##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547606_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1400118
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.297108529423948	31.0	31.0	33.0	30.0	34.0
2	31.706903275295367	31.0	31.0	34.0	30.0	34.0
3	31.862960836158095	31.0	31.0	34.0	30.0	34.0
4	35.625934385530364	37.0	35.0	37.0	33.0	37.0
5	35.53342218298744	37.0	35.0	37.0	33.0	37.0
6	35.56140553867603	37.0	35.0	37.0	33.0	37.0
7	35.88741591780121	37.0	35.0	37.0	35.0	37.0
8	35.900359826814594	37.0	35.0	37.0	35.0	37.0
9	37.710765806881994	39.0	37.0	39.0	35.0	39.0
10	37.10376839666371	39.0	37.0	39.0	33.0	39.0
11	36.82920439562951	39.0	37.0	39.0	32.0	39.0
12	36.25970596763987	38.0	35.0	39.0	32.0	39.0
13	36.048072376756814	38.0	35.0	39.0	32.0	39.0
14	37.03480992316362	39.0	35.0	41.0	31.0	41.0
15	37.23970693898657	39.0	35.0	41.0	32.0	41.0
16	37.316498323712715	39.0	35.0	41.0	32.0	41.0
17	37.228098631686755	39.0	35.0	41.0	32.0	41.0
18	37.129304101511444	39.0	35.0	41.0	32.0	41.0
19	37.09920092449351	39.0	35.0	41.0	32.0	41.0
20	36.97172452607566	39.0	35.0	41.0	32.0	41.0
21	36.784513876687534	38.0	35.0	41.0	31.0	41.0
22	36.74682705314838	38.0	35.0	41.0	31.0	41.0
23	36.68382450622019	38.0	35.0	40.0	31.0	41.0
24	36.57852766695378	38.0	35.0	40.0	31.0	41.0
25	36.471950935564	38.0	35.0	40.0	31.0	41.0
26	36.36571417551949	38.0	35.0	40.0	31.0	41.0
27	36.265005520963236	38.0	35.0	40.0	30.0	41.0
28	36.31018099903008	38.0	35.0	40.0	30.0	41.0
29	36.32462978120416	38.0	35.0	40.0	30.0	41.0
30	36.34460024083685	38.0	35.0	40.0	30.0	41.0
31	36.274861118848555	38.0	35.0	40.0	30.0	41.0
32	36.176815097013254	38.0	35.0	40.0	30.0	41.0
33	36.07219962888842	38.0	35.0	40.0	30.0	41.0
34	35.96507651497945	38.0	35.0	40.0	30.0	41.0
35	35.80948820028026	38.0	35.0	40.0	29.0	41.0
36	35.723047628842714	38.0	35.0	40.0	29.0	41.0
37	35.68454587399062	38.0	35.0	40.0	29.0	41.0
38	35.60842229012126	38.0	34.0	40.0	28.0	41.0
39	35.545091199456046	38.0	34.0	40.0	28.0	41.0
40	35.46264814822751	38.0	34.0	40.0	27.0	41.0
41	35.39564450996273	38.0	34.0	40.0	27.0	41.0
42	35.36021892440494	38.0	34.0	40.0	27.0	41.0
43	35.31355857149183	38.0	34.0	40.0	27.0	41.0
44	35.19759405992924	37.0	34.0	40.0	27.0	41.0
45	35.104236214376215	37.0	34.0	40.0	27.0	41.0
46	35.08269874396301	37.0	34.0	40.0	27.0	41.0
47	35.03150448747891	37.0	34.0	40.0	27.0	41.0
48	34.88692381642119	37.0	34.0	40.0	26.0	41.0
49	34.82479476729819	37.0	34.0	40.0	26.0	41.0
50	34.72930852970964	37.0	34.0	40.0	26.0	41.0
51	34.29891409152657	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	4.0
11	2.0
12	7.0
13	3.0
14	14.0
15	37.0
16	99.0
17	236.0
18	520.0
19	1017.0
20	1840.0
21	2961.0
22	4677.0
23	6788.0
24	9476.0
25	13822.0
26	18437.0
27	22201.0
28	25243.0
29	29235.0
30	34850.0
31	42565.0
32	53547.0
33	68780.0
34	113574.0
35	164760.0
36	102909.0
37	132403.0
38	200867.0
39	349146.0
