##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547608_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	862114
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.260255604247234	31.0	31.0	33.0	30.0	34.0
2	31.679127122399127	31.0	31.0	34.0	30.0	34.0
3	31.814671841543	31.0	31.0	34.0	30.0	34.0
4	35.58471153466943	37.0	35.0	37.0	33.0	37.0
5	35.48100135248934	37.0	35.0	37.0	33.0	37.0
6	35.52516024562877	37.0	35.0	37.0	33.0	37.0
7	35.89901799529993	37.0	35.0	37.0	35.0	37.0
8	35.93015888849966	37.0	35.0	37.0	35.0	37.0
9	37.75958631921068	39.0	37.0	39.0	35.0	39.0
10	37.0834877985974	39.0	37.0	39.0	33.0	39.0
11	36.756671391486506	39.0	35.0	39.0	32.0	39.0
12	36.23726560524478	38.0	35.0	39.0	32.0	39.0
13	36.04491401369192	38.0	35.0	39.0	32.0	39.0
14	37.01011467160956	39.0	35.0	41.0	31.0	41.0
15	37.25089141343256	39.0	35.0	41.0	32.0	41.0
16	37.3414478827626	39.0	35.0	41.0	32.0	41.0
17	37.25373906467126	39.0	35.0	41.0	32.0	41.0
18	37.16016443301002	39.0	35.0	41.0	32.0	41.0
19	37.14386380455485	39.0	35.0	41.0	32.0	41.0
20	37.00738185437193	39.0	35.0	41.0	32.0	41.0
21	36.827798875786726	38.0	35.0	41.0	31.0	41.0
22	36.79158788744876	38.0	35.0	41.0	31.0	41.0
23	36.72267240759343	38.0	35.0	40.0	31.0	41.0
24	36.65467327986786	38.0	35.0	40.0	31.0	41.0
25	36.54019538019334	38.0	35.0	40.0	31.0	41.0
26	36.43287430664622	38.0	35.0	40.0	31.0	41.0
27	36.34148036106594	38.0	35.0	40.0	30.0	41.0
28	36.38249929823666	38.0	35.0	40.0	30.0	41.0
29	36.391735895716806	38.0	35.0	40.0	30.0	41.0
30	36.40606230730506	38.0	35.0	40.0	30.0	41.0
31	36.33086459563352	38.0	35.0	40.0	30.0	41.0
32	36.23070150815322	38.0	35.0	40.0	30.0	41.0
33	36.119933094695135	38.0	35.0	41.0	30.0	41.0
34	36.03421241274356	38.0	35.0	41.0	30.0	41.0
35	35.883488726548926	38.0	35.0	41.0	29.0	41.0
36	35.78403900180255	38.0	35.0	40.0	29.0	41.0
37	35.729338579352614	38.0	35.0	40.0	29.0	41.0
38	35.65455844586679	38.0	34.0	40.0	29.0	41.0
39	35.57247417394915	38.0	34.0	40.0	28.0	41.0
40	35.508395641411695	38.0	34.0	40.0	27.0	41.0
41	35.43955323773886	38.0	34.0	40.0	27.0	41.0
42	35.39269980536217	38.0	34.0	40.0	27.0	41.0
43	35.365087447831726	38.0	34.0	40.0	27.0	41.0
44	35.26210338771902	38.0	34.0	40.0	27.0	41.0
45	35.17694991613638	38.0	34.0	40.0	27.0	41.0
46	35.145563115782835	37.0	34.0	40.0	27.0	41.0
47	35.10015728778328	37.0	34.0	40.0	27.0	41.0
48	34.96678049538692	37.0	34.0	40.0	26.0	41.0
49	34.8825816539344	37.0	34.0	40.0	26.0	41.0
50	34.81302124777002	37.0	34.0	40.0	26.0	41.0
51	34.41385014046866	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	1.0
13	2.0
14	6.0
15	15.0
16	51.0
17	152.0
18	301.0
19	571.0
20	1116.0
21	1745.0
22	2706.0
23	4040.0
24	6091.0
25	8431.0
26	11407.0
27	13729.0
28	15541.0
29	17777.0
30	21676.0
31	26488.0
32	32634.0
33	42237.0
34	68264.0
35	98691.0
36	63678.0
37	80194.0
38	122567.0
39	221953.0
40	48.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.05388150523017	25.141570604351628	29.347278898150357	13.457268992267842
2	30.30063309492712	26.281675045295632	30.30701276165333	13.110679098123915
3	28.19418313587298	25.38724577028096	32.49605040632677	13.922520687519285
4	25.286447036006837	28.55399633923124	31.784311587562662	14.375245037199258
5	23.34633238759607	32.400935375136	30.040922662200124	14.211809575067798
6	21.76173916674593	41.43964719283065	26.466917368236683	10.331696272186742
7	90.06743887699307	2.975824542925878	5.66873986502945	1.2879967150516056
8	90.76804227747142	2.0754795769469	5.528271203112349	1.6282069424693253
9	85.97575262668278	4.800293232681525	6.900131537128501	2.323822603507193
10	42.26424811567844	35.0600964605609	12.773252725277631	9.902402698483032
