FastQCFastQC Report
Thu 26 May 2016
SRR1547608_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547608_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences862114
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48490.5624546173707885No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC10120.11738586776226809No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGATC207.032303E-445.00000433
GTCTACG207.032303E-445.0000041
TACGATA207.032303E-445.00000443
TATTACG207.032303E-445.0000041
GCGTGAT207.032303E-445.00000416
ATCGATC207.032303E-445.00000411
AGCGTCG253.8899918E-545.017
CGGGCTA253.8899918E-545.06
ATACCGG253.8899918E-545.02
TACTCGC302.1648684E-644.99999645
CGTATGG850.042.352942
CGTTTTT24600.041.6158521
ATGACGG650.041.5384642
ACGGGAC3200.041.484385
ATATGCG603.6379788E-1241.2499961
ACGGGCT556.002665E-1140.9090925
GCTACGA556.002665E-1140.90909210
CGGGACC2600.040.673086
TATGCGG1550.040.645162
GCGATAT950.040.263169