##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547605_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	484715
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.28551416811941	31.0	31.0	33.0	30.0	34.0
2	31.696671239800708	31.0	31.0	34.0	30.0	34.0
3	31.774710912598124	31.0	31.0	34.0	30.0	34.0
4	35.57729799985559	37.0	35.0	37.0	33.0	37.0
5	35.494098594019164	37.0	35.0	37.0	33.0	37.0
6	35.54163374354002	37.0	35.0	37.0	33.0	37.0
7	35.91712449583776	37.0	35.0	37.0	35.0	37.0
8	35.955388217818715	37.0	35.0	37.0	35.0	37.0
9	37.76144951156866	39.0	38.0	39.0	35.0	39.0
10	37.13392612153533	39.0	37.0	39.0	33.0	39.0
11	36.818002331266825	39.0	37.0	39.0	32.0	39.0
12	36.494110972427094	39.0	35.0	39.0	32.0	39.0
13	36.386422949568306	39.0	35.0	39.0	32.0	39.0
14	37.441354197827586	40.0	36.0	41.0	32.0	41.0
15	37.60525669723446	40.0	36.0	41.0	32.0	41.0
16	37.64654075075044	40.0	36.0	41.0	32.0	41.0
17	37.53648019970498	39.0	36.0	41.0	32.0	41.0
18	37.40424166778416	39.0	36.0	41.0	32.0	41.0
19	37.34387629844341	39.0	36.0	41.0	32.0	41.0
20	37.19333216426147	39.0	35.0	41.0	32.0	41.0
21	37.038606191267036	39.0	35.0	41.0	31.0	41.0
22	36.984066925925546	39.0	35.0	41.0	31.0	41.0
23	36.90514838616507	39.0	35.0	41.0	31.0	41.0
24	36.8231661904418	38.0	35.0	41.0	31.0	41.0
25	36.73491845723776	38.0	35.0	41.0	31.0	41.0
26	36.638707281598464	38.0	35.0	40.0	31.0	41.0
27	36.54676871976316	38.0	35.0	40.0	31.0	41.0
28	36.546112664142846	38.0	35.0	40.0	31.0	41.0
29	36.54451378645183	38.0	35.0	40.0	31.0	41.0
30	36.52414099006633	38.0	35.0	40.0	30.0	41.0
31	36.43881662420185	38.0	35.0	41.0	30.0	41.0
32	36.31527598692015	38.0	35.0	41.0	30.0	41.0
33	36.163529084100965	38.0	35.0	41.0	30.0	41.0
34	36.0019392839091	38.0	35.0	41.0	29.0	41.0
35	35.79822369846198	38.0	35.0	41.0	28.0	41.0
36	35.69500634393406	38.0	35.0	41.0	27.0	41.0
37	35.67511630545785	38.0	35.0	41.0	27.0	41.0
38	35.579569437710816	38.0	35.0	41.0	27.0	41.0
39	35.50481004301497	38.0	35.0	40.0	27.0	41.0
40	35.43685877268086	38.0	35.0	40.0	26.0	41.0
41	35.33485450213012	38.0	34.0	40.0	26.0	41.0
42	35.30344841814262	38.0	34.0	40.0	26.0	41.0
43	35.26728283630587	38.0	34.0	40.0	26.0	41.0
44	35.144682958026884	38.0	34.0	40.0	25.0	41.0
45	35.06353011563496	38.0	34.0	40.0	25.0	41.0
46	35.0427591471277	38.0	34.0	40.0	25.0	41.0
47	34.990716194052176	38.0	34.0	40.0	25.0	41.0
48	34.84473762932858	38.0	34.0	40.0	24.0	41.0
49	34.770405289706325	37.0	34.0	40.0	24.0	41.0
50	34.7079005188616	37.0	34.0	40.0	24.0	41.0
51	34.291371218138494	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	3.0
15	4.0
16	23.0
17	77.0
18	173.0
19	389.0
20	658.0
21	1039.0
22	1582.0
23	2378.0
24	3549.0
25	5390.0
26	7462.0
27	8729.0
28	9295.0
29	10135.0
30	11720.0
31	14119.0
32	17436.0
33	22132.0
34	33014.0
35	46218.0
36	37107.0
37	47438.0
38	72984.0
39	131627.0
40	33.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.653033225709954	23.83792537883086	28.797953436555503	15.711087958903686
2	32.11454153471628	25.478270736412117	29.410065708715432	12.997122020156175
3	27.989437091899365	25.401111993645753	33.43242936570975	13.177021548745138
4	24.908657664813344	28.028841690477908	32.91831282299908	14.144187821709664
5	22.739754288602583	32.254417544330174	31.182241110755804	13.82358705631144
6	21.66716524143053	40.47883808010893	27.78808165623098	10.065915022229557
7	88.34944245587614	2.4816644832530455	7.725364389383452	1.4435286714873687
8	88.65890265413697	2.107011336558596	7.526690942099997	1.7073950672044398
9	83.322777302126	5.246381894515334	9.061200911876051	2.369639891482624
10	38.011615072774724	38.688095066172906	13.985744200200118	9.314545660852254
11	27.65377593018578	24.081573708261555	31.519140113262434	16.745510248290234
12	28.79444622097521	22.970817903304003	30.374756300093868	17.859979575626912
