##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547609_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1975505
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.289689978005622	31.0	31.0	33.0	30.0	34.0
2	31.68957608307749	31.0	31.0	34.0	30.0	34.0
3	31.806651463802925	31.0	31.0	34.0	30.0	34.0
4	35.5789613288754	37.0	35.0	37.0	33.0	37.0
5	35.49023920465906	37.0	35.0	37.0	33.0	37.0
6	35.533550661729535	37.0	35.0	37.0	33.0	37.0
7	35.89321768357964	37.0	35.0	37.0	35.0	37.0
8	35.915138154547826	37.0	35.0	37.0	35.0	37.0
9	37.73697459636903	39.0	37.0	39.0	35.0	39.0
10	37.138570137762244	39.0	37.0	39.0	33.0	39.0
11	36.796756778646476	39.0	35.0	39.0	32.0	39.0
12	35.856949995064554	37.0	35.0	39.0	32.0	39.0
13	35.47700410781041	37.0	35.0	39.0	30.0	39.0
14	36.411471497161486	38.0	35.0	41.0	31.0	41.0
15	36.72214598292589	38.0	35.0	41.0	32.0	41.0
16	36.88537968772542	38.0	35.0	41.0	32.0	41.0
17	36.79576412107284	38.0	35.0	41.0	32.0	41.0
18	36.71948640980408	38.0	35.0	40.0	32.0	41.0
19	36.64778271884911	38.0	35.0	40.0	31.0	41.0
20	36.47962217255841	38.0	35.0	40.0	31.0	41.0
21	36.268916555513655	37.0	35.0	40.0	31.0	41.0
22	36.18973173947927	37.0	35.0	40.0	31.0	41.0
23	36.15889962313434	37.0	35.0	40.0	31.0	41.0
24	36.03912721051073	37.0	35.0	40.0	31.0	41.0
25	35.94869868717113	37.0	35.0	40.0	30.0	41.0
26	35.793220467677884	36.0	34.0	40.0	30.0	41.0
27	35.72796120485648	36.0	34.0	40.0	30.0	41.0
28	35.769260011996934	36.0	35.0	40.0	30.0	41.0
29	35.82833351472155	36.0	35.0	40.0	30.0	41.0
30	35.850282839071525	36.0	35.0	40.0	30.0	41.0
31	35.7210085522436	36.0	34.0	40.0	30.0	41.0
32	35.56839491674281	36.0	34.0	40.0	30.0	41.0
33	35.41944768552851	36.0	34.0	40.0	29.0	41.0
34	35.332988273884396	36.0	34.0	40.0	29.0	41.0
35	35.16448249941154	36.0	34.0	40.0	28.0	41.0
36	35.015244709580585	36.0	34.0	40.0	27.0	41.0
37	34.96691580127613	36.0	34.0	40.0	27.0	41.0
38	34.90976231393998	36.0	34.0	40.0	27.0	41.0
39	34.88435058377478	36.0	34.0	40.0	27.0	41.0
40	34.77850524296319	36.0	34.0	40.0	26.0	41.0
41	34.717842779441206	36.0	34.0	40.0	26.0	41.0
42	34.67860369880107	36.0	34.0	40.0	26.0	41.0
43	34.617724581815786	36.0	34.0	40.0	26.0	41.0
44	34.48046448882691	35.0	34.0	40.0	25.0	41.0
45	34.39448343588095	35.0	34.0	40.0	24.0	41.0
46	34.35209781802628	35.0	34.0	40.0	24.0	41.0
47	34.29849025945265	35.0	33.0	40.0	24.0	41.0
48	34.20060085902086	35.0	33.0	40.0	24.0	41.0
49	34.18025720005771	35.0	33.0	40.0	24.0	41.0
50	34.08031313512241	35.0	33.0	40.0	24.0	41.0
51	33.6298865353416	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	3.0
12	5.0
13	5.0
14	10.0
15	52.0
16	141.0
17	386.0
18	875.0
19	1779.0
20	3034.0
21	4921.0
22	7604.0
23	11422.0
24	16307.0
25	23247.0
26	31126.0
27	37154.0
28	40414.0
29	46469.0
30	53975.0
31	65160.0
32	81466.0
33	106370.0
34	191008.0
35	312466.0
36	131500.0
37	155222.0
38	232912.0
39	420288.0
40	182.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.12150564032994	25.086901830164944	27.75092950916348	14.040663020341634