11	33.02521476278079	24.21199516537256	27.084121125512407	15.678668946334243
12	32.38075242949308	23.19240842858369	27.243728787608134	17.18311035431509
13	23.215491222738525	31.01503977432219	28.520010114671614	17.249458888267675
14	17.516592933185173	34.00130377189095	29.94267579461649	18.539427500307383
15	16.280677497407538	28.038983243515357	39.3906142343124	16.289725024764707
16	20.526519694611153	25.84843767761572	36.82633619219732	16.798706435575806
17	20.51271641569444	25.252460811447207	29.095804035197204	25.139018737661146
18	21.76278311221022	26.416923979891287	32.70936326286315	19.110929645035345
19	24.64233268454056	27.979594345991366	28.035967401062965	19.342105568405106
20	26.869184353809356	27.394636904168124	28.450645738266633	17.285533003755884
21	23.183824876988428	28.06403793465829	30.701044177452168	18.05109301090111
22	23.098917312559593	23.97304764799087	30.259919221819853	22.668115817629687
23	19.516444460941358	29.14162164168544	29.387064819733816	21.954869077639383
24	19.629886534727426	26.94608833634531	35.61095168388403	17.81307344504323
25	19.502757175965126	28.36840603446876	32.31881166527861	19.810025124287506
26	18.26046207346128	33.28214134093635	28.408191956052214	20.04920462955015
27	17.925007597603102	32.752629002660896	30.659054370999662	18.66330902873634
28	17.431105399053955	29.314104631174065	34.27852928962991	18.97626068014207
29	17.265002076291537	26.405672567665068	35.04188541190608	21.28743994413732
30	19.114177475368688	29.919477006521177	31.951458855789372	19.01488666232076
31	24.02211308481245	28.350194985813943	28.44333812001661	19.184353809357
32	24.737679703612283	27.20661072665564	28.33256390686151	19.72314566287057
33	23.517771431620414	28.54448483611216	28.00766487958669	19.930078852680737
34	19.59914814050114	28.40900391363555	29.52590956648425	22.46593837937906
35	19.36089658676231	28.21877385125401	31.06677307177473	21.353556490208952
36	24.83024286811257	27.305669551822614	28.495419399290583	19.368668180774236
37	19.709342383953864	30.572870873225582	30.812166372428706	18.90562037039185
38	20.244654419253138	30.580294485416083	28.177596002384835	20.997455092945945
39	19.491737751619855	30.756257293119006	28.892698645422765	20.859306309838374
40	22.698622223975022	27.867312211610066	28.364810222313984	21.069255342100927
41	18.47435489970004	26.7863646803091	30.107155202212237	24.632125217778622
42	21.041764778207988	26.393841185736456	29.19300695731655	23.371387078739005
43	20.487661724551508	27.46608917150168	29.22049752120949	22.82575158273732
44	19.48825793340556	29.55142823338909	29.399708159245762	21.560605673959593
45	18.421461662842734	33.364613032615175	26.636500509213402	21.577424795328692
46	21.19162894930369	31.135557478477327	27.6564352278237	20.01637834439529
47	19.96963278638324	28.36144639804017	29.433926371686344	22.23499444389025
48	21.079462808862864	26.562612369129834	31.539216391335717	20.81870843067158
49	20.431636651301336	26.85317719002359	31.151912624084517	21.56327353459055
50	19.381543508167134	30.240780221641224	30.01702791046196	20.360648359729687
51	18.97254887404682	30.563823345868414	28.05557037700351	22.408057403081262
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	452.0
1	779.0
2	1106.0
3	4316.0
4	7526.0
5	5334.5
6	3143.0
7	3139.0
8	3135.0
9	3470.5
10	3806.0
11	3996.0
12	4186.0
13	4134.0
14	4082.0
15	3894.5
16	3707.0
17	3562.0
18	3417.0
19	3333.0
20	3249.0
21	3354.0
22	3459.0
23	4311.0
24	5163.0
25	6468.0
26	9367.0
27	10961.0
28	13363.0
29	15765.0
30	17195.5
31	18626.0
32	22818.0
33	27010.0
34	30678.5
35	34347.0
36	36574.5
37	38802.0
38	42267.0