40	97.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.833349760520186	23.60779591434436	28.64458567063633	12.91426865449912
2	31.231296219318654	25.805753515060875	29.21825160450762	13.74469866111285
3	29.631288219992886	25.61405538676026	30.67320040168043	14.081455991566424
4	26.067945701719424	28.574305879932975	30.16381476418416	15.193933654163436
5	24.150393038301058	32.51904482336489	28.411462462449595	14.919099675884462
6	22.302477362622295	41.192242368143255	26.14886745260042	10.356412816634027
7	88.17535379160898	3.812607223105481	6.27325696834124	1.738782016944286
8	88.41776193149434	3.1730896967255617	6.249473258682483	2.1596751130976104
9	83.2081296005051	5.639881781392711	8.190166828795858	2.96182178930633
10	42.70368640357455	31.93023730856971	14.106525307152683	11.259550980703056
11	35.42165731745467	24.118395735216602	25.17173552514859	15.288211422180131
12	34.45252471577396	22.49538967429888	26.777314483493537	16.274771126433627
13	24.173462522444535	31.584552159175157	27.44718659427277	16.79479872410754
14	18.61228839283546	34.11269621560468	29.586220589978847	17.68879480158101
15	17.845710147287587	26.473697216948857	39.18576862807278	16.49482400769078
16	21.127576389989986	24.45258185381518	37.398205008435006	17.021636747759832
17	21.41119534210688	24.12368100402966	28.450102062826133	26.01502159103733
18	22.70772891999103	24.932255709875882	32.96600715082586	19.394008219307228
19	26.929658785902333	26.681608264446282	27.643812878628804	18.744920071022587
20	28.63801479589577	26.463626637183435	27.196064903101025	17.702293663819763
21	24.158606631726755	26.911588880365798	30.544211273621226	18.385593214286224
22	24.00683370973018	24.716559604261924	29.07112114836035	22.20548553764754
23	21.36634197974742	28.165911730297015	28.505311695157122	21.962434594798438
24	20.87074089469602	25.470853170947024	34.65565045231902	19.00275548203794
25	20.279719280803477	27.501039198124726	31.773107695208548	20.44613382586325
26	19.566779371452977	31.93823663434082	28.310542397140814	20.18444159706539
27	19.023396599429475	31.766108285158822	30.194097926031947	19.016397189379752
28	18.10947363007975	29.28124629495514	33.762582868015414	18.8466972069497
29	19.071749666813798	26.012664646836907	34.03955952284022	20.876026163509074
30	20.716968141256665	29.07147826111799	30.70791176172294	19.503641835902403
31	24.860190355384333	28.132843088939648	27.802513788123573	19.20445276755245
32	26.114441782764025	27.20106448170797	27.86029463230956	18.82419910321844
33	24.983179989115204	27.841081965948582	26.967298470557484	20.20843957437873
34	20.92945023205187	27.959286288727093	28.94270340071337	22.168560078507667
35	20.21922437965943	28.11905853649478	30.555567459314144	21.106149624531646
36	26.186864250013215	26.647039749506828	28.242048170225655	18.924047830254306
37	20.92359358282659	30.276019592634334	29.753849318414588	19.046537506124483
