FastQCFastQC Report
Thu 26 May 2016
SRR1547613_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547613_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3122157
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140910.4513225952442494No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC35380.11331909317820982No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTATCG207.035148E-445.00000413
AATCGAT207.035148E-445.00000414
GCGATCG302.1667038E-644.9999969
CGTAAGG5450.040.4587172
AAGGGAT54200.040.101485
CGTTTTT77100.040.0680921
GGGCGAT41600.038.8341337
TACGTCG356.2517356E-638.571431
AGGGCGA24650.038.2454386
TAAGGGA41800.038.1100464
AGGGATT49950.038.0180176
AATGCGG4750.037.894742
AGGGATG63350.037.6835066
CCCGTTA601.5643309E-1037.49999625
TTACGCG601.5643309E-1037.4999961
TATGGGA34350.037.3362464
TAGGGAT47400.037.3101275
GACCGAT14200.037.2359129
AGGGATC31600.036.9541136
ATAGGGA45500.036.5439574
Produced by FastQC (version 0.11.5)