2	30.68886183532818	26.954424311758256	28.789853733602293	13.566860119311263
3	28.28122429454747	25.78829210758768	32.338971554108944	13.591512043755902
4	24.969109164492117	28.148093778552823	32.01394073920339	14.868856317751664
5	23.221404147293985	32.90839557480239	29.378007142477493	14.492193135426131
6	22.082961065651567	39.86605956451641	27.37978390335636	10.67119546647566
7	87.79441206172599	3.9378285552301815	6.940655680446266	1.3271037025975636
8	88.37294767667001	3.126643567087909	6.406564397457864	2.0938443587842097
9	83.53135021171802	5.70603465949213	8.334729600785622	2.4278855280042317
10	48.86649236524332	28.808330021943757	12.626948552395465	9.698229060417463
11	44.63952255246127	19.564567034758202	22.448791574812514	13.347118837968013
12	41.716219397065565	20.43153522770127	23.58095778041564	14.271287594817528
13	21.615941240341076	39.31657981123814	24.389054950506324	14.678423997914457
14	15.640405870903894	40.6979987395628	27.80215691683899	15.859438472694324
15	14.225729623564606	25.224031323636236	46.15964019326704	14.390598859532119
16	16.22668634096092	21.126749869020834	45.499808909620576	17.146754880397673
17	17.16123219126249	21.016043998876235	28.784437397020003	33.03828641284127
18	21.530393494321707	24.373615860248393	34.31183418923263	19.784156456197277
19	27.518077656093	26.449591370307846	25.895657059840392	20.13667391375876
20	30.324499305240938	23.413557546045187	27.44680474106621	18.815138407647662
21	21.797818785576347	28.52632617988818	29.78514354557442	19.89071148896105
22	23.198017722050817	23.901989617844553	27.676467536148984	25.223525123955646
23	19.424046003426973	29.96712233074581	26.150427359080336	24.458404306746885
24	20.3661342289693	23.44559998582641	38.736373737348174	17.45189204785612
25	17.57621468940853	25.539748064418973	35.295886368295704	21.588150877876796
26	17.371963118291273	34.55810033383869	27.710382914748383	20.359553633121656
27	17.31005489735536	35.12393033679996	29.97213370758363	17.59388105826105
28	15.145899402937477	29.902733731375015	38.22015130308453	16.73121556260298
29	15.575865411628925	25.19462112219407	37.91835505351796	21.311158412659044
30	18.008559836598742	32.83054206392796	31.023307964292673	18.137590135180627
31	27.954776120536266	28.641081647477478	25.71686733265671	17.687274899329537
32	27.46234507126026	27.721418067785198	27.68289627209245	17.13334058886209
33	25.81188101270308	30.691797793475594	24.62975289862592	18.866568295195403
34	18.658975806186266	29.659150445076072	27.811369751025687	23.870503997711978
35	19.350444569869477	28.686639618730403	30.403719555252962	21.55919625614716
36	27.340806527951077	27.048779932219862	27.525215071589287	18.085198468239767
37	18.833462836084948	33.05534534207709	30.48233236564828	17.628859456189684
38	18.643992295640864	33.44871311386203	25.277688489778562	22.62960610071855
39	19.174236461056793	32.17698765632079	29.06578317949081	19.582992703131605