39	45732.0
40	53037.5
41	60343.0
42	67264.0
43	74185.0
44	74851.0
45	75517.0
46	74099.0
47	72681.0
48	70742.5
49	68804.0
50	67576.0
51	66348.0
52	59759.5
53	53171.0
54	47456.5
55	41742.0
56	36325.5
57	30909.0
58	26952.5
59	22996.0
60	19494.5
61	15993.0
62	13717.0
63	11441.0
64	9811.5
65	8182.0
66	6878.5
67	5575.0
68	4430.5
69	3286.0
70	2841.5
71	2397.0
72	1809.5
73	1222.0
74	1025.0
75	674.5
76	521.0
77	397.0
78	273.0
79	200.5
80	128.0
81	93.0
82	58.0
83	57.0
84	56.0
85	30.5
86	5.0
87	3.5
88	2.0
89	2.5
90	3.0
91	1.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	862114.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.275533544195657
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.87548997900332	18.17642468623381
2	6.978581837450506	3.3881759497188866
3	2.6121892177083623	1.902368609347967
4	1.562329912348345	1.5170556877725003
5	1.1176815812295118	1.356615835843333
6	0.9168847872252717	1.3354720445069868
7	0.7605074558029206	1.2923206977768287
8	0.6565945957168273	1.2751347306609142
9	0.5792422875339719	1.2655274023122804
>10	8.822189614182907	50.41326990058914
>50	1.085929006202027	16.2115636288304
>100	0.03092988713648338	1.1128849402298655
>500	4.832794865075528E-4	0.06558113868738084
>1k	9.665589730151056E-4	0.6876047474896944
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4849	0.5624546173707885	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1012	0.11738586776226809	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.038741976119167534	0.0
2	0.0	0.0	0.0	0.12840529210754031	0.0
3	0.0	0.0	0.0	0.20797713527445327	0.0
4	0.0	0.0	0.0	0.3721085610487708	0.0
5	0.0	0.0	0.0	0.6639493152877693	0.0
6	0.0	0.0	0.0	1.1032183678724623	0.0
7	0.0	0.0	0.0	1.3166472183493134	0.0
8	0.0	0.0	0.0	1.8410558232437937	0.0
9	0.0	0.0	0.0	2.0360416371848733	0.0
10	0.0	0.0	0.0	2.3458614521977372	0.0
11	0.0	0.0	0.0	2.7255096193774837	0.0
12	0.0	0.0	0.0	3.022570100937927	0.0
13	0.0	0.0	0.0	3.1612988537478803	0.0
14	0.0	0.0	0.0	3.2255594967718886	0.0
15	0.0	0.0	0.0	3.311627000605488	0.0
16	0.0	0.0	0.0	3.465782947498823	0.0
17	0.0	0.0	0.0	3.677587882809002	0.0
18	0.0	0.0	0.0	3.91061970922639	0.0
19	0.0	0.0	0.0	4.05920794697685	0.0
20	0.0	0.0	0.0	4.208144166548739	0.0
21	0.0	0.0	0.0	4.4115975381446075	0.0
22	0.0	0.0	0.0	4.6459052979072375	0.0
23	0.0	0.0	0.0	4.891812451717522	0.0
24	0.0	0.0	0.0	5.079838629230009	0.0
25	0.0	0.0	0.0	5.243274091361467	0.0
26	0.0	0.0	0.0	5.403693711040535	0.0
27	0.0	0.0	0.0	5.567129173171994	0.0
28	0.0	0.0	0.0	5.738452223255857	0.0
29	0.0	0.0	0.0	5.937265837232663	0.0
30	0.0	0.0	0.0	6.176445342495308	0.0
31	0.0	0.0	0.0	6.37908675650784	0.0
32	0.0	0.0	0.0	6.574652540151303	0.0
33	0.0	0.0	0.0	6.75861892974711	0.0
34	0.0	0.0	0.0	6.974367659033492	0.0
35	0.0	0.0	0.0	7.217026982510434	0.0
36	0.0	0.0	0.0	7.424772129903934	0.0
37	0.0	0.0	0.0	7.6421447743569875	0.0
38	0.0	0.0	0.0	7.887819940286319	0.0
39	0.0	0.0	0.0	8.273847774192276	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGATC	20	7.032303E-4	45.000004	33
GTCTACG	20	7.032303E-4	45.000004	1
TACGATA	20	7.032303E-4	45.000004	43
TATTACG	20	7.032303E-4	45.000004	1
GCGTGAT	20	7.032303E-4	45.000004	16
ATCGATC	20	7.032303E-4	45.000004	11
AGCGTCG	25	3.8899918E-5	45.0	17
CGGGCTA	25	3.8899918E-5	45.0	6
ATACCGG	25	3.8899918E-5	45.0	2
TACTCGC	30	2.1648684E-6	44.999996	45
CGTATGG	85	0.0	42.35294	2
CGTTTTT	2460	0.0	41.615852	1
ATGACGG	65	0.0	41.538464	2
ACGGGAC	320	0.0	41.48438	5
ATATGCG	60	3.6379788E-12	41.249996	1
ACGGGCT	55	6.002665E-11	40.909092	5
GCTACGA	55	6.002665E-11	40.909092	10
CGGGACC	260	0.0	40.67308	6
TATGCGG	155	0.0	40.64516	2
GCGATAT	95	0.0	40.26316	9
>>END_MODULE