##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547607_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2118475
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.278109961174902	31.0	31.0	33.0	30.0	34.0
2	31.687895774082772	31.0	31.0	34.0	30.0	34.0
3	31.784940110220795	31.0	31.0	34.0	30.0	34.0
4	35.58437083279246	37.0	35.0	37.0	33.0	37.0
5	35.48958661301172	37.0	35.0	37.0	33.0	37.0
6	35.54567884917216	37.0	35.0	37.0	33.0	37.0
7	35.91529614463234	37.0	35.0	37.0	35.0	37.0
8	35.95894074747165	37.0	35.0	37.0	35.0	37.0
9	37.79428220772017	39.0	38.0	39.0	35.0	39.0
10	37.153116746716385	39.0	37.0	39.0	33.0	39.0
11	36.773911422131484	39.0	37.0	39.0	32.0	39.0
12	36.39735894924415	38.0	35.0	39.0	32.0	39.0
13	36.26759815433272	39.0	35.0	39.0	32.0	39.0
14	37.30892033184248	39.0	35.0	41.0	32.0	41.0
15	37.50773126895526	39.0	35.0	41.0	32.0	41.0
16	37.55429731292557	39.0	35.0	41.0	32.0	41.0
17	37.4572732744073	39.0	35.0	41.0	32.0	41.0
18	37.3559508608787	39.0	36.0	41.0	32.0	41.0
19	37.30738290515583	39.0	36.0	41.0	32.0	41.0
20	37.158471542029055	39.0	35.0	41.0	32.0	41.0
21	37.00260375978003	39.0	35.0	41.0	32.0	41.0
22	36.95948170263987	39.0	35.0	41.0	31.0	41.0
23	36.87166239865941	38.0	35.0	41.0	31.0	41.0
24	36.78649453026352	38.0	35.0	41.0	31.0	41.0
25	36.68220063961104	38.0	35.0	40.0	31.0	41.0
26	36.57171550289713	38.0	35.0	40.0	31.0	41.0
27	36.49746822596443	38.0	35.0	40.0	31.0	41.0
28	36.508364271468864	38.0	35.0	40.0	31.0	41.0
29	36.5246745890322	38.0	35.0	40.0	31.0	41.0
30	36.51455504549263	38.0	35.0	40.0	30.0	41.0
31	36.432280768005285	38.0	35.0	41.0	30.0	41.0
32	36.30773268506827	38.0	35.0	41.0	30.0	41.0
33	36.16118764677421	38.0	35.0	41.0	30.0	41.0
34	36.04543929005535	38.0	35.0	41.0	30.0	41.0
35	35.86383365392558	38.0	35.0	41.0	29.0	41.0
36	35.77228666847614	38.0	35.0	41.0	28.0	41.0
37	35.74780490683157	38.0	35.0	41.0	28.0	41.0
38	35.65879134754953	38.0	35.0	40.0	28.0	41.0
39	35.59597351868679	38.0	35.0	40.0	27.0	41.0
40	35.54056243288215	38.0	35.0	40.0	27.0	41.0
41	35.4619072682	38.0	34.0	40.0	27.0	41.0
42	35.44214446712848	38.0	34.0	40.0	27.0	41.0
43	35.399638419145845	38.0	34.0	40.0	27.0	41.0
44	35.27838846339938	38.0	34.0	40.0	27.0	41.0
45	35.19091799525602	38.0	34.0	40.0	26.0	41.0
46	35.16133161826314	38.0	34.0	40.0	26.0	41.0
47	35.11188850470268	38.0	34.0	40.0	26.0	41.0
48	34.97785293666435	38.0	34.0	40.0	26.0	41.0
49	34.911431100201796	37.0	34.0	40.0	26.0	41.0
50	34.83463340374562	37.0	34.0	40.0	26.0	41.0
51	34.42920072221763	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	0.0
11	2.0
12	0.0
13	4.0
14	13.0
15	24.0
16	132.0
17	271.0
18	724.0
19	1437.0
20	2547.0
21	4306.0
22	6497.0
23	10024.0
24	15003.0
25	22209.0
26	29982.0
27	35219.0
28	38407.0
29	42782.0
30	51269.0
31	62661.0
32	77019.0
33	98465.0
34	154154.0
35	221551.0
36	158473.0
37	202343.0
38	314031.0
39	568759.0
