FastQCFastQC Report
Thu 26 May 2016
SRR1547606_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547606_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1400118
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55070.39332399126359346No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC16210.11577595602656349No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGACG351.2121745E-745.0000041
GTAATCG351.2121745E-745.0000041
GTTACGC207.0338143E-445.045
CGGGTAT1000.042.7500046
TCGATAG1250.041.41
CACGACG1500.040.50000426
TACGTAG1000.040.5000041
CATACGA2950.040.4237318
CGTTTTT38700.040.174421
GGGCGAT21200.040.1179247
TTACGCG451.9288564E-840.01
ATCGAAG850.039.7058831
TAATCGT1250.039.60000221
CACGACC1200.039.37500427
TACTCGC403.4589357E-739.37545
CCAACGG800.039.3752
CGGTCTA1550.039.1935531
AGGGCGA13100.039.1603056
ACGTAGG2300.039.1304362
TACGGGA4900.039.0306134
Produced by FastQC (version 0.11.5)