##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547610_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1645104
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.26972154951906	31.0	31.0	33.0	30.0	34.0
2	31.65045188632451	31.0	31.0	34.0	30.0	34.0
3	31.714861188107257	31.0	31.0	34.0	30.0	34.0
4	35.4986183244342	37.0	35.0	37.0	33.0	37.0
5	35.40735175405324	37.0	35.0	37.0	33.0	37.0
6	35.46717289606006	37.0	35.0	37.0	33.0	37.0
7	35.87900096285706	37.0	35.0	37.0	35.0	37.0
8	35.924556745348625	37.0	35.0	37.0	35.0	37.0
9	37.75347272877581	39.0	37.0	39.0	35.0	39.0
10	37.15201956836771	39.0	37.0	39.0	33.0	39.0
11	36.737422679660376	39.0	35.0	39.0	32.0	39.0
12	35.43418896312938	35.0	35.0	39.0	31.0	39.0
13	34.88262018693043	35.0	34.0	39.0	30.0	39.0
14	35.71857949406238	36.0	35.0	40.0	30.0	41.0
15	36.15069199272508	37.0	35.0	40.0	31.0	41.0
16	36.4022614983612	36.0	35.0	40.0	32.0	41.0
17	36.326169044631825	36.0	35.0	40.0	32.0	41.0
18	36.27330065454829	36.0	35.0	40.0	31.0	41.0
19	36.16396653342281	36.0	35.0	40.0	31.0	41.0
20	35.926440516830546	36.0	35.0	40.0	31.0	41.0
21	35.6780835740476	35.0	34.0	40.0	31.0	41.0
22	35.59987696826462	35.0	34.0	40.0	30.0	41.0
23	35.622343025121815	35.0	34.0	40.0	31.0	41.0
24	35.497625074159444	35.0	34.0	40.0	30.0	41.0
25	35.37751047958062	35.0	34.0	40.0	30.0	41.0
26	35.22010766492574	35.0	34.0	40.0	30.0	41.0
27	35.15617857594413	35.0	34.0	40.0	30.0	41.0
28	35.251466776568535	35.0	34.0	40.0	30.0	41.0
29	35.30937193028526	36.0	34.0	40.0	30.0	41.0
30	35.32675988873652	36.0	34.0	40.0	30.0	41.0
31	35.07171157568154	35.0	34.0	40.0	29.0	41.0
32	34.82329323860376	35.0	34.0	40.0	29.0	41.0
33	34.59626686215583	35.0	34.0	40.0	27.0	41.0
34	34.50388486077476	35.0	34.0	40.0	27.0	41.0
35	34.28036829282526	35.0	34.0	40.0	25.0	41.0
36	34.12477448234276	35.0	33.0	40.0	24.0	41.0
37	34.012998570303154	35.0	33.0	40.0	24.0	41.0
38	33.994658696349894	35.0	33.0	40.0	24.0	41.0
39	33.98831563232476	35.0	33.0	40.0	23.0	41.0
40	33.828116641865805	35.0	33.0	39.0	23.0	41.0
41	33.85630574115679	35.0	33.0	39.0	23.0	41.0
42	33.83156627179802	35.0	33.0	39.0	23.0	41.0
43	33.760377459418976	35.0	33.0	39.0	23.0	41.0
44	33.62093095634076	35.0	33.0	39.0	23.0	41.0
45	33.483235710325914	35.0	33.0	39.0	23.0	41.0
46	33.465458110854996	35.0	33.0	39.0	23.0	41.0
47	33.41665572510917	35.0	33.0	39.0	23.0	41.0
48	33.330943818749454	35.0	33.0	39.0	23.0	41.0
49	33.35033529795077	35.0	33.0	39.0	23.0	41.0
50	33.181564205059374	35.0	33.0	38.0	23.0	41.0
51	32.725592424551884	35.0	32.0	38.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	1.0
12	1.0
13	6.0
14	11.0
15	42.0
16	134.0
17	314.0
18	852.0
19	1740.0
20	3018.0
21	5121.0
22	7585.0
23	11409.0
24	16860.0
25	23780.0
26	31426.0
27	36029.0
28	38755.0
29	42689.0
30	49747.0
31	59083.0
32	74155.0
33	98717.0
34	185276.0
35	328305.0
36	98512.0
37	110484.0
38	160018.0
39	260922.0
40	110.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.54505490230405	23.795273733453932	28.99859218626907	14.661079177972944