40	165.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.71281228242014	23.92928875724283	29.396381831270133	14.9615171290669
2	31.566999846587755	25.696786603570963	29.74384875913098	12.992364790710297
3	27.546230189169098	26.044442346499252	33.12972775227463	13.279599712057022
4	24.31305538181947	28.54935743872361	32.960549451846255	14.177037727610664
5	22.52020911268719	32.94846528752994	31.519607264659722	13.011718335123144
6	21.92959558172742	40.694131391685055	27.52432764134578	9.851945385241741
7	89.74559529850482	2.6966095894452375	6.469229044477749	1.0885660675721922
8	90.66073472663119	1.9086371092413175	6.1875169638537155	1.2431112002737819
9	85.89910194833547	4.942659224206092	7.328432008874309	1.8298068185841228
10	41.97141811916591	37.20416809261379	12.28308098986299	8.54133279835731
11	28.436446028393064	24.387306907091187	30.893779723621943	16.282467340893803
12	30.21947391401834	22.723846162923802	29.846469748285916	17.210210174771948
13	22.411168411239217	30.156268070192	30.13804741618381	17.29451610238497
14	17.371033408465998	33.05099186915116	30.788184897154792	18.789789825228056
15	16.630736732791277	27.829783216700694	38.46823776537367	17.071242285134353
16	20.5932097381371	25.945550454926302	36.766353154981765	16.69488665195483
17	20.072032948229268	25.78534087020144	30.14857385619372	23.994052325375563
18	21.075443420384946	26.037786615371907	33.975052809214176	18.91171715502897
19	24.440411144809357	27.904223557039852	28.91546985449439	18.739895443656405
20	25.49480168517448	27.667921500135712	29.824283977861434	17.012992836828378
21	22.74442700527502	28.08175692420255	31.17327322720353	18.000542843318897
22	22.940417045280213	24.73977743423925	30.94490140313197	21.374904117348564
23	19.78196580087091	29.026917948052255	30.427028876904377	20.764087374172462
24	19.399520881766367	27.07721356164222	35.40693187316348	18.11633368342794
25	19.30950329836321	28.7642761892399	32.527502094667156	19.398718417729736
26	18.770908318483816	32.906595546324596	29.038246852098798	19.28424928309279
27	17.879229162487167	31.977955840876106	31.375635775734906	18.767179220901827
28	17.062509588265144	29.910478056148882	34.889484180837634	18.137528174748343
29	18.031414106845727	26.56273970662859	35.20178430238733	20.204061884138355
30	19.184743742550655	29.88012603405752	32.48232808978157	18.452802133610263
31	23.385265344174467	28.304983537686308	29.443727209431316	18.866023908707916
32	24.017087763603538	28.43918384687098	29.282951179504124	18.26077721002136
33	23.063430061718925	28.54657241647883	29.260859816613365	19.129137705188874
34	19.71432280295968	28.81487862731446	29.817722654267808	21.653075915458054
35	19.490671355574175	28.74350653182124	31.11403249979348	20.651789612811104
36	23.15722394647093	27.831812978675696	29.565512927931646	19.445450146921726
37	19.93164894558586	30.678341731670187	30.87046106279281	18.519548259951144