38	21.198070448347924	30.58842183301693	27.021436764615554	21.19207095401959
39	20.210225138166926	29.48087232647534	29.353097381792104	20.955805153565628
40	23.14754899229922	26.801526728461454	28.45638724736058	21.59453703187874
41	19.08560564181019	26.338351481803677	29.93240569723409	24.64363717915204
42	21.84687290642646	26.619327799514043	28.116701592294362	23.417097701765137
43	21.47083317263259	26.456912917339825	29.108332297706337	22.963921612321247
44	19.982315776241716	29.158328083775796	28.802500932064294	22.05685520791819
45	19.130387581618123	31.946307382663463	26.97765474052901	21.945650295189406
46	21.732025443569754	30.648273931197227	27.62417167695866	19.99552894827436
47	20.807889049351555	27.994640451733353	29.018696995538946	22.178773503376146
48	21.41369513141035	26.399774876117583	31.352143176503695	20.83438681596837
49	21.511972562312607	26.05158993741956	31.026099228779287	21.410338271488545
50	19.93617680795476	30.612134120124164	28.97898605688949	20.47270301503159
51	19.587706179050624	30.455147351866056	27.541178672083355	22.415967796999965
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	689.0
1	1391.0
2	2093.0
3	6175.5
4	10258.0
5	7634.0
6	5010.0
7	4934.5
8	4859.0
9	5166.5
10	5474.0
11	5647.0
12	5820.0
13	5900.5
14	5981.0
15	5812.0
16	5643.0
17	5455.0
18	5267.0
19	5455.0
20	5643.0
21	5953.0
22	6263.0
23	7126.5
24	7990.0
25	9387.0
26	13678.0
27	16572.0
28	19427.5
29	22283.0
30	25964.0
31	29645.0
32	34328.0
33	39011.0
34	43625.5
35	48240.0
36	51911.0
37	55582.0
38	62533.5
39	69485.0
40	81403.5
41	93322.0
42	105456.0
43	117590.0
44	120130.0
45	122670.0
46	122214.0
47	121758.0
48	117040.5
49	112323.0
50	105311.5
51	98300.0
52	91718.0
53	85136.0
54	76142.0
55	67148.0
56	59939.0
57	52730.0
58	48568.5
59	44407.0
60	40306.5
61	36206.0
62	31552.5
63	26899.0
64	23200.0
65	19501.0
66	16869.0
67	14237.0
68	11943.5
69	9650.0
70	7843.0
71	6036.0
72	5104.0
73	4172.0
74	3380.0
75	1973.0
76	1358.0
77	1080.0
78	802.0
79	590.5
80	379.0
81	288.0
82	197.0
83	127.0
84	57.0
85	45.5
86	34.0
87	21.5
88	9.0
89	8.0
90	7.0
91	7.0
92	7.0
93	5.0
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1400118.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.547427562148048
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.64789293371703	15.7491702784683
2	6.988345774847462	2.871850571758433
3	2.4283046472055916	1.4968624151185308
4	1.271307751404631	1.044884157247359
5	0.892825522465414	0.917263387424754
6	0.6688160329018513	0.8245469393072408
7	0.5317583841445089	0.7648386815141935
8	0.4811403935629293	0.7908957907166154
9	0.4230240213884175	0.7822849892874368
>10	7.186813444866735	36.44840233515829
>50	2.2689518012817764	32.19428911023876
>100	0.2094091297239413	5.494062311107288
>500	7.050812448615223E-4	0.10431076179039336
>1k	3.5254062243076116E-4	0.11742204504321364
>5k	3.5254062243076116E-4	0.39891622581923347
