##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547612_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1191521
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.400693735150284	31.0	31.0	33.0	30.0	34.0
2	31.817074143049094	31.0	31.0	34.0	30.0	34.0
3	31.942534793763603	31.0	31.0	34.0	30.0	34.0
4	35.6949629926791	37.0	35.0	37.0	33.0	37.0
5	35.6032163931647	37.0	35.0	37.0	33.0	37.0
6	35.659285065055506	37.0	35.0	37.0	33.0	37.0
7	35.96009638101217	37.0	35.0	37.0	35.0	37.0
8	35.99681667381439	37.0	35.0	37.0	35.0	37.0
9	37.8561913722041	39.0	38.0	39.0	35.0	39.0
10	37.285383136344215	39.0	37.0	39.0	34.0	39.0
11	36.93817398098733	39.0	37.0	39.0	33.0	39.0
12	35.92406428422159	37.0	35.0	39.0	32.0	39.0
13	35.53829013504588	37.0	35.0	39.0	31.0	39.0
14	36.43222150511825	38.0	35.0	41.0	31.0	41.0
15	36.762796459315446	38.0	35.0	41.0	32.0	41.0
16	36.93697467354751	38.0	35.0	41.0	32.0	41.0
17	36.87375044166238	38.0	35.0	41.0	32.0	41.0
18	36.813464471041634	38.0	35.0	40.0	32.0	41.0
19	36.737823336726755	37.0	35.0	40.0	32.0	41.0
20	36.56314660001796	37.0	35.0	40.0	32.0	41.0
21	36.35801635053012	37.0	35.0	40.0	31.0	41.0
22	36.283148177833205	37.0	35.0	40.0	31.0	41.0
23	36.251538160049215	37.0	35.0	40.0	31.0	41.0
24	36.1721832850617	37.0	35.0	40.0	31.0	41.0
25	36.078513093768386	36.0	35.0	40.0	31.0	41.0
26	35.94408575258011	36.0	35.0	40.0	31.0	41.0
27	35.86838083424463	36.0	35.0	40.0	31.0	41.0
28	35.93222696033054	36.0	35.0	40.0	31.0	41.0
29	36.027301239340304	36.0	35.0	40.0	31.0	41.0
30	36.07024383120398	36.0	35.0	40.0	31.0	41.0
31	35.918000605948194	36.0	35.0	40.0	31.0	41.0
32	35.74055765697793	36.0	35.0	40.0	30.0	41.0
33	35.592081885254224	36.0	34.0	40.0	30.0	41.0
34	35.52959033034248	36.0	35.0	40.0	30.0	41.0
35	35.36869178134502	36.0	34.0	40.0	29.0	41.0
36	35.206933826596426	36.0	34.0	40.0	29.0	41.0
37	35.1399387841255	36.0	34.0	40.0	28.0	41.0
38	35.100973461651115	36.0	34.0	40.0	28.0	41.0
39	35.083277592253935	36.0	34.0	40.0	28.0	41.0
40	34.9678746744707	36.0	34.0	40.0	27.0	41.0
41	34.9369134073172	36.0	34.0	40.0	27.0	41.0
42	34.89276143685256	36.0	34.0	40.0	27.0	41.0
43	34.81853613994214	35.0	34.0	40.0	27.0	41.0
44	34.702943548623985	35.0	34.0	40.0	27.0	41.0
45	34.605058576391016	35.0	34.0	40.0	27.0	41.0
46	34.590361395225095	35.0	34.0	40.0	27.0	41.0
47	34.56849438658656	35.0	34.0	40.0	27.0	41.0
48	34.48554830338701	35.0	34.0	40.0	27.0	41.0
49	34.465516763867356	36.0	34.0	40.0	27.0	41.0
50	34.3540323670334	35.0	34.0	40.0	26.0	41.0
51	33.89933454802727	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	1.0
11	2.0
12	2.0
13	2.0
14	8.0
15	22.0
16	71.0
17	150.0
18	370.0
19	772.0
20	1354.0
21	2352.0
22	3777.0
23	5494.0
24	8258.0
25	12320.0
26	16552.0
27	19747.0
28	22042.0
29	25021.0
30	29724.0
31	36978.0
32	47087.0
33	63646.0
34	119821.0
35	209568.0
36	77504.0
37	94688.0
38	142345.0
39	251726.0
40	116.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.80868906213151	24.87408950408763	30.553552979762845	13.763668454018015
2	29.391173130813474	26.438140830081885	30.616917368640586	13.553768670464054
3	28.642634078627232	25.460147156449615	32.03804213270266	13.8591766322205
4	25.505383455264326	27.303757130591904	32.25188645437218	14.938972959771585
5	23.01117647108192	32.68486245731296	30.55951175010764	13.744449321497482
6	22.506443444974952	41.00892892361948	26.49848387061579	9.98614376078978
7	88.94119365080431	3.5533574313839202	6.249910828260685	1.2555380895510864
8	90.02208102081289	2.540534325454608	5.6660352608136995	1.7713493929187987
9	85.52262192609278	4.798152949045799	7.2793513500811144	2.3998737747803016
10	50.88454169083047	28.225016596434305	10.997707971575826	9.892733741159407
11	46.17501495987062	17.866491652266305	21.373689595063787	14.584803792799287
