FastQCFastQC Report
Thu 26 May 2016
SRR1547611_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547611_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2198849
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129900.5907636222405449No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC80010.36387218949550426No Hit
GAGCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCT26310.11965350963163No Hit
AAGCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCT21990.10000686722917307No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGGTA302.166409E-645.00000439
ATCTCGA453.8562575E-1045.00000443
TCCGCGA302.166409E-645.00000417
GGTCGCT406.8193913E-945.0000048
CGGCCTA207.034692E-445.00000444
ACCGCTT406.8193913E-945.00000435
TAGTTCG207.034692E-445.00000431
CCCGTTA207.034692E-445.00000436
CTAACGG1200.045.0000042
AGTGCCG453.8562575E-1045.0000041
TCGGAAT207.034692E-445.00000441
GTTCGTC207.034692E-445.00000439
ACGGCAT453.8562575E-1045.00000443
TTGCGTA302.166409E-645.0000041
CGTACTT302.166409E-645.00000440
CTACCTA406.8193913E-945.00000421
ACGCTAA302.166409E-645.00000436
TATAGTC207.034692E-445.00000424
CATCGGC207.034692E-445.00000433
ATCGGAC406.8193913E-945.00000410