FastQCFastQC Report
Thu 26 May 2016
SRR1547607_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547607_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2118475
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT188280.8887525224512915No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT92350.042.3199771
TACGGGA10650.040.352114
TAGGGAC27800.039.739215
TACGACG403.4600635E-739.3750041
GCGTAAG1550.039.1935461
TCGTCCC8100.038.6111138
TCGTAAG1400.038.571431
AGGGCGA19550.038.43996
CATACGA8600.038.19767418
AGGGATC30400.038.1167766
TAACGGG5500.038.0454563
ACGGGAA8700.038.017245
TATGGGA31200.038.0048074
ACGGGAT11900.037.626055
CGGTATG601.5643309E-1037.5000041
TTGGGAC18800.037.4601065
CGAGGGA15450.037.4271854
AAGGGAT43050.037.4216045
ACATACG8900.037.41573317
CGTAAGG3850.037.4025962
Produced by FastQC (version 0.11.5)