2	30.74316274229471	26.354078526342406	29.46190635971951	13.440852371643372
3	27.788516713836938	25.52264172964141	32.41922699112032	14.269614565401337
4	24.390919966154115	29.010263180929595	31.558187202754358	15.040629650161936
5	22.073437302444102	32.85044592925432	31.380569252764566	13.695547515537013
6	20.822148630117002	39.89765996557056	28.492180433577452	10.78801097073498
7	86.53975675701962	3.9225483616841244	7.976213054007528	1.5614818272887308
8	87.12148289712991	2.752409574105953	7.346404847353116	2.7797026814110235
9	82.7692960445054	4.6417734076386665	9.834454235112187	2.7544763127437535
10	57.399653760491745	20.519979283984476	12.801804627549382	9.278562327974402
11	51.4116432760482	17.407410109026543	20.09775673756796	11.083189877357299
12	47.836063859792446	18.441630437954075	21.795460955659944	11.92684474659353
13	19.538278431029283	46.55000534920589	22.73303086005505	11.17868535970978
14	13.097105107032746	48.27737334539336	26.020664954920786	12.604856592653107
15	10.562554099923165	22.96876063762534	54.16496464661201	12.303720615839485
16	12.201356266837841	17.687757126601113	54.19614808547058	15.91473852109046
17	12.987385599937754	19.002749978116885	27.5389884165378	40.47087600540756
18	19.408864120444665	22.81959073712057	38.66102082299964	19.110524319435125
19	29.17809451560509	24.94936490337389	26.462643091257455	19.409897489763566
20	32.37138807029829	21.907368774253786	26.592665266147307	19.128577889300615
21	18.96372508972077	30.639947383265735	30.604448107839993	19.7918794191735
22	21.445513475135918	22.94991684416304	26.541483091646484	29.063086589054553
23	16.47379132261547	32.00150264056254	23.9591539501454	27.565552086676586
24	18.464242990108833	22.883720421322906	42.83966241647945	15.812374172088816
25	14.836995107908072	24.21105291823496	38.96550005349206	21.986451920364914
26	13.762351802682385	38.02549869187602	28.1370053200284	20.0751441854132
27	15.189434832083565	38.87656950563611	29.13511850922495	16.79887715305537
28	12.432830994271486	29.993605267508922	41.95455120162616	15.619012536593432
29	12.652391581322517	23.291536583705348	41.43817047432867	22.61790136064346
30	16.87717007556969	33.29515945496455	32.24872105350178	17.578949415963976
31	31.276320524416697	27.403677822192396	24.55753557221914	16.762466081171766
32	31.573991674690475	26.705363308337954	26.24028632840234	15.480358688569234
33	30.069223587080206	29.25353047588481	22.790899541913458	17.886346395121524
34	19.1032299477723	28.853130258026237	26.63296666958442	25.410673124617045
35	17.31671675468542	28.04916892792188	32.42141530261917	22.212699014773534
36	34.03973244244741	22.98657106176874	26.77222838191385	16.201468113870003
37	18.494757778231648	34.044109065445106	31.40913887511063	16.051994281212618
38	18.95928768029255	34.818892908898164	24.38909637323841	21.83272303757088
39	18.90135821200362	33.759810929886505	28.784380805104114	18.554450053005766
40	24.810224763905502	26.01124305818963	26.67715840457503	22.50137377332983