13	23.118739878072684	27.111395356033956	31.745871285188205	18.023993480705155
14	17.647689879619982	30.575080201768053	32.08173875370063	19.69549116491134
15	17.204130262112788	28.17862042643615	37.676985445055344	16.940263866395718
16	21.394427653363316	26.64741136544155	35.70118523255934	16.256975748635796
17	20.97645007891235	26.74808908327574	30.663379511671806	21.612081326140103
18	21.396903334949403	26.924687703083254	33.214569386134116	18.463839575833223
19	23.706095334371742	28.620323282753784	29.670012275254532	18.003569107619942
20	25.504265393066028	27.551241451162024	29.857751462199438	17.086741693572513
21	23.54496972447727	27.40909606676088	31.554212269065328	17.49172193969652
22	22.78493547754866	24.73288427220119	31.80116150727747	20.68101874297267
23	20.09634527505854	28.02842907688023	31.667887315226473	20.20733833283476
24	19.19375303013111	27.273139886324955	35.087009892411004	18.446097191132935
25	20.197435606490412	29.034587334825616	31.48798778663751	19.27998927204646
26	19.729944400317713	31.195032132283917	29.512600187739185	19.56242327965918
27	18.69325273614392	30.8174906904057	31.735968558843858	18.75328801460652
28	17.974892462581103	29.048409890347937	34.406610069834855	18.57008757723611
29	18.91214424971375	26.84773526711573	33.96346306592534	20.276657417245186
30	20.44954251467357	28.205234003486584	32.555212857039706	18.79001062480014
31	22.1889151356983	28.746583043644204	30.132139504657374	18.932362316000123
32	22.956582734184007	27.986136183117914	30.763025695511796	18.294255387186283
33	22.1975800212496	28.603818738846538	30.262731708323447	18.935869531580412
34	19.587180095520047	28.463323808836122	31.58474567529373	20.364750420350102
35	19.863837512765233	28.57844300258915	31.657571975284444	19.900147509361172
36	22.421629204790445	28.951858308490557	29.77605396985858	18.850458516860424
37	20.08231641273738	30.101606098428974	30.93941800852047	18.876659480313172
38	20.220335661161716	29.764088175525828	29.81793425002321	20.197641913289253
39	19.54096737257976	29.998246392209854	29.750471926802348	20.710314308408034
40	22.091950940243237	28.2714584859144	29.70322766986786	19.9333629039745
41	18.946803791918963	27.698750812333024	30.73517427766832	22.619271118079695
42	20.717741353166293	28.011305612576464	29.58418864693686	21.686764387320384
43	20.75652703134832	28.313751379676717	29.564589501047006	21.365132087927957
44	19.923666484428995	29.849499190245815	29.61018330359077	20.616651021734423
45	19.129179002094013	30.932403577359892	28.7608182127642	21.177599207781892
46	20.896196734163375	30.447582600084587	28.44001114056714	20.2162095251849
47	20.041673973365793	28.323241492423385	30.682566043963977	20.952518490246845
48	20.332360252932137	27.668423712903454	31.883684226813696	20.115531807350713
49	20.155142712728097	27.262618239584086	31.294059395727388	21.28817965196043
50	18.895227092208824	29.596773361666134	31.025654250435824	20.48234529568922
51	18.838699029326513	29.648762674973955	30.240244267249828	21.272294028449707
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	231.0
1	431.0
2	631.0
3	3911.0
4	7191.0
5	4978.5
6	2766.0
7	2762.5
8	2759.0
9	2851.0
10	2943.0
11	3078.0
12	3213.0
13	3177.0
14	3141.0
15	2943.5
16	2746.0
17	2756.5
18	2767.0
19	2543.5
20	2320.0
21	2594.5
22	2869.0
23	3297.5
24	3726.0
25	4014.0
26	5392.5
27	6483.0
28	7805.5
29	9128.0
30	10572.5
31	12017.0
32	13837.5
33	15658.0
34	17716.5
35	19775.0
36	20683.0
37	21591.0
38	23525.5
39	25460.0
40	29096.5
41	32733.0
42	35812.0
43	38891.0
44	40551.5
45	42212.0
46	42093.5
47	41975.0
48	40136.0
49	38297.0
50	35085.0
51	31873.0
52	28978.5
53	26084.0
54	23127.0
55	20170.0
56	18112.5
57	16055.0
58	14764.0
59	13473.0
60	11902.0
61	10331.0
62	8803.0
63	7275.0
64	6177.5
65	5080.0
66	4181.5
67	3283.0
68	2715.0
69	2147.0
70	1727.5
71	1308.0
72	1032.5
