24.699813190987737	34.03566211458403	13.44202458672912
4	25.116706678520096	26.82603851320391	34.11247116600938	13.944783642266618
5	22.20041043154057	31.223931918493307	32.96841282833544	13.607244821630681
6	20.387442674697756	39.898116636184554	29.616228886077835	10.098211803039856
7	85.8602320034777	3.274885349395514	9.371634402622375	1.4932482445044077
8	86.82071230237213	2.500822429613732	8.93050861397112	1.7479566540430247
9	82.4255603291285	4.5283072440383645	10.561513818775676	2.4846186080574606
10	40.00806764287756	27.54634978323105	19.07052725570903	13.375055318182353
11	32.687416043643594	24.964850924841684	27.235442016754064	15.112291014760654
12	32.21353993287408	22.0278960292315	29.026478317230687	16.73208572066374
13	22.246280464163608	30.159375110146826	29.821053023212095	17.77329140247747
14	17.430171809462642	32.60458563254628	31.64107017674404	18.32417238124704
15	16.8657794870389	26.68382672582938	40.16995899600926	16.28043479112246
16	19.51953466149189	23.21111768184506	39.36471228670679	17.904635369956253
17	19.428333091826225	24.407214274245028	31.55163095625066	24.612821677678085
18	21.795902342357866	25.6746370539788	33.78849264317129	18.740967960492046
19	25.159639462522666	27.295068163749654	28.601066808698956	18.944225565028727
20	26.027106888435465	26.15756968132811	29.848124664664116	17.967198765572313
21	22.867165868387765	27.411530071551375	30.989784249297998	18.73151981076286
22	22.4614339256132	24.248553894595855	30.750643257447884	22.539368922343062
23	19.520073157072307	28.36966057154942	30.258752021806135	21.85151424957214
24	19.96643703909674	25.709737174993442	36.59973329782526	17.724092488084562
25	19.522765634974405	27.7129407341555	33.08570695657963	19.678586674290457
26	18.82139961854931	31.123282199098462	29.64442647283437	20.410891709517863
27	17.86982897380366	30.916401987929866	32.344100634054065	18.869668404212405
28	17.83477780693269	28.13282042445201	35.58344527514187	18.448956493473435
29	17.66990025103685	26.43176184004919	35.523819128146286	20.374518780767676
30	19.079828543007192	28.89072847682119	33.15610301518362	18.873339964987995
31	24.022630521537867	27.824703044164472	29.419677999224565	18.732988435073096
32	25.04836669395044	27.39625637872492	28.694569223117323	18.860807704207314
33	23.025434614887544	28.642530968391288	27.92221969836415	20.40981471835702
34	19.79499962794851	27.84996338230053	30.287781829005134	22.067255160745827
35	19.8171269008894	28.343519058827216	30.878511480725773	20.96084255955761
36	24.653306754496928	28.12723965207311	28.061347374687184	19.15810621874278
37	19.905293313647242	31.071782439952848	29.736851896091892	19.28607235030802
38	19.973192710923822	31.086517637198885	28.312726902455932	20.62756274942136
39	19.613820342209046	30.865538632652022	28.447742430710303	21.072898594428626
40	22.862759995457054	28.024386996212908	28.488619140678544	20.624233867651494
41	18.721043624016513	26.63643911475243	30.628062081686842	24.014455179544218
42	20.85456311364019	27.695023517570622	27.67862387943965	23.771789489349537
43	21.03319678390858	28.013568130460836	28.274591624533468	22.67864346109712
44	19.900789532429183	30.01026078851418	28.65839211094184	21.430557568114793
45	18.9046706169397	32.49316600154303	27.09807668960331	21.50408669191395
46	20.935141634128478	31.306566513015927	27.566078303131892	20.192213549723704
47	20.05396704798681	28.862530890064658	29.479353100363824	21.604148961584706
48	20.890103821947907	27.49024833458003	30.58204518663278	21.037602656839287
49	19.972458398768705	27.708241136362748	30.5052850893511	21.814015375517446
50	19.275351392843294	29.909219435969938	30.357982071034424	20.457447100152347
51	18.273945429836964	31.212574557160817	28.529544804790458	21.983935208211765
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1526.0
1	2260.5
2	2995.0
3	17952.0
4	32909.0
5	23375.5
6	13842.0
7	13813.5
8	13785.0
9	14415.5
10	15046.0
11	15566.0
12	16086.0
13	15713.5