40	23.618112836970802	27.89995469512859	26.17816710157656	22.303765366324054
41	16.940832850334473	25.223727603827882	30.40032801739302	27.43511152844463
42	21.14957947461535	25.781458411899745	26.727090035206185	26.341872078278715
43	21.917585630003465	26.475863133730364	27.07793703382173	24.528614202444437
44	18.270568791271096	30.916398591752486	28.713518821769625	22.099513795206796
45	17.73713556786746	37.65902895715273	23.671567523240896	20.932267951738922
46	22.239275527017142	33.475642936869306	25.03106800539609	19.25401353071746
47	20.405617804055165	28.80514096395605	27.546121118397576	23.24312011359121
48	21.911814953644765	25.828433742258312	31.264259012252566	20.995492291844364
49	20.846669585751492	24.88376389834498	32.10353808266747	22.16602843323606
50	18.827236580013718	32.15673966909727	28.94029627867305	20.075727472215966
51	17.33587108106535	33.649826246959634	25.126233545346633	23.88806912662838
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	962.0
1	2061.0
2	3160.0
3	11397.5
4	19635.0
5	14015.5
6	8396.0
7	8452.0
8	8508.0
9	9268.5
10	10029.0
11	10126.0
12	10223.0
13	10219.5
14	10216.0
15	9764.5
16	9313.0
17	8633.0
18	7953.0
19	7990.0
20	8027.0
21	7735.5
22	7444.0
23	7733.0
24	8022.0
25	10087.0
26	14563.5
27	16975.0
28	20913.5
29	24852.0
30	28830.5
31	32809.0
32	38253.0
33	43697.0
34	51547.5
35	59398.0
36	63411.5
37	67425.0
38	77347.0
39	87269.0
40	116487.5
41	145706.0
42	176757.0
43	207808.0
44	217398.0
45	226988.0
46	215819.5
47	204651.0
48	183264.0
49	161877.0
50	148527.5
51	135178.0
52	119247.5
53	103317.0
54	92843.5
55	82370.0
56	73166.0
57	63962.0
58	57679.0
59	51396.0
60	47591.5
61	43787.0
62	37646.5
63	31506.0
64	26355.5
65	21205.0
66	17701.5
67	14198.0
68	11518.0
69	8838.0
70	7838.0
71	6838.0
72	5429.0
73	4020.0
74	3328.5
75	2064.5
76	1492.0
77	1115.5
78	739.0
79	545.0
80	351.0
81	203.0
82	55.0
83	62.0
84	69.0
85	48.5
86	28.0
87	16.5
88	5.0
89	7.5
90	10.0
91	6.5
92	3.0
93	4.0
94	5.0
95	3.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1975505.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.133717227582636
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.24539167441036	13.57768132538378
2	8.638267986859963	2.9601128204587623
3	2.9185969557498055	1.50019244823302
4	1.3526461505374585	0.9270342660914792
5	0.7961137626880502	0.682019404544194
6	0.5006371012173083	0.5146664715536415
7	0.34074504410339085	0.408676046265737
8	0.2569989118059241	0.35226773461433214
9	0.19187453573298274	0.29587716345803633
>10	2.6810420383393523	11.841828428322094
>50	1.4295135881176433	17.695502157440867
>100	1.6374037929031569	46.907043535185494
>500	0.008076343150955248	0.8947404738605641
>1k	0.0020938667428402496	0.5227605013963658
>5k	2.991238204057499E-4	0.2864420041474792
>10k+	2.991238204057499E-4	0.6331552190441865