12	42.680825600220224	19.23113398756715	23.57751143286606	14.510528979346565
13	22.069690756604373	40.28254642595472	23.72538964902843	13.922373168412475
14	15.332923213271105	42.09745359083054	27.295532348989234	15.27409084690912
15	13.626281030716203	23.961726230590983	48.13570218233669	14.27629055635612
16	15.690449433958781	19.691637830974024	47.55316943637586	17.064743298691337
17	16.420692543396214	19.528904652121113	28.755347157121026	35.29505564736165
18	21.494711381503137	21.657948118413355	35.78484978443519	21.06249071564832
19	27.85347467648493	24.568429763302536	26.701753473081887	20.876342087130652
20	31.415392594843063	22.605896161293	26.65886711186794	19.319844131995996
21	22.18970542692911	27.21672551302075	30.337442646835434	20.256126413214705
22	23.86697338947446	22.786002093122992	26.168905122108633	27.178119395293916
23	19.24321938094251	28.683590133954834	25.70093183418505	26.37225865091761
24	20.081643546357974	22.880419228867975	39.76723868064432	17.270698544129733
25	16.67238764570662	24.791170277317814	36.80379951339506	21.732642563580498
26	17.116441925908145	33.99067242625182	28.299039630858374	20.593846016981658
27	16.828322790785894	35.76202181917063	29.294489983810607	18.115165406232876
28	14.731590966504157	29.14510109347632	38.84992375291748	17.27338418710203
29	14.805026516528033	24.059080788336924	38.34519072681052	22.79070196832452
30	17.932289905087696	30.861310879120047	33.2344960768631	17.97190313892915
31	28.433405705816345	28.3851480586578	24.82138376075621	18.36006247476964
32	29.24195209316495	25.543989572991162	28.04180538991759	17.172252943926292
33	27.49737520362629	28.127494186002593	24.79352021491858	19.581610395452532
34	19.272845379980712	27.444837312980635	28.147132950237552	25.135184356801098
35	19.189338668810706	26.511576380105762	31.230335008782887	23.06874994230064
36	30.034552475365523	23.6764605911268	27.280509533612918	19.00847739989476
37	19.856972726456355	31.666751991781933	30.57361137571222	17.902663906049497
38	19.521770912975935	32.725986365326335	24.408969711822117	23.343273009875613
39	19.1952974391555	32.346303590117174	28.601258391585212	19.857140579142122
40	24.293235284984487	26.48287357083929	26.250817232763836	22.97307391141239
41	17.258025666354182	23.74024461171897	29.672494232162084	29.329235489764766
42	21.88631169740189	24.257986220973024	26.225219698184087	27.630482383440995
43	21.90821647289473	25.054447214946272	28.024180857911862	25.013155454247133
44	18.1948115056302	29.38748037172656	30.07928521612292	22.33842290652032
45	17.662886344428674	36.641989524313885	23.95904058761868	21.73608354363876
46	22.336912232348404	32.79002216494716	25.597534579751425	19.275531022953015
47	20.024405780510794	28.353843532761907	27.7619949627409	23.8597557239864
48	22.523396566237608	24.05673085073616	31.94496781844382	21.474904764582412
49	21.31729109264545	23.209997977375135	33.04230475165775	22.430406178321658
50	19.778249816830755	31.76142090655557	28.81040283805321	19.64992643856046
51	18.15830354647547	32.87159857023082	25.21617327768457	23.753924605609132
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	558.0
1	1009.0
2	1460.0
3	5513.5
4	9567.0
5	6845.5
6	4124.0
7	4357.5
8	4591.0
9	4944.5
10	5298.0
11	5550.5
12	5803.0
13	5525.0
14	5247.0
15	5053.5
16	4860.0
17	4497.5
18	4135.0
19	3919.5
20	3704.0
21	4031.0
22	4358.0
23	4695.0
24	5032.0
25	5662.0
26	7698.0
27	9104.0
28	9871.0
29	10638.0
30	13074.5
31	15511.0
32	18973.5
33	22436.0
34	26973.5
35	31511.0
36	33624.0
37	35737.0
38	40881.5
39	46026.0
40	69133.0
41	92240.0
42	111826.0
43	131412.0
44	135570.0
45	139728.0
46	132641.0
47	125554.0
48	112234.5
49	98915.0
50	91554.5
51	84194.0
52	75188.5
53	66183.0
54	59789.5
55	53396.0
56	47397.0
57	41398.0
58	37022.0
