##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547614_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1873048
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.337164877782097	31.0	31.0	33.0	30.0	34.0
2	31.730626230614487	31.0	31.0	34.0	30.0	34.0
3	31.80188174568938	31.0	31.0	34.0	30.0	34.0
4	35.58411209963653	37.0	35.0	37.0	33.0	37.0
5	35.49736739261353	37.0	35.0	37.0	33.0	37.0
6	35.54705752335231	37.0	35.0	37.0	33.0	37.0
7	35.903878597878965	37.0	35.0	37.0	35.0	37.0
8	35.94309649298897	37.0	35.0	37.0	35.0	37.0
9	37.794316002579755	39.0	38.0	39.0	35.0	39.0
10	37.241141177375056	39.0	37.0	39.0	34.0	39.0
11	36.816800744027915	39.0	35.0	39.0	33.0	39.0
12	35.51523773015961	35.0	35.0	39.0	31.0	39.0
13	34.93164670633107	35.0	34.0	39.0	30.0	39.0
14	35.785345063233834	37.0	35.0	40.0	30.0	41.0
15	36.25638851753933	37.0	35.0	40.0	31.0	41.0
16	36.50507835357129	37.0	35.0	40.0	32.0	41.0
17	36.449633965600455	36.0	35.0	40.0	32.0	41.0
18	36.36833599566055	36.0	35.0	40.0	32.0	41.0
19	36.24964442982775	36.0	35.0	40.0	31.0	41.0
20	35.992738039815315	36.0	35.0	40.0	31.0	41.0
21	35.72971755128539	35.0	34.0	40.0	31.0	41.0
22	35.63274032486087	35.0	34.0	40.0	30.0	41.0
23	35.688453259072915	35.0	34.0	40.0	31.0	41.0
24	35.54334645988784	35.0	34.0	40.0	31.0	41.0
25	35.462267384498425	35.0	34.0	40.0	31.0	41.0
26	35.28727667416959	35.0	34.0	40.0	30.0	41.0
27	35.23718879601591	35.0	34.0	40.0	30.0	41.0
28	35.28636906261879	35.0	34.0	40.0	30.0	41.0
29	35.36457741606195	36.0	34.0	40.0	30.0	41.0
30	35.356327760954336	36.0	34.0	40.0	30.0	41.0
31	35.13873162887443	35.0	34.0	40.0	30.0	41.0
32	34.869551127360324	35.0	34.0	40.0	29.0	41.0
33	34.68714095954829	35.0	34.0	40.0	27.0	41.0
34	34.59981965224596	35.0	34.0	40.0	27.0	41.0
35	34.411279369242	35.0	34.0	40.0	26.0	41.0
36	34.200018365786676	35.0	33.0	40.0	24.0	41.0
37	34.11553681486006	35.0	33.0	40.0	24.0	41.0
38	34.09012048810281	35.0	33.0	40.0	24.0	41.0
39	34.101835083777885	35.0	33.0	40.0	24.0	41.0
40	33.94888331745903	35.0	33.0	40.0	23.0	41.0
41	33.93652111424801	35.0	34.0	40.0	23.0	41.0
42	33.868943561510434	35.0	33.0	40.0	23.0	41.0
43	33.75022423344196	35.0	33.0	40.0	23.0	41.0
44	33.618000179386755	35.0	33.0	39.0	23.0	41.0
45	33.508582268046524	35.0	33.0	39.0	23.0	41.0
46	33.49023943860488	35.0	33.0	39.0	23.0	41.0
47	33.454187506139725	35.0	33.0	39.0	23.0	41.0
48	33.34855486885547	35.0	33.0	39.0	23.0	41.0
49	33.378236969901465	35.0	33.0	39.0	23.0	41.0
50	33.219069132237934	35.0	33.0	39.0	22.0	41.0
51	32.78338782561899	35.0	32.0	38.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	4.0
12	8.0
13	5.0
14	13.0
15	42.0
16	149.0
17	395.0
18	1010.0
19	1981.0
20	3610.0
21	5754.0
22	8979.0
23	13381.0
24	19458.0
25	27712.0
26	35940.0
27	41274.0
28	43361.0
29	47428.0
30	54649.0
31	65178.0
32	80919.0
33	107668.0
34	208249.0
35	379395.0
36	102748.0
37	115749.0
38	175783.0
39	332044.0
40	162.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.02048212325578	23.739861445088433	28.863008315857364	15.37664811579842
2	30.76680362702931	25.864046196360157	29.580982441453717	13.788167735156815
3	28.09490199930808	24.863324378232697	32.873797147750615	14.167976474708604
4	24.924988574772243	27.829772648645417	32.23110139195578	15.014137384626554
5	23.11659925426364	31.05227415421281	31.778470172681107	14.052656418842444
6	21.110350615680964	40.37408544789029	28.05395270169264	10.46161123473611
7	85.00631056972378	4.736717905787786	8.655837971050396	1.6011335534380327
8	86.19501475669604	3.604552579538805	7.682184332702632	2.5182483310625248
9	82.72286668574431	5.140017767830829	9.920781528289718	2.2163340181351465
10	57.32730821634042	18.765349312991443	14.198141211543966	9.709201259124166
11	52.84157159880579	18.171451025280717	19.68758942643221	9.299387949481273
12	48.61322293929467	20.075566669941185	19.98987746176286	11.321332929001287