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12354	0.6253590853984171	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	5589	0.28291500148063403	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	2252	0.11399616806841795	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.061996805880015E-5	0.0	0.0	0.05623878451332697	0.0
2	5.061996805880015E-5	0.0	0.0	0.20445405098949382	0.0
3	5.061996805880015E-5	0.0	0.0	0.3113634235296798	0.0
4	5.061996805880015E-5	0.0	0.0	0.6102237149488359	0.0
5	5.061996805880015E-5	0.0	0.0	0.9680056491884353	0.0
6	5.061996805880015E-5	0.0	0.0	1.429609137916634	0.0
7	5.061996805880015E-5	0.0	0.0	1.6203957975302519	0.0
8	5.061996805880015E-5	0.0	0.0	2.1502856231697716	0.0
9	5.061996805880015E-5	0.0	0.0	2.2993108091348793	0.0
10	5.061996805880015E-5	0.0	0.0	2.600550239052799	0.0
11	5.061996805880015E-5	0.0	0.0	3.005864323299612	0.0
12	5.061996805880015E-5	0.0	0.0	3.323808342676936	0.0
13	5.061996805880015E-5	0.0	0.0	3.4591155172981085	0.0
14	5.061996805880015E-5	0.0	0.0	3.512722063472378	0.0
15	5.061996805880015E-5	0.0	0.0	3.600851427862749	0.0
16	5.061996805880015E-5	0.0	0.0	3.8079883371593595	0.0
17	5.061996805880015E-5	0.0	0.0	4.037651132242136	0.0
18	5.061996805880015E-5	0.0	0.0	4.325982470305061	0.0
19	5.061996805880015E-5	0.0	0.0	4.46650350163629	0.0
20	5.061996805880015E-5	0.0	0.0	4.62894297913698	0.0
21	5.061996805880015E-5	0.0	0.0	4.849392940033055	0.0
22	5.061996805880015E-5	0.0	0.0	5.068830501567954	0.0
23	5.061996805880015E-5	0.0	0.0	5.311502628441842	0.0
24	5.061996805880015E-5	0.0	0.0	5.49975828965252	0.0
25	5.061996805880015E-5	0.0	0.0	5.654706011880506	0.0
26	5.061996805880015E-5	0.0	0.0	5.805705376599907	0.0
27	5.061996805880015E-5	0.0	0.0	5.943290449783726	0.0
28	5.061996805880015E-5	0.0	0.0	6.094796014183715	0.0
29	5.061996805880015E-5	0.0	0.0	6.2589059506303455	0.0
30	5.061996805880015E-5	0.0	0.0	6.470699896988365	0.0
31	5.061996805880015E-5	0.0	0.0	6.668927691906626	0.0
32	5.061996805880015E-5	0.0	0.0	6.853285615576777	0.0
33	1.012399361176003E-4	0.0	0.0	7.032176582696576	0.0
34	1.012399361176003E-4	0.0	0.0	7.228278338956368	0.0
35	1.012399361176003E-4	0.0	0.0	7.470393646181609	0.0
36	1.012399361176003E-4	0.0	0.0	7.692969645736153	0.0
37	1.012399361176003E-4	0.0	0.0	7.906383431072055	0.0
38	1.012399361176003E-4	0.0	0.0	8.121417055385837	0.0
39	1.012399361176003E-4	0.0	0.0	8.331996122510446	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATCACG	35	1.2125201E-7	45.000004	1
GATCGAC	70	0.0	45.000004	9
CATTACG	30	2.1662981E-6	45.000004	45
CGTAAGT	30	2.1662981E-6	45.000004	24
CGAACGC	45	3.8562575E-10	45.0	18
CGTATCA	20	7.034519E-4	45.0	42
CGTGAAT	25	3.8918282E-5	45.0	26
ATCGTTC	25	3.8918282E-5	45.0	42
CCGTCCA	20	7.034519E-4	45.0	34
TACGCCG	25	3.8918282E-5	45.0	35
CGCTATA	40	6.8193913E-9	45.0	26
TATTACG	110	0.0	45.0	1
CTACCGT	20	7.034519E-4	45.0	40
ACGCTAA	25	3.8918282E-5	45.0	25
TACACCG	40	6.8193913E-9	45.0	1
GTACGTA	20	7.034519E-4	45.0	26
GTTGCGA	20	7.034519E-4	45.0	25
AAATGCG	50	2.1827873E-11	45.0	1
CGGTCTA	125	0.0	44.999996	31
CGTAAGG	420	0.0	43.392857	2
>>END_MODULE