59	32646.0
60	29621.0
61	26596.0
62	23588.5
63	20581.0
64	17781.0
65	14981.0
66	12725.0
67	10469.0
68	8351.0
69	6233.0
70	5238.5
71	4244.0
72	3573.5
73	2903.0
74	2317.5
75	1374.0
76	1016.0
77	844.0
78	672.0
79	457.0
80	242.0
81	170.5
82	99.0
83	85.5
84	72.0
85	41.5
86	11.0
87	8.5
88	6.0
89	3.5
90	1.0
91	1.0
92	1.0
93	1.5
94	2.0
95	1.0
96	0.0
97	1.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1191521.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.09292004250519
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.48997120226302	14.201127731010798
2	7.821539640383798	2.830289826454977
3	2.5128768130796253	1.363958377671448
4	1.1504508322483686	0.8326005968281314
5	0.6868338511356532	0.6213414975541645
6	0.4790290252182412	0.5200220310787704
7	0.35387704281173515	0.4481868328329637
8	0.30461280628805115	0.4409068118474265
9	0.2552823844224586	0.4156923392654054
>10	4.795659639028557	23.211114288581935
>50	1.9945409670157153	25.82737805705374
>100	1.151110987768786	28.06597959649594
>500	0.0023415601866736903	0.27080130659622625
>1k	0.0014049361120042141	0.462294095365773
>5k	4.6831203733473803E-4	0.48830661136234416
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5763	0.4836675140429753	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3324	0.27897116374784836	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04238280315663761	0.0
2	0.0	0.0	0.0	0.17674887811461149	0.0
3	0.0	0.0	0.0	0.27410343586055136	0.0
4	0.0	0.0	0.0	0.5841273464756391	0.0
5	0.0	0.0	0.0	0.9271343098443082	0.0
6	0.0	0.0	0.0	1.384616804907341	0.0
7	0.0	0.0	0.0	1.5709332861107776	0.0
8	0.0	0.0	0.0	2.1123421240582414	0.0
9	0.0	0.0	0.0	2.26592733153675	0.0
10	0.0	0.0	0.0	2.553626834944579	0.0
11	0.0	0.0	0.0	2.9636909462779086	0.0
12	0.0	0.0	0.0	3.3042640457029293	0.0
13	0.0	0.0	0.0	3.4340141718022594	0.0
14	0.0	0.0	0.0	3.4857967253619533	0.0
15	0.0	0.0	0.0	3.571989079504264	0.0
16	0.0	0.0	0.0	3.7651035944813396	0.0
17	0.0	0.0	0.0	3.981213927408749	0.0
18	0.0	0.0	0.0	4.276970359733483	0.0
19	0.0	0.0	0.0	4.430555567211992	0.0
20	0.0	0.0	0.0	4.588756723549144	0.0
21	0.0	0.0	0.0	4.804447424762132	0.0
22	0.0	0.0	0.0	5.028027202206256	0.0
23	8.392634288443091E-5	0.0	0.0	5.27812770400186	0.0
24	8.392634288443091E-5	0.0	0.0	5.45504443480224	0.0
25	8.392634288443091E-5	0.0	0.0	5.5946139430190485	0.0
26	8.392634288443091E-5	0.0	0.0	5.749541971983708	0.0
27	8.392634288443091E-5	0.0	0.0	5.887768658714366	0.0
28	1.6785268576886183E-4	0.0	0.0	6.038500370534804	0.0
29	1.6785268576886183E-4	0.0	0.0	6.1989675381298355	0.0
30	1.6785268576886183E-4	0.0	0.0	6.392753463849987	0.0
31	1.6785268576886183E-4	0.0	0.0	6.58846969545648	0.0
32	1.6785268576886183E-4	0.0	0.0	6.75649023391111	0.0
33	1.6785268576886183E-4	0.0	0.0	6.927112488995158	0.0
34	1.6785268576886183E-4	0.0	0.0	7.108896947682836	0.0
35	1.6785268576886183E-4	0.0	0.0	7.354633279648449	0.0
36	1.6785268576886183E-4	0.0	0.0	7.563777726116451	0.0
37	1.6785268576886183E-4	0.0	0.0	7.769900824240613	0.0
38	1.6785268576886183E-4	0.0	0.0	7.962595707503267	0.0
39	1.6785268576886183E-4	0.0	0.0	8.152353168764964	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCCGAA	25	3.890893E-5	45.0	11
TTATCCG	35	1.2119744E-7	45.0	1
CGCCTAG	20	7.0333906E-4	45.0	44
CATAGGA	20	7.0333906E-4	45.0	35
TTGACGC	35	1.2119744E-7	45.0	31
ATGCGAA	20	7.0333906E-4	45.0	11
CGACTAA	20	7.0333906E-4	45.0	40
CGTCCAT	25	3.890893E-5	45.0	45
TTCGATC	20	7.0333906E-4	45.0	35
TATCGTG	35	1.2119744E-7	45.0	1
CCCGCTA	20	7.0333906E-4	45.0	33
CTAGACC	30	2.1655687E-6	44.999996	25
GGTTACG	30	2.1655687E-6	44.999996	1
GCGATAT	150	0.0	43.5	9
TACGGGA	375	0.0	43.2	4
CGGGATA	225	0.0	43.0	6
TATAGCG	80	0.0	42.1875	1
CGGTCTA	160	0.0	42.1875	31
TACGGGT	70	0.0	41.785713	4
TTATACG	55	6.184564E-11	40.909092	1
>>END_MODULE