FastQCFastQC Report
Thu 26 May 2016
SRR1547610_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547610_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1645104
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119150.7242703196879954No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC57290.3482454604693685No Hit
GAGCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCT24050.14619136540911698No Hit
AAGCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCT20130.12236308464388879No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGGTA207.0341735E-445.00000412
AACCGGT207.0341735E-445.00000430
CAAACGA207.0341735E-445.00000429
TCACCGT207.0341735E-445.00000445
CACGAAC207.0341735E-445.00000427
TGCGGAT406.8175723E-945.00000438
TAGCACG406.8175723E-945.0000041
CTAACGT207.0341735E-445.00000414
TACGCGG1500.045.0000042
CGGATTA207.0341735E-445.00000420
GTATGCG1150.045.0000041
GTGTCGA207.0341735E-445.00000439
GTCAACG207.0341735E-445.0000041
CGCAGAT207.0341735E-445.00000428
ACGCTAC207.0341735E-445.00000425
CGTACCG207.0341735E-445.00000427
ACCCGGT207.0341735E-445.00000434
TTCGCAC207.0341735E-445.00000444
GCTCGAT207.0341735E-445.0000049
ATACCCG207.0341735E-445.00000428