41	15.332830021688599	23.337369552320098	29.99281504391212	31.336985382079185
42	22.414753109833786	24.081577821219813	25.454560927455045	28.04910814149136
43	22.954597399313357	24.379674476507258	26.680197726101206	25.985530398078176
44	18.073629387564555	31.263190655423607	29.314012974255732	21.349166982756103
45	16.421028700921035	41.28511024227039	21.43907011350042	20.854790943308142
46	23.436998511948183	34.047573891984946	25.23615528258396	17.279272313482917
47	20.996301753566947	28.292679368599188	26.365931880294497	24.345086997539365
48	23.447149845845612	23.78317723377975	33.0391270095994	19.730545910775245
49	21.790111749773875	22.03137309252181	33.985085441406746	22.193429716297572
50	18.737174063159536	34.26324414748247	28.278941635300868	18.72064015405713
51	17.454458806251765	35.47107052198524	23.667439870062925	23.407030801700074
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	885.0
1	1470.5
2	2056.0
3	9526.0
4	16996.0
5	12018.0
6	7040.0
7	7606.5
8	8173.0
9	9535.0
10	10897.0
11	11420.0
12	11943.0
13	11657.5
14	11372.0
15	10856.0
16	10340.0
17	9699.0
18	9058.0
19	8381.0
20	7704.0
21	7861.0
22	8018.0
23	7721.5
24	7425.0
25	7747.0
26	9335.5
27	10602.0
28	11603.5
29	12605.0
30	15066.5
31	17528.0
32	20779.0
33	24030.0
34	29431.5
35	34833.0
36	35908.5
37	36984.0
38	50198.0
39	63412.0
40	92550.5
41	121689.0
42	158759.0
43	195829.0
44	200484.0
45	205139.0
46	194903.5
47	184668.0
48	175715.5
49	166763.0
50	150810.0
51	134857.0
52	114575.5
53	94294.0
54	80400.5
55	66507.0
56	55265.0
57	44023.0
58	39685.5
59	35348.0
60	29869.0
61	24390.0
62	21013.5
63	17637.0
64	14759.0
65	11881.0
66	10318.5
67	8756.0
68	6845.5
69	4935.0
70	4071.0
71	3207.0
72	2668.0
73	2129.0
74	1815.5
75	1049.0
76	596.0
77	533.5
78	471.0
79	368.0
80	265.0
81	176.5
82	88.0
83	105.5
84	123.0
85	75.0
86	27.0
87	15.5
88	4.0
89	3.5
90	3.0
91	2.0
92	1.0
93	1.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1645104.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.850552601476025
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.9101500086174	14.874999335278064
2	9.271150017963127	3.495326021795787
3	3.292431967884006	1.8619248599213605
4	1.5723608662999113	1.1855948487475558
5	0.9314871355563171	0.8779523623201293
6	0.5739759987740902	0.6491858854125436
7	0.42422349676537047	0.5597793138390398
8	0.2945957759360336	0.4442634536363877
9	0.21701308370188965	0.3681734894577892
>10	2.0880151134931983	9.089475238471652
>50	0.9488688115166782	13.105872256237044
>100	1.4590694424166593	50.10591219429711
>500	0.01371858441493138	1.621378592895847
>1k	0.002286430735821897	0.6737836912641648
>5k	3.266329622602709E-4	0.3527466661109751
>10k+	3.266329622602709E-4	0.7336317903145869
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11915	0.7242703196879954	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	5729	0.3482454604693685	No Hit
GAGCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCT	2405	0.14619136540911698	No Hit
AAGCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCT	2013	0.12236308464388879	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.078643052354137E-5	0.0	0.0	0.08570886703819333	0.0
2	6.078643052354137E-5	0.0	0.0	0.3291585212849765	0.0
3	6.078643052354137E-5	0.0	0.0	0.4992997403203688	0.0
4	6.078643052354137E-5	0.0	0.0	1.038171446911563	0.0
5	6.078643052354137E-5	0.0	0.0	1.6758818895340355	0.0
6	6.078643052354137E-5	0.0	0.0	2.529870451959268	0.0
7	6.078643052354137E-5	0.0	0.0	2.8941635300868516	0.0
8	6.078643052354137E-5	0.0	0.0	3.9063791711648626	0.0
9	6.078643052354137E-5	0.0	0.0	4.200159989885138	0.0
10	1.2157286104708274E-4	0.0	0.0	4.743955397348739	0.0
11	1.2157286104708274E-4	0.0	0.0	5.465247181941081	0.0
12	1.2157286104708274E-4	0.0	0.0	6.002963946352327	0.0
13	1.2157286104708274E-4	0.0	0.0	6.213467355255352	0.0
14	1.2157286104708274E-4	0.0	0.0	6.285803207578366	0.0
15	1.2157286104708274E-4	0.0	0.0	6.41169190519262	0.0
16	1.2157286104708274E-4	0.0	0.0	6.716231922115562	0.0
17	1.2157286104708274E-4	0.0	0.0	7.040649101819702	0.0
18	1.2157286104708274E-4	0.0	0.0	7.481533082406948	0.0
19	1.2157286104708274E-4	0.0	0.0	7.681459652398876	0.0
20	1.2157286104708274E-4	0.0	0.0	7.8959141792859295	0.0
21	1.2157286104708274E-4	0.0	0.0	8.198448244001595	0.0
22	1.2157286104708274E-4	0.0	0.0	8.495268384248048	0.0
23	1.2157286104708274E-4	0.0	0.0	8.835976327332498	0.0
24	1.2157286104708274E-4	0.0	0.0	9.08526147890954	0.0
25	1.2157286104708274E-4	0.0	0.0	9.269322790534824	0.0
26	1.2157286104708274E-4	0.0	0.0	9.474355420690728	0.0
27	1.2157286104708274E-4	0.0	0.0	9.660848189536953	0.0
28	1.2157286104708274E-4	0.0	0.0	9.84308590824653	0.0
29	1.2157286104708274E-4	0.0	0.0	10.045322362598352	0.0
30	1.2157286104708274E-4	0.0	0.0	10.293452571995449	0.0
31	1.2157286104708274E-4	0.0	0.0	10.55404399964987	0.0
32	1.2157286104708274E-4	0.0	0.0	10.756280454001692	0.0
33	1.2157286104708274E-4	0.0	0.0	10.966601503613145	0.0
34	1.2157286104708274E-4	0.0	0.0	11.19205837442496	0.0
35	1.2157286104708274E-4	0.0	0.0	11.484684250965289	0.0
36	1.2157286104708274E-4	0.0	0.0	11.7196845913693	0.0
37	1.2157286104708274E-4	0.0	0.0	11.960824361256188	0.0
38	1.2157286104708274E-4	0.0	0.0	12.181904609070308	0.0
39	1.2157286104708274E-4	0.0	0.0	12.40505159552223	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTGGTA	20	7.0341735E-4	45.000004	12
AACCGGT	20	7.0341735E-4	45.000004	30
CAAACGA	20	7.0341735E-4	45.000004	29
TCACCGT	20	7.0341735E-4	45.000004	45
CACGAAC	20	7.0341735E-4	45.000004	27
TGCGGAT	40	6.8175723E-9	45.000004	38
TAGCACG	40	6.8175723E-9	45.000004	1
CTAACGT	20	7.0341735E-4	45.000004	14
TACGCGG	150	0.0	45.000004	2
CGGATTA	20	7.0341735E-4	45.000004	20
GTATGCG	115	0.0	45.000004	1
GTGTCGA	20	7.0341735E-4	45.000004	39
GTCAACG	20	7.0341735E-4	45.000004	1
CGCAGAT	20	7.0341735E-4	45.000004	28
ACGCTAC	20	7.0341735E-4	45.000004	25
CGTACCG	20	7.0341735E-4	45.000004	27
ACCCGGT	20	7.0341735E-4	45.000004	34
TTCGCAC	20	7.0341735E-4	45.000004	44
GCTCGAT	20	7.0341735E-4	45.000004	9
ATACCCG	20	7.0341735E-4	45.000004	28
>>END_MODULE