13	18.90437404700787	48.3919258876441	21.507777697101197	11.195922368246835
14	11.777861539052923	49.582872409035964	26.98756251841918	11.651703533491933
15	10.61398319744075	22.765727306507895	55.66419013287434	10.956099363177024
16	11.280437020300601	17.058185374854247	55.81159692650696	15.849780678338195
17	11.689983385369729	19.059629011109166	28.68063178306162	40.569755820459484
18	20.23941724931769	23.443499579295352	38.04408643024632	18.27299674114064
19	30.094477023546645	26.359121602863354	25.616321631906924	17.930079741683073
20	33.2778444546002	22.834065117391546	25.38103668459111	18.507053743417146
21	19.11232386997023	30.697024315447337	29.792455932789764	20.39819588179267
22	19.769434632748332	24.324256505973153	27.72128637386762	28.185022487410894
23	16.93672559379151	32.088125878247645	24.462427017353534	26.512721510607314
24	18.175722138460948	23.500091828933375	43.39467007786239	14.929515954743286
25	14.98482687042724	24.671604785355207	38.9133113513375	21.430256992880054
26	13.074945222973463	39.92834139861872	28.598893354575	18.39782002383281
27	15.314236474452336	39.68793111548663	29.97638074411334	15.021451665947696
28	12.036477442115737	30.01738343064353	43.61772896369981	14.328410163540925
29	12.295306900837566	23.847973997462958	43.4874066227881	20.36931247891138
30	15.399925682630663	33.51377006889306	34.60957754419534	16.476726704280935
31	30.569691753761784	27.862019553156138	24.147645975970715	17.420642717111363
32	31.40319949088331	28.266600749153252	26.03195433325788	14.298245426705563
33	27.88001161742785	29.82272744745463	22.97602624171938	19.32123469339814
34	16.366638762060557	32.22410744412316	27.198715676266705	24.210538117549575
35	16.545598404312116	27.620915214132257	32.73925708257343	23.094229298982192
36	30.71848665917798	24.57545134988532	28.673798001973257	16.032263988963443
37	16.24597981471911	34.864883334543485	32.40595008777137	16.48318676296603
38	17.185411158710295	35.3397777312701	24.88185033165194	22.592960778367665
39	16.98370783877402	33.78487897800804	30.369857045841858	18.861556137376084
40	23.602491767429346	27.070315336286093	26.687890539911418	22.639302356373143
41	14.25008862559849	23.817328760394822	30.92681020454361	31.005772409463077
42	22.827498280876945	23.623687166586226	25.64531181261772	27.903502739919105
43	22.75382157851801	24.71549047328205	26.51870106905963	26.01198687914031
44	17.336128065057597	33.156758395940734	28.925580123947704	20.581533415053965
45	14.688144671145642	42.77658661176862	21.849626918263706	20.685641798822026
46	21.941455851638615	35.76742293844045	24.6761428431092	17.614978366811744
47	20.407912664277692	29.242656888664893	26.00579376502898	24.34363668202844
48	22.799041989313675	24.16638548504897	33.493215336713206	19.54135718892415
49	19.714764384041413	23.059046004160063	33.99459063515724	23.231598976641283
50	17.32384861466444	35.159216421575955	28.68041822740261	18.836516736356998
51	15.952607728152188	37.39760006150403	23.18237439723915	23.46741781310463
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1777.0
1	2373.0
2	2969.0
3	11246.5
4	19524.0
5	14239.0
6	8954.0
7	9719.5
8	10485.0
9	11622.5
10	12760.0
11	13187.0
12	13614.0
13	13427.5
14	13241.0
15	12725.5
16	12210.0
17	11458.5
18	10707.0
19	10314.5
20	9922.0
21	9210.0
22	8498.0
23	8767.5
24	9037.0
25	9798.5
26	12678.0
27	14796.0
28	18760.0
29	22724.0
30	26490.0
31	30256.0
32	32363.0
33	34470.0
34	36405.5
35	38341.0
36	44855.0
37	51369.0
38	63667.0
39	75965.0
40	117734.0
41	159503.0
42	199005.0
43	238507.0
44	237998.0
45	237489.0
46	225481.0
47	213473.0
48	189568.5
49	165664.0
50	151604.5
51	137545.0
52	116818.0
53	96091.0
54	79643.0
55	63195.0
56	55625.0
57	48055.0
58	40807.0
59	33559.0
60	29834.5
61	26110.0
62	21851.0
63	17592.0
64	13389.5
65	9187.0
66	7364.5
67	5542.0
68	4456.5
69	3371.0
70	2868.5