73	757.0
74	599.0
75	364.5
76	288.0
77	241.0
78	194.0
79	129.0
80	64.0
81	49.0
82	34.0
83	24.0
84	14.0
85	11.0
86	8.0
87	8.0
88	8.0
89	4.5
90	1.0
91	1.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	484715.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.275848407693836
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.35440484824387	23.086293701688458
2	8.123160971030112	5.568564680686463
3	4.420260774575955	4.545245646955224
4	3.208319620111113	4.398715077694336
5	2.6139092169742995	4.479697803624239
6	2.1540508473859665	4.429915218447951
7	1.7655849315751913	4.236184502590582
8	1.540279453612804	4.2235508046014365
9	1.2978121395837816	4.0035250942234635
>10	7.502159572421185	39.212703342462966
>50	0.009724908841571263	0.2314556724182047
>100	0.009724908841571248	0.6364510164154944
>500	0.0	0.0
>1k	6.07806802598203E-4	0.9476974381911896
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4549	0.9384896279256884	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.0630679884055578E-4	0.0	0.0	0.0391982917797056	0.0
2	2.0630679884055578E-4	0.0	0.0	0.11532550055187069	0.0
3	2.0630679884055578E-4	0.0	0.0	0.23766543226432027	0.0
4	2.0630679884055578E-4	0.0	0.0	0.3767162146828549	0.0
5	2.0630679884055578E-4	0.0	0.0	0.6583249951002135	0.0
6	2.0630679884055578E-4	0.0	0.0	1.2068947732172515	0.0
7	2.0630679884055578E-4	0.0	0.0	1.4930423032091023	0.0
8	2.0630679884055578E-4	0.0	0.0	2.101853666587582	0.0
9	2.0630679884055578E-4	0.0	0.0	2.414614773629865	0.0
10	2.0630679884055578E-4	0.0	0.0	2.7863796251405466	0.0
11	2.0630679884055578E-4	0.0	0.0	3.1222470936529714	0.0
12	2.0630679884055578E-4	0.0	0.0	3.397047749708592	0.0
13	2.0630679884055578E-4	0.0	0.0	3.538986827310894	0.0
14	2.0630679884055578E-4	0.0	0.0	3.6297618188007386	0.0
15	2.0630679884055578E-4	0.0	0.0	3.7122845383369607	0.0
16	2.0630679884055578E-4	0.0	0.0	3.8426704352041923	0.0
17	2.0630679884055578E-4	0.0	0.0	4.014317691839534	0.0
18	2.0630679884055578E-4	0.0	0.0	4.2032947195774835	0.0
19	2.0630679884055578E-4	0.0	0.0	4.348328399162394	0.0
20	2.0630679884055578E-4	0.0	0.0	4.495631453534552	0.0
21	2.0630679884055578E-4	0.0	0.0	4.669135471359459	0.0
22	2.0630679884055578E-4	0.0	0.0	4.879568406176825	0.0
23	2.0630679884055578E-4	0.0	0.0	5.081130148644049	0.0
24	2.0630679884055578E-4	0.0	0.0	5.247000814911855	0.0
25	2.0630679884055578E-4	0.0	0.0	5.389352506111839	0.0
26	2.0630679884055578E-4	0.0	0.0	5.53459249249559	0.0
27	2.0630679884055578E-4	0.0	0.0	5.684990148850355	0.0
28	2.0630679884055578E-4	0.0	0.0	5.834768884808599	0.0
29	2.0630679884055578E-4	0.0	0.0	6.017556708581331	0.0
30	2.0630679884055578E-4	0.0	0.0	6.231703165777828	0.0
31	2.0630679884055578E-4	0.0	0.0	6.427075704279835	0.0
32	2.0630679884055578E-4	0.0	0.0	6.618115800006189	0.0
33	2.0630679884055578E-4	0.0	0.0	6.804823452956892	0.0
34	2.0630679884055578E-4	0.0	0.0	6.997307696275131	0.0
35	2.0630679884055578E-4	0.0	0.0	7.201964040724962	0.0
36	2.0630679884055578E-4	0.0	0.0	7.396305045232766	0.0
37	2.0630679884055578E-4	0.0	0.0	7.599723548889553	0.0
38	2.0630679884055578E-4	0.0	0.0	7.831406083987498	0.0
39	2.0630679884055578E-4	0.0	0.0	8.15530775816717	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGAATG	35	1.2099918E-7	45.000004	1
GCGCGAC	30	2.1628948E-6	45.000004	9
GAACGCC	20	7.0292444E-4	45.000004	28
GCGATAT	30	2.1628948E-6	45.000004	9
GCGATAC	60	0.0	45.000004	9
CGATACG	20	7.0292444E-4	45.000004	10
TTAAGCG	25	3.8874554E-5	45.0	1
CGAGGGT	25	3.8874554E-5	45.0	4
TGTTACG	25	3.8874554E-5	45.0	1
CGTTTTT	2580	0.0	42.034885	1
TTAGCGG	70	0.0	41.785717	2
CGACGTT	65	0.0	41.538464	27
TCGACGT	65	0.0	41.538464	26
GGCGATA	170	0.0	41.02941	8
AACGGGA	205	0.0	40.609756	4
GGCCGAT	100	0.0	40.5	8
CATACGA	100	0.0	40.5	18
AATACGG	50	1.0786607E-9	40.5	2
ACGGGAT	290	0.0	40.344826	5
GGACTCG	45	1.9243089E-8	40.0	8
>>END_MODULE