FastQCFastQC Report
Thu 26 May 2016
SRR1547612_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547612_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1191521
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57630.4836675140429753No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC33240.27897116374784836No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCGAA253.890893E-545.011
TTATCCG351.2119744E-745.01
CGCCTAG207.0333906E-445.044
CATAGGA207.0333906E-445.035
TTGACGC351.2119744E-745.031
ATGCGAA207.0333906E-445.011
CGACTAA207.0333906E-445.040
CGTCCAT253.890893E-545.045
TTCGATC207.0333906E-445.035
TATCGTG351.2119744E-745.01
CCCGCTA207.0333906E-445.033
CTAGACC302.1655687E-644.99999625
GGTTACG302.1655687E-644.9999961
GCGATAT1500.043.59
TACGGGA3750.043.24
CGGGATA2250.043.06
TATAGCG800.042.18751
CGGTCTA1600.042.187531
TACGGGT700.041.7857134
TTATACG556.184564E-1140.9090921