38	19.79636294976339	30.93022103163833	28.995338628022516	20.27807739057577
39	19.291518663189322	30.55221326661868	29.589964479165438	20.566303591026564
40	22.050154002289382	28.6007623408348	28.94251761290551	20.406566043970308
41	17.980575649936863	28.032098561465208	30.69028428468592	23.297041503912013
42	20.136513293760842	27.849136761113535	29.43541934646385	22.578930598661774
43	21.25812199813545	28.011045681445378	28.981885554467247	21.748946765951924
44	20.042200167573373	30.048029832780653	29.425223332821957	20.484546666824013
45	18.732012414590685	32.502720117065344	27.579461641038954	21.185805827305018
46	20.708906170712424	31.069424940110217	28.447538913605307	19.77412997557205
47	19.584984481761644	29.29262795171055	30.245105559423642	20.877282007104167
48	20.91244881341531	27.578564769468606	31.587108651270373	19.921877765845714
49	20.377724542418484	27.140324997934833	31.40447727728673	21.077473182359952
50	18.844593398553204	30.70614475035108	30.394694296604868	20.05456755449085
51	18.618109725156067	30.832698049304337	28.865056231487273	21.684135994052326
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	952.0
1	1601.5
2	2251.0
3	15263.5
4	28276.0
5	19393.5
6	10511.0
7	10335.5
8	10160.0
9	10676.5
10	11193.0
11	11817.5
12	12442.0
13	12246.0
14	12050.0
15	11648.0
16	11246.0
17	10883.0
18	10520.0
19	10385.5
20	10251.0
21	10963.5
22	11676.0
23	12836.0
24	13996.0
25	16714.5
26	24059.5
27	28686.0
28	34481.5
29	40277.0
30	47387.5
31	54498.0
32	61358.5
33	68219.0
34	76752.0
35	85285.0
36	91732.5
37	98180.0
38	107465.5
39	116751.0
40	131339.0
41	145927.0
42	159840.5
43	173754.0
44	174373.5
45	174993.0
46	175231.5
47	175470.0
48	172249.0
49	169028.0
50	162238.0
51	155448.0
52	143559.5
53	131671.0
54	115403.5
55	99136.0
56	87142.0
57	75148.0
58	64502.0
59	53856.0
60	47001.0
61	40146.0
62	33149.5
63	26153.0
64	21387.0
65	16621.0
66	13116.5
67	9612.0
68	8044.5
69	6477.0
70	4807.5
71	3138.0
72	2502.0
73	1866.0
74	1623.5
75	1143.5
76	906.0
77	653.5
78	401.0
79	339.0
80	277.0
81	195.0
82	113.0
83	91.5
84	70.0
85	43.5
86	17.0
87	13.0
88	9.0
89	6.0
90	3.0
91	2.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2118475.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.243235066601127
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.35130228664822	15.455973598297401
2	7.2708219608999745	2.9436991616380785
3	2.714113929495942	1.648273388169685
4	1.5173345445164639	1.2286303943728372
5	0.9912379753987433	1.0032931671469272
6	0.7229348937856357	0.8780724595650565
7	0.5526845548031885	0.7831686352392515
8	0.4637632635747439	0.751045500783811
9	0.4092637407787347	0.7456339897938392
>10	6.434145832234347	32.70679491250411
>50	2.194184493620173	31.191099702079384
>100	0.37608241663982567	9.43083584154237
>500	0.0014200717359915435	0.18718987862980516
>1k	4.733572453305145E-4	0.144213343914951
>5k	0.0	0.0
>10k+	2.3667862266525726E-4	0.9020760263224908
