FastQCFastQC Report
Thu 26 May 2016
SRR1547614_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547614_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1873048
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133110.7106598442752134No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC100890.5386407609415241No Hit
GAGCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCT21170.11302433253178776No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG20210.10789899671551396No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTGCG453.8562575E-1045.0000041
TAGCGCA453.8562575E-1045.0000041
GTGTCAA453.8562575E-1045.00000437
GTATCAC453.8562575E-1045.00000436
CCGAAAC453.8562575E-1045.00000423
TAGTGTC453.8562575E-1045.00000427
CGCCCAT453.8562575E-1045.00000441
ATCTCGC502.1827873E-1145.038
GTCGCGA351.2124838E-745.044
GGTACGT406.8193913E-945.025
GGTACGC207.0344255E-445.021
GGTACAT800.045.08
GTCGAGT253.8917504E-545.013
CGAAACG351.2124838E-745.031
CAACGCC207.0344255E-445.031
CAACGAC351.2124838E-745.015
ACACCGT351.2124838E-745.027
TTGCTAG1000.045.01
GACGTAT351.2124838E-745.09
CGGTTCA207.0344255E-445.037