71	2366.0
72	1945.0
73	1524.0
74	1247.0
75	751.5
76	533.0
77	451.0
78	369.0
79	225.5
80	82.0
81	67.0
82	52.0
83	46.0
84	40.0
85	32.5
86	25.0
87	21.5
88	18.0
89	12.0
90	6.0
91	3.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1873048.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.64388577184247
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.70959888183928	13.433213444805578
2	8.495200176959475	2.8278628270849886
3	2.980134744188117	1.4880306680089799
4	1.4076196566368269	0.9371304310105386
5	0.762944163058248	0.6349177750117717
6	0.5338173035547852	0.5330876534039282
7	0.3800800172111478	0.44282058734355845
8	0.2509254355275091	0.3341099428935747
9	0.1937938308890071	0.2902934146143952
>10	1.6964559889243427	6.48870224287152
>50	0.8122235805124894	10.049659074465247
>100	1.750229608350966	58.150022259550305
>500	0.02372641808906582	2.423711000123616
>1k	0.002600155407020911	0.7005955084947985
>5k	0.0	0.0
>10k+	6.500388517552278E-4	1.2658431703172175
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13311	0.7106598442752134	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	10089	0.5386407609415241	No Hit
GAGCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCT	2117	0.11302433253178776	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	2021	0.10789899671551396	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04943813506114098	0.0
2	0.0	0.0	0.0	0.18034775403513417	0.0
3	0.0	0.0	0.0	0.2758071336132336	0.0
4	0.0	0.0	0.0	0.6667741563483691	0.0
5	0.0	0.0	0.0	1.0461557845821357	0.0
6	0.0	0.0	0.0	1.7945615915876154	0.0
7	0.0	0.0	0.0	2.0564876073651077	0.0
8	0.0	0.0	0.0	2.832121760894542	0.0
9	0.0	0.0	0.0	3.072478655111882	0.0
10	0.0	0.0	0.0	3.4893393015021505	0.0
11	0.0	0.0	0.0	3.9197073433248906	0.0
12	0.0	0.0	0.0	4.263959065651281	0.0
13	0.0	0.0	0.0	4.42716897805075	0.0
14	0.0	0.0	0.0	4.486804395829685	0.0
15	0.0	0.0	0.0	4.567848768424515	0.0
16	0.0	0.0	0.0	4.763679307737975	0.0
17	0.0	0.0	0.0	4.949526119992654	0.0
18	0.0	0.0	0.0	5.238733871208853	0.0
19	0.0	0.0	0.0	5.357951317851971	0.0
20	0.0	0.0	0.0	5.485871157599806	0.0
21	0.0	0.0	0.0	5.673479804041327	0.0
22	5.3388914752852036E-5	0.0	0.0	5.845498887375016	0.0
23	5.3388914752852036E-5	0.0	0.0	6.047949652117832	0.0
24	5.3388914752852036E-5	0.0	0.0	6.189803998616159	0.0
25	5.3388914752852036E-5	0.0	0.0	6.300319052154563	0.0
26	5.3388914752852036E-5	0.0	0.0	6.416172997168252	0.0
27	5.3388914752852036E-5	0.0	0.0	6.513607766592207	0.0
28	5.3388914752852036E-5	0.0	0.0	6.627646488504299	0.0
29	5.3388914752852036E-5	0.0	0.0	6.747504602124452	0.0
30	5.3388914752852036E-5	0.0	0.0	6.915359350107418	0.0
31	5.3388914752852036E-5	0.0	0.0	7.0940520477852145	0.0
32	5.3388914752852036E-5	0.0	0.0	7.234358115755709	0.0
33	5.3388914752852036E-5	0.0	0.0	7.366228735195254	0.0
34	5.3388914752852036E-5	0.0	0.0	7.518440531155635	0.0
35	5.3388914752852036E-5	0.0	0.0	7.723293797062328	0.0
36	5.3388914752852036E-5	0.0	0.0	7.891201933960048	0.0
37	5.3388914752852036E-5	0.0	0.0	8.060444793726589	0.0
38	5.3388914752852036E-5	0.0	0.0	8.205555864024841	0.0
39	5.3388914752852036E-5	0.0	0.0	8.362305717739215	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATTGCG	45	3.8562575E-10	45.000004	1
TAGCGCA	45	3.8562575E-10	45.000004	1
GTGTCAA	45	3.8562575E-10	45.000004	37
GTATCAC	45	3.8562575E-10	45.000004	36
CCGAAAC	45	3.8562575E-10	45.000004	23
TAGTGTC	45	3.8562575E-10	45.000004	27
CGCCCAT	45	3.8562575E-10	45.000004	41
ATCTCGC	50	2.1827873E-11	45.0	38
GTCGCGA	35	1.2124838E-7	45.0	44
GGTACGT	40	6.8193913E-9	45.0	25
GGTACGC	20	7.0344255E-4	45.0	21
GGTACAT	80	0.0	45.0	8
GTCGAGT	25	3.8917504E-5	45.0	13
CGAAACG	35	1.2124838E-7	45.0	31
CAACGCC	20	7.0344255E-4	45.0	31
CAACGAC	35	1.2124838E-7	45.0	15
ACACCGT	35	1.2124838E-7	45.0	27
TTGCTAG	100	0.0	45.0	1
GACGTAT	35	1.2124838E-7	45.0	9
CGGTTCA	20	7.0344255E-4	45.0	37
>>END_MODULE