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18828	0.8887525224512915	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.720376686059547E-5	0.0	0.0	0.02973837312217515	0.0
2	4.720376686059547E-5	0.0	0.0	0.09327464331653666	0.0
3	4.720376686059547E-5	0.0	0.0	0.162664180601612	0.0
4	4.720376686059547E-5	0.0	0.0	0.26226412867746846	0.0
5	4.720376686059547E-5	0.0	0.0	0.4585373912838244	0.0
6	4.720376686059547E-5	0.0	0.0	0.7771628175928439	0.0
7	4.720376686059547E-5	0.0	0.0	0.9455858577514485	0.0
8	4.720376686059547E-5	0.0	0.0	1.3271811090525023	0.0
9	4.720376686059547E-5	0.0	0.0	1.4961705944134343	0.0
10	4.720376686059547E-5	0.0	0.0	1.7384675297088708	0.0
11	4.720376686059547E-5	0.0	0.0	2.022445391142213	0.0
12	4.720376686059547E-5	0.0	0.0	2.236939307756759	0.0
13	4.720376686059547E-5	0.0	0.0	2.354429483472781	0.0
14	4.720376686059547E-5	0.0	0.0	2.420278738243312	0.0
15	4.720376686059547E-5	0.0	0.0	2.4897626830621085	0.0
16	4.720376686059547E-5	0.0	0.0	2.6189121891926974	0.0
17	4.720376686059547E-5	0.0	0.0	2.7879960820873504	0.0
18	4.720376686059547E-5	0.0	0.0	2.9742149423523996	0.0
19	4.720376686059547E-5	0.0	0.0	3.100532222471353	0.0
20	4.720376686059547E-5	0.0	0.0	3.2280295967618216	0.0
21	4.720376686059547E-5	0.0	0.0	3.3904577585291307	0.0
22	4.720376686059547E-5	0.0	0.0	3.583568368755827	0.0
23	4.720376686059547E-5	0.0	0.0	3.7916425730773318	0.0
24	4.720376686059547E-5	0.0	0.0	3.9526546218388225	0.0
25	4.720376686059547E-5	0.0	0.0	4.092613790580488	0.0
26	4.720376686059547E-5	0.0	0.0	4.232997793223899	0.0
27	4.720376686059547E-5	0.0	0.0	4.376874874614995	0.0
28	4.720376686059547E-5	0.0	0.0	4.532600101488098	0.0
29	4.720376686059547E-5	0.0	0.0	4.709047782013005	0.0
30	4.720376686059547E-5	0.0	0.0	4.933926527336881	0.0
31	4.720376686059547E-5	0.0	0.0	5.128689269403698	0.0
32	4.720376686059547E-5	0.0	0.0	5.316607465275729	0.0
33	4.720376686059547E-5	0.0	0.0	5.498058745087858	0.0
34	4.720376686059547E-5	0.0	0.0	5.69485124912968	0.0
35	4.720376686059547E-5	0.0	0.0	5.92548885401055	0.0
36	4.720376686059547E-5	0.0	0.0	6.124641546395402	0.0
37	4.720376686059547E-5	0.0	0.0	6.328278596632011	0.0
38	4.720376686059547E-5	4.720376686059547E-5	0.0	6.55013630087681	0.0
39	4.720376686059547E-5	4.720376686059547E-5	0.0	6.874001345307356	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	9235	0.0	42.319977	1
TACGGGA	1065	0.0	40.35211	4
TAGGGAC	2780	0.0	39.73921	5
TACGACG	40	3.4600635E-7	39.375004	1
GCGTAAG	155	0.0	39.193546	1
TCGTCCC	810	0.0	38.61111	38
TCGTAAG	140	0.0	38.57143	1
AGGGCGA	1955	0.0	38.4399	6
CATACGA	860	0.0	38.197674	18
AGGGATC	3040	0.0	38.116776	6
TAACGGG	550	0.0	38.045456	3
ACGGGAA	870	0.0	38.01724	5
TATGGGA	3120	0.0	38.004807	4
ACGGGAT	1190	0.0	37.62605	5
CGGTATG	60	1.5643309E-10	37.500004	1
TTGGGAC	1880	0.0	37.460106	5
CGAGGGA	1545	0.0	37.427185	4
AAGGGAT	4305	0.0	37.421604	5
ACATACG	890	0.0	37.415733	17
CGTAAGG	385	0.0	37.402596	2
>>END_MODULE