FastQCFastQC Report
Thu 26 May 2016
SRR1547605_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547605_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences484715
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45490.9384896279256884No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAATG351.2099918E-745.0000041
GCGCGAC302.1628948E-645.0000049
GAACGCC207.0292444E-445.00000428
GCGATAT302.1628948E-645.0000049
GCGATAC600.045.0000049
CGATACG207.0292444E-445.00000410
TTAAGCG253.8874554E-545.01
CGAGGGT253.8874554E-545.04
TGTTACG253.8874554E-545.01
CGTTTTT25800.042.0348851
TTAGCGG700.041.7857172
CGACGTT650.041.53846427
TCGACGT650.041.53846426
GGCGATA1700.041.029418
AACGGGA2050.040.6097564
GGCCGAT1000.040.58
CATACGA1000.040.518
AATACGG501.0786607E-940.52
ACGGGAT2900.040.3448265
GGACTCG451.9243089E-840.08
Produced by FastQC (version 0.11.5)