FastQCFastQC Report
Thu 26 May 2016
SRR1547609_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547609_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1975505
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123540.6253590853984171No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC55890.28291500148063403No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG22520.11399616806841795No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCACG351.2125201E-745.0000041
GATCGAC700.045.0000049
CATTACG302.1662981E-645.00000445
CGTAAGT302.1662981E-645.00000424
CGAACGC453.8562575E-1045.018
CGTATCA207.034519E-445.042
CGTGAAT253.8918282E-545.026
ATCGTTC253.8918282E-545.042
CCGTCCA207.034519E-445.034
TACGCCG253.8918282E-545.035
CGCTATA406.8193913E-945.026
TATTACG1100.045.01
CTACCGT207.034519E-445.040
ACGCTAA253.8918282E-545.025
TACACCG406.8193913E-945.01
GTACGTA207.034519E-445.026
GTTGCGA207.034519E-445.025
AAATGCG502.1827873E-1145.01
CGGTCTA1250.044.99999631
CGTAAGG4200.043.3928572
Produced by FastQC (version 0.11.5)