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5507	0.39332399126359346	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1621	0.11577595602656349	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.14225515277998E-5	0.0	0.0	0.03321148646042691	0.0
2	7.14225515277998E-5	0.0	0.0	0.11534742071739668	0.0
3	7.14225515277998E-5	0.0	0.0	0.18512725356005708	0.0
4	7.14225515277998E-5	0.0	0.0	0.3516132211713584	0.0
5	7.14225515277998E-5	0.0	0.0	0.6281613406869992	0.0
6	7.14225515277998E-5	0.0	0.0	0.9852026757744704	0.0
7	7.14225515277998E-5	0.0	0.0	1.1561168415804954	0.0
8	7.14225515277998E-5	0.0	0.0	1.591508715693963	0.0
9	7.14225515277998E-5	0.0	0.0	1.7302113107609502	0.0
10	7.14225515277998E-5	0.0	0.0	1.997831611335616	0.0
11	7.14225515277998E-5	0.0	0.0	2.327089573878773	0.0
12	7.14225515277998E-5	0.0	0.0	2.6077802013830262	0.0
13	7.14225515277998E-5	0.0	0.0	2.7300556095986197	0.0
14	7.14225515277998E-5	0.0	0.0	2.7814798466986352	0.0
15	7.14225515277998E-5	0.0	0.0	2.8587590474517146	0.0
16	7.14225515277998E-5	0.0	0.0	3.03874387730177	0.0
17	7.14225515277998E-5	0.0	0.0	3.2280850614019676	0.0
18	7.14225515277998E-5	0.0	0.0	3.4592798606974555	0.0
19	7.14225515277998E-5	0.0	0.0	3.593982792878886	0.0
20	7.14225515277998E-5	0.0	0.0	3.734113838976429	0.0
21	7.14225515277998E-5	0.0	0.0	3.9345255185634356	0.0
22	7.14225515277998E-5	0.0	0.0	4.147721834873918	0.0
23	7.14225515277998E-5	0.0	0.0	4.364346433657735	0.0
24	7.14225515277998E-5	0.0	0.0	4.536831895597371	0.0
25	7.14225515277998E-5	0.0	0.0	4.687676324424085	0.0
26	7.14225515277998E-5	0.0	0.0	4.834449667813713	0.0
27	7.14225515277998E-5	0.0	0.0	4.98807957615001	0.0
28	7.14225515277998E-5	0.0	0.0	5.143066512965336	0.0
29	7.14225515277998E-5	0.0	0.0	5.328836569489143	0.0
30	7.14225515277998E-5	0.0	0.0	5.546675351648933	0.0
31	7.14225515277998E-5	0.0	0.0	5.735373732785379	0.0
32	7.14225515277998E-5	0.0	0.0	5.919358225520992	0.0
33	7.14225515277998E-5	0.0	0.0	6.107270958590633	0.0
34	7.14225515277998E-5	0.0	0.0	6.3004689604733315	0.0
35	7.14225515277998E-5	0.0	0.0	6.533806436314653	0.0
36	7.14225515277998E-5	0.0	0.0	6.727932931367214	0.0
37	7.14225515277998E-5	0.0	0.0	6.932201428736721	0.0
38	7.14225515277998E-5	0.0	0.0	7.1594679876981795	0.0
39	7.14225515277998E-5	0.0	0.0	7.479583863645779	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGACG	35	1.2121745E-7	45.000004	1
GTAATCG	35	1.2121745E-7	45.000004	1
GTTACGC	20	7.0338143E-4	45.0	45
CGGGTAT	100	0.0	42.750004	6
TCGATAG	125	0.0	41.4	1
CACGACG	150	0.0	40.500004	26
TACGTAG	100	0.0	40.500004	1
CATACGA	295	0.0	40.42373	18
CGTTTTT	3870	0.0	40.17442	1
GGGCGAT	2120	0.0	40.117924	7
TTACGCG	45	1.9288564E-8	40.0	1
ATCGAAG	85	0.0	39.705883	1
TAATCGT	125	0.0	39.600002	21
CACGACC	120	0.0	39.375004	27
TACTCGC	40	3.4589357E-7	39.375	45
CCAACGG	80	0.0	39.375	2
CGGTCTA	155	0.0	39.19355	31
AGGGCGA	1310	0.0	39.160305	6
ACGTAGG	230	0.0	39.130436	2
TACGGGA	490	0.0	39.030613	4
>>END_MODULE