14	15341.0
15	14786.0
16	14231.0
17	13554.0
18	12877.0
19	12659.0
20	12441.0
21	12684.0
22	12927.0
23	12976.0
24	13025.0
25	15136.0
26	20186.0
27	23125.0
28	26523.0
29	29921.0
30	36400.5
31	42880.0
32	49642.0
33	56404.0
34	61947.5
35	67491.0
36	73906.5
37	80322.0
38	86547.5
39	92773.0
40	110672.0
41	128571.0
42	143901.0
43	159231.0
44	164505.0
45	169779.0
46	169636.5
47	169494.0
48	166926.0
49	164358.0
50	155158.5
51	145959.0
52	132983.0
53	120007.0
54	105723.0
55	91439.0
56	82635.0
57	73831.0
58	68455.0
59	63079.0
60	56931.0
61	50783.0
62	43952.0
63	37121.0
64	31798.0
65	26475.0
66	23170.0
67	19865.0
68	16754.0
69	13643.0
70	11453.0
71	9263.0
72	7264.5
73	5266.0
74	4427.5
75	2876.5
76	2164.0
77	1569.0
78	974.0
79	650.0
80	326.0
81	227.0
82	128.0
83	126.5
84	125.0
85	80.0
86	35.0
87	25.5
88	16.0
89	12.5
90	9.0
91	5.5
92	2.0
93	1.0
94	0.0
95	0.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2042728.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.86229243451243
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.73080096861054	16.993735019446454
2	7.3802483203397715	3.226982940371745
3	2.7320244763501704	1.7918495412063935
4	1.5906714223690495	1.3910289521221597
5	1.0609048983621947	1.1596906566600496
6	0.7194003373984073	0.9436644332214534
7	0.601196330702383	0.9200470994659938
8	0.4782534524011367	0.8364573467367057
9	0.3654244400510554	0.7190114374002716
>10	5.118858951366343	27.724843621035177
>50	1.6082995170697338	24.570042899630096
>100	0.6096015243507733	17.986623104823217
>500	0.003633987897616479	0.5228129288436274
>1k	4.5424848720205987E-4	0.1789550570379365
>5k	0.0	0.0
>10k+	2.2712424360102993E-4	1.0342549619986623
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20829	1.019665858596935	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2549	0.12478411222639528	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.02888294476797694	0.0
2	0.0	0.0	0.0	0.08013793319521738	0.0
3	0.0	0.0	0.0	0.14896745920161666	0.0
4	0.0	0.0	0.0	0.26611472501478417	0.0
5	0.0	0.0	0.0	0.46134384998883843	0.0
6	0.0	0.0	0.0	0.8943432507901199	0.0
7	0.0	0.0	0.0	1.1101820702511542	0.0
8	0.0	0.0	0.0	1.6145076583862366	0.0
9	0.0	0.0	0.0	1.8642716994137252	0.0
10	0.0	0.0	0.0	2.21282520237643	0.0
11	0.0	0.0	0.0	2.5248099600142555	0.0
12	0.0	0.0	0.0	2.764440493301115	0.0
13	0.0	0.0	0.0	2.9031765364747533	0.0
14	0.0	0.0	0.0	2.9740131823718086	0.0
15	0.0	0.0	0.0	3.048766159762827	0.0
16	0.0	0.0	0.0	3.1730607305524767	0.0
17	0.0	0.0	0.0	3.3212938775989755	0.0
18	0.0	0.0	0.0	3.50487191637849	0.0
19	0.0	0.0	0.0	3.6223129070537046	0.0
20	0.0	0.0	0.0	3.739900760159943	0.0
21	0.0	0.0	0.0	3.9031628293145246	0.0
22	0.0	0.0	0.0	4.079985196266953	0.0
23	0.0	0.0	0.0	4.287697627878014	0.0
24	0.0	0.0	0.0	4.441658409734433	0.0
25	0.0	0.0	0.0	4.581569352356261	0.0
26	0.0	0.0	0.0	4.707283593312472	0.0
27	0.0	0.0	0.0	4.8429844795782895	0.0
28	0.0	0.0	0.0	4.98832933214799	0.0
29	0.0	0.0	0.0	5.150954997434803	0.0
30	0.0	0.0	0.0	5.346771572132951	0.0
31	0.0	0.0	0.0	5.526629095993202	0.0
32	0.0	0.0	0.0	5.693464817636024	0.0
33	0.0	0.0	0.0	5.873028616634226	0.0
34	0.0	0.0	0.0	6.055333847678203	0.0
35	0.0	0.0	0.0	6.2676479687946705	0.0
36	0.0	0.0	0.0	6.452205090447676	0.0
37	0.0	0.0	0.0	6.656245961283147	0.0
38	0.0	0.0	0.0	6.868364265824916	0.0
39	0.0	0.0	0.0	7.175013021802218	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCCGAC	20	7.0345774E-4	45.0	25
CGTTTTT	12395	0.0	42.89431	1
CGTAAGG	410	0.0	42.80488	2
ACGTAAG	160	0.0	40.78125	1
ATCGACT	50	1.0822987E-9	40.5	25
TAATGCG	125	0.0	39.600002	1
GGGCGAT	3045	0.0	39.01478	7
TCGATAG	150	0.0	39.0	1
ATAGGGA	2365	0.0	38.72093	4
ATATCGG	65	9.094947E-12	38.076927	2
GGATGCC	1830	0.0	37.745903	8
AAGGGCG	740	0.0	37.7027	5
GCGTAAG	185	0.0	37.7027	1
TATGGGC	1000	0.0	37.575	4
CGCAACT	30	1.14023234E-4	37.500004	18
TAGGGAG	1785	0.0	37.436974	5
GGCACCG	980	0.0	37.42347	8
CGGGTAT	385	0.0	37.4026	6
TACGGGA	700	0.0	37.285713	4
ACGGGAC	480	0.0	37.031254	